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Using subsite coupling to predict signal peptides
Abstract
Given a nascent protein sequence, how can one predict its signal peptide or "Zipcode" sequence? This is a first important problem for scientists to use signal peptides as a vehicle to find new drugs or to reprogram cells for gene therapy. Based on a model that takes into account the coupling effect among some key subsites, the so-called [-3, -1, +1] coupling model, a new prediction algorithm is developed. The overall rate of correct prediction for 1939 secretory proteins and 1440 non-secretary proteins was over 92%. It has not escaped our attention that the new method may also serve as a useful tool for helping investigate further many unclear details regarding the molecular mechanism of the ZIP code protein-sorting system in cells.
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Metadata
- Authors
- Chou, K.-C.
- Corresponding author
- Chou, Kuo-Chen
- ORCID
- Chou, Kuo-Chen
- Venue
- Protein Engineering, Design and Selection
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-02
- Volume
- 14
- Issue
- 2
- Pages
- 75-79
- ISSN
- 0269-2139 (ISSN-L)
- 1741-0134
- 1741-0126
- OpenAlex
- W1979648586
- Cited by
- 255
- References
- 20
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 9 · 2021: 12 · 2020: 26 · 2019: 34 · 2018: 34 · 2017: 16 · 2016: 16 · 2015: 21 · 2014: 18 · 2013: 9 · 2012: 6
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Protein Engineering Design and Selection (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Machine Learning in Bioinformatics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Keywords
- signal peptide prediction, signal peptide, secreted proteins, protein sorting, prediction algorithm
- MeSH terms
- Algorithms, Amino Acid Sequence, Animals, Automation, Computer Simulation, Eukaryotic Cells, Humans, Membrane Proteins, Predictive Value of Tests, Prokaryotic Cells, Proteins, Serine Endopeptidases, Structure-Activity Relationship, Models, Statistical, Sequence Alignment, Computer-Aided Design, Protein Transport, Protein Sorting Signals
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