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Goliath, a Ring-H2 Mitochondrial Protein, Regulated by Luteinizing Hormone/Human Chorionic Gonadotropin in Rat Leydig Cells1

Guais, A., Solhonne, B., Melaine, N., Guellaën, G., Bulle, F.·2004·Biology of Reproduction
DOI 10.1095/biolreprod.103.018820
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Abstract

We have cloned the rat homologue of the ring-H2 protein Goliath involved in Drosophila development. The rat Goliath mRNA (1.85 kb) was translated as a major ubiquitous protein species of 28-kDa and three larger isoforms (50, 46, and 36 kDa) expressed mainly in liver, lung, stomach, heart, and thymus and barely detectable in other tissues (kidney, skeletal muscle, brain, testis, intestine, and spleen). By immunohistochemistry on rat testis sections, we localized the protein in interstitial tissue and seminiferous tubules. In tubules, Goliath was expressed mainly in postmeiotic germ cells and to a much lesser extent in Sertoli cells. In the interstitium, Goliath was exclusively present in Leydig cells. Using a series of immunolabeling, cellular fractionation, and electron microscopy experiments, we established that Goliath is present in mitochondria of the R2C Leydig cell line. Using short-term hypophysectomized animals, we showed that Goliath is regulated by LH/hCG in Leydig cells but not in germ cells. This regulation in Leydig cells concerned only the 50-kDa isoform. This report is the first description of a differential regulation of the Goliath protein between germ cells and Leydig cells.

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Research works printed but not linked: 51

