The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast cancer cell line
Open accessAbstract
Development of an estrogen receptor-mediated, chemical-activated luciferase reporter gene-expression (ER-CALUX) assay was attempted by stable transfection of luciferase reporter genes in a number of cell lines. Stable transfection of the chimeric Gal4 estrogen receptor and luciferase gene constructs in MCF-7 breast cancer and Hepa.1c1c7 mouse hepatoma cell lines, as well as transfection of a newly constructed luciferase reporter gene pEREtata-Luc in the ECC-1 human endometrial cell line, resulted in constitutive, non-estradiol-inducible clones. Stable transfection of pEREtata-Luc in the T47D breast cancer cell line, however, resulted in an extremely sensitive, highly responsive cell line. Following a 24-h exposure to estradiol (E2), stably transfected T47D.Luc cells demonstrated a detection limit of 0.5 pM, an EC50 of 6 pM, and a maximum induction of 100-fold relative to solvent controls. No clear reduction in responsiveness has been found over extended culture periods (50 passages). Anti-estrogens ICI 182,780, TCDD, and tamoxifen inhibited the estradiol-mediated luciferase induction. Genistein, nonylphenol, and o,p'DDT were the most potent (pseudo-)estrogens tested in this system (EC50 100, 260, and 660 nM, respectively). Determination of interactive effects of the (pseudo-)estrogens nonylphenol, o,p'DDT, chlordane, endosulfan, dieldrin, and methoxychlor revealed that, in combination with 3 pM E2, (pseudo-)estrogens were additive. Slightly more than additive effects (less than 2-fold) were found for combinations of dieldrin and endosulfan tested in the range of 3 to 6 microM. At these concentrations, the combination of endosulfan and chlordane demonstrated additive interaction. The ER-CALUX assay with T47D cells can provide a sensitive, responsive, and rapid in vitro system to detect and measure substances with potential (anti-)estrogenic activity.
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Berthois, Y., Katzenellenbogen, J. A., & Katzenellenbogen, B. S. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.8.2496 · Cited by 1,307
Aarts, J. M. M. J. G., Denison, M. S., Cox, M. A., Schalk, M. A. C., Garrison, P. M., Tullis, K., de Haan, L. H. J., & Brouwer, A. (1995) · European Journal of Pharmacology: Environmental Toxicology and Pharmacology · doi:10.1016/0926-6917(95)90067-5 · Cited by 181
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Meerts, I. A., Letcher, R. J., Hoving, S., Marsh, G., Bergman, A., Lemmen, J. G., van der Burg, B., & Brouwer, A. (2001) · Environmental Health Perspectives · doi:10.1289/ehp.01109399 · Cited by 732
Self-citationVethaak, A. D., Lahr, J., Schrap, S. M., Belfroid, A. C., Rijs, G. B. J., Gerritsen, A., de Boer, J., Bulder, A. S., Grinwis, G. C. M., Kuiper, R. V., et al. (2005) · Chemosphere · doi:10.1016/j.chemosphere.2004.12.053 · Cited by 543
Self-citationTollefsen, K. E., Scholz, S., Cronin, M. T., Edwards, S. W., de Knecht, J., Crofton, K., Garcia-Reyero, N., Hartung, T., Worth, A., & Patlewicz, G. (2014) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2014.09.009 · Cited by 377
Sonneveld, E. (2004) · Toxicological Sciences · doi:10.1093/toxsci/kfi005 · Cited by 351
Are brominated flame retardants endocrine disruptors?
Legler, J. (2003) · Environment International · doi:10.1016/s0160-4120(03)00104-1 · Cited by 349
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Metadata
- Authors
- Legler, J., van den Brink, C. E., Brouwer, A., Murk, A. J., van der Saag, P. T., Vethaak, A. D., & van der Burg, B.
- Venue
- Toxicological Sciences
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-03-01
- Volume
- 48
- Issue
- 1
- Pages
- 55-66
- ISSN
- 1096-0929 (ISSN-L)
- 1096-6080
- OpenAlex
- W2144345410
- Cited by
- 430
- References
- 2
- FWCI
- 9.12
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Legler, J., van den Brink, C. E., Brouwer, A., Murk, A. J., van der Saag, P. T., Vethaak, A. D., & van der Burg, B. (1999). Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast cancer cell line. Toxicological Sciences. https://doi.org/10.1093/toxsci/48.1.55
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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