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Cloning and Heterologous Expression of the Phenazine Biosynthetic Locus from Pseudomonas aureofaciens 30-84
Abstract
Pseudomonas aureofaciens strain 30-84 suppresses take-all disease of wheat caused by Gaeumannomyces graminis var. tritici. Three antibiotics, phenazine-1-carboxylic acid, 2-hydroxyphenazine-1-carboxylic acid, and 2-hydroxyphenazine, were responsible for disease suppression. Tn5-induced mutants deficient in production of one or more of the antibiotics (Phz-) were significantly less suppressive than the parental strain. Cosmids pLSP259 and pLSP282 from a genomic library of strain 30-84 restored phenazine production and fungal inhibition to 10 different Phz- mutants. Sequences required for production of the phenazines were localized to a segment of approximately 2.8 kilobases that was present in both cosmids. Expression of this locus in Escherichia coli required the introduction of a functional promoter, was orientation-specific, and resulted in the production of all three phenazine antibiotics. These results strongly suggest that the cloned sequences encode a major portion of the phenazine biosynthetic pathway.
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Cited by
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Antibiotic production by bacterial biocontrol agents
Raaijmakers, J. M., Vlami, M., & de Souza, J. T. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020501420831 · Cited by 865
Ramamoorthy, V, Viswanathan, R., Raguchander, T., Prakasam, V, & Samiyappan, R. (2001) · Crop Protection · doi:10.1016/s0261-2194(00)00056-9 · Cited by 718
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Metadata
- Authors
- Pierson III, L. S.
- Venue
- Molecular Plant-Microbe Interactions
- Publisher
- Scientific Societies
- Published
- 1992
- Volume
- 5
- Issue
- 4
- Pages
- 330
- ISSN
- 0894-0282 (ISSN-L)
- OpenAlex
- W2327836954
- Cited by
- 256
- References
- 0
- FWCI
- 6.76
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pierson III, L. S. (1992). Cloning and Heterologous Expression of the Phenazine Biosynthetic Locus from Pseudomonas aureofaciens 30-84. Molecular Plant-Microbe Interactions. https://doi.org/10.1094/mpmi-5-330
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