  1. 2002, Male germ cell gene expression., Recent Prog Horm Res, 57, 103, 10.1210/rp.57.1.103 · DOI
  2. 1991, List of cloned mouse genes with unique expression patterns during spermatogenesis., Mamm Genome, 1, 283, 10.1007/BF00352340 · DOI
  3. 1987, Differential expression of the mouse homeobox-containing gene Hox-1.4 during male germ cell differentiation and embryonic development., Proc Natl Acad Sci U S A, 84, 5813, 10.1073/pnas.84.16.5813 · DOI
  4. 1990, The two candidate testis-determining Y genes (Zfy-1 and Zfy-2) are differentially expressed in fetal and adult mouse tissues., Genes Dev, 4, 63, 10.1101/gad.4.1.63 · DOI
  5. 1995, Characterization of a large population of mRNAs from human testis., Genomics, 26, 151, 10.1016/0888-7543(95)80096-5 · DOI
  6. 1987, The goosberry-zipper region of drosophila: five genes encode different spatially restricted transcripts in the embryo., EMBO J, 6, 2793, 10.1002/j.1460-2075.1987.tb02575.x · DOI
  7. 1993, The Drosophila melanogaster developmental gene g1 encodes a variant zinc-finger-motif protein., Gene, 125, 205, 10.1016/0378-1119(93)90330-6 · DOI
  8. 2000, Cloning of murine G1RP, a novel gene related to Drosophila melanogaster g1., Gene, 248, 33, 10.1016/S0378-1119(00)00109-8 · DOI
  9. 1989, Effects of FSH and testosterone on highly purified rat Sertoli cells: inhibin alpha-subunit mRNA expression and inhibin secretion are enhanced by FSH but not by testosterone., J Endocrinol, 122, 757, 10.1677/joe.0.1220757 · DOI
  10. 1987, Effect of luteinizing hormone deprivation in situ on steroidogenesis of rat Leydig cells purified by a multistep procedure., Biol Reprod, 36, 769, 10.1095/biolreprod36.3.769 · DOI
  11. 1977, Dissociation of the mouse testis and characterization of isolated spermatogenic cells., J Histochem Cytochem, 25, 480, 10.1177/25.7.893996 · DOI
  12. 1993, Germ cell-conditioned medium contains multiple factors that modulate the secretion of testins, clusterin, and transferrin by Sertoli cells., J Androl, 14, 87
  13. 2001, hH-Rev107, a class II tumor suppressor gene, is expressed by post-meiotic testicular germ cells and CIS cells but not by human testicular germ cell tumors., Oncogene, 20, 5155, 10.1038/sj.onc.1204658 · DOI
  14. 1996, Macrophage migration inhibitory factor production by Leydig cells: evidence for a role in the regulation of testicular function., Endocrinology, 137, 5090, 10.1210/endo.137.11.8895383 · DOI
  15. 1987, Testosterone inhibits cAMP-induced de novo synthesis of Leydig cell cytochrome P-450(17 alpha) by an androgen receptor-mediated mechanism., J Biol Chem, 262, 11200, 10.1016/S0021-9258(18)60944-5 · DOI
  16. 1993, Dual subcellular localization of the 3 beta-hydroxysteroid dehydrogenase isomerase: characterization of the mitochondrial enzyme in the bovine adrenal cortex., J Steroid Biochem Mol Biol, 46, 773, 10.1016/0960-0760(93)90318-Q · DOI
  17. 1986, Application of cryoultramicrotomy to immunocytochemistry., J Microsc, 143, 139
  18. 1981, Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections., J Cell Biol, 89, 653, 10.1083/jcb.89.3.653 · DOI
  19. 1988, Localization of macromolecular components by application of the immunogold technique on cryosectioned bacteria., Methods Microbiol, 20, 211, 10.1016/S0580-9517(08)70053-9 · DOI
  20. 1997, Immunogold labeling of ultrathin sections: application in immunology., 1
  21. 2000, The PA domain: a protease-associated domain., Protein Sci, 9, 1930, 10.1110/ps.9.10.1930 · DOI
  22. 2001, The protease-associated domain: a homology domain associated with multiple classes of proteases., Trends Biochem Sci, 26, 147, 10.1016/S0968-0004(00)01768-0 · DOI
  23. 2000, Construction of macromolecular assemblages in eukaryotic processes and their role in human disease: linking RINGs together., Curr Protein Pept Sci, 1, 49, 10.2174/1389203003381478 · DOI
  24. 1998, Local regulation of macrophage subsets in the adult rat testis: examination of the roles of the seminiferous tubules, testosterone, and macrophage-migration inhibitory factor., Biol Reprod, 59, 371, 10.1095/biolreprod59.2.371 · DOI
  25. 1993, The Sertoli Cells, 155
  26. 1992, Structural changes in rat Leydig cells posthypophysectomy: a morphometric and endocrine study., Endocrinology, 131, 498, 10.1210/endo.131.1.1612031 · DOI
  27. 1996, Pituitary control of proliferation and differentiation of Leydig cells and their putative precursors in immature hypophysectomized rat testis., J Androl, 17, 639, 10.1002/j.1939-4640.1996.tb01848.x · DOI
  28. 1985, Regulation of steroidogenesis in rat Leydig cells in culture: effect of human chorionic gonadotropin and dibutyryl cyclic AMP on the synthesis of cholesterol side chain cleavage cytochrome P-450 and adrenodoxin., Arch Biochem Biophys, 238, 378, 10.1016/0003-9861(85)90178-X · DOI
  29. 1992, Structural manifestations of the rat Sertoli cell to hypophysectomy: a correlative morphometric and endocrine study [published erratum appears in Endocrinology 1994; 134(1):300]., Endocrinology, 131, 485, 10.1210/en.131.1.485 · DOI
  30. 1996, Computational method to predict mitochondrially imported proteins and their targeting sequences., Eur J Biochem, 241, 779, 10.1111/j.1432-1033.1996.00779.x · DOI
  31. 2002, Extensive feature detection of N-terminal protein sorting signals., Bioinformatics, 18, 298, 10.1093/bioinformatics/18.2.298 · DOI
  32. 2000, Predicting subcellular localization of proteins based on their N-terminal amino acid sequence., J Mol Biol, 300, 1005, 10.1006/jmbi.2000.3903 · DOI
  33. 1999, An internal targeting signal directing proteins into the mitochondrial intermembrane space., Proc Natl Acad Sci U S A, 96, 11752, 10.1073/pnas.96.21.11752 · DOI
  34. 2001, The N-terminal domain of rat liver carnitine palmitoyltransferase 1 contains an internal mitochondrial import signal and residues essential for folding of its C-terminal catalytic domain., J Biol Chem, 276, 5403, 10.1074/jbc.M009555200 · DOI
  35. 1999, Two alternatively spliced Meig1 messenger RNA species are differentially expressed in the somatic and in the germ-cell compartments of the testis., Cell Growth Differ, 10, 19
  36. 1990, Transcription of the rat and mouse proenkephalin genes is initiated at distinct sites in spermatogenic and somatic cells., Mol Cell Biol, 10, 3717, 10.1128/MCB.10.7.3717 · DOI
  37. 1991, Testis-specific transcripts of rat farnesyl pyrophosphate synthetase are developmentally regulated and localized to haploid germ cells., Biol Reprod, 44, 663, 10.1095/biolreprod44.4.663 · DOI
  38. 1997, The enzymatic activity of Cu/Zn superoxide dismutase does not fluctuate in mouse spermatogenic cells despite mRNA changes., Exp Cell Res, 232, 371, 10.1006/excr.1997.3524 · DOI
  39. 2002, Reproductive system: aromatase and estrogens., Mol Cell Endocrinol, 193, 137, 10.1016/S0303-7207(02)00107-7 · DOI
  40. 2001, Estrogen and spermatogenesis., Endocr Rev, 22, 289
  41. 1997, Luteinizing hormone on Leydig cell structure and function., Histol Histopathol, 12, 869
  42. 1993, Hormonal control of apoptotic cell death in the testis: gonadotropins and androgens as testicular cell survival factors., Mol Endocrinol, 7, 643
  43. 1990, Reversal of long-term LH deprivation on testosterone secretion and Leydig cell volume, number and proliferation in adult rats., J Endocrinol, 127, 47, 10.1677/joe.0.1270047 · DOI
  44. 1999, Leydig cell apoptosis after the administration of ethane dimethanesulfