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Pathogen-Derived Resistance to a Potyvirus: Immune and Resistant Phenotypes in Transgenic Tobacco Expressing Altered Forms of a Potyvirus Coat Protein Nucleotide Sequence

Lindbo, John A.·1992·Molecular Plant-Microbe Interactions

Abstract

Transgenic Nicotiana tabacum 'Burley 49' plants containing one of six different forms of the tobacco etch virus (TEV) coat protein (CP) nucleotide sequence have been generated. In whole plant studies, R1 and R2 progeny were inoculated mechanically with TEV, and the appearance and severity of symptoms were recorded. Symptom phenotype was altered, ranging from near wild type susceptibility to apparent immunity. Protoplasts derived from wild type and transgenic Burley 49 plant lines were transfected with TEV RNA. Protoplasts from transgenic plants expressing full-length or truncated forms of TEV CP supported virus replication. Protoplasts from certain transgenic plants, producing plus- or minus-sense CP transcripts but no CP, did not support virus replication at wild type levels. A model is proposed to account for these observations.

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Metadata

Authors
Lindbo, J. A.
Venue
Molecular Plant-Microbe Interactions
Published
1992
Volume
5
Issue
2
Pages
144
ISSN
0894-0282 (ISSN-L)
OpenAlex
W2061807072
Cited by
215
References
0
FWCI
16.99
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Pathogen-Derived Resistance to a Potyvirus: Immune and Resistant Phenotypes in Transgenic Tobacco Expressing Altered Forms of a Potyvirus Coat Protein Nucleotide Sequence. https://app.cochat.ai/papers/10.1094/mpmi-5-144
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Lindbo, J. A. (1992). Pathogen-Derived Resistance to a Potyvirus: Immune and Resistant Phenotypes in Transgenic Tobacco Expressing Altered Forms of a Potyvirus Coat Protein Nucleotide Sequence. Molecular Plant-Microbe Interactions. https://doi.org/10.1094/mpmi-5-144

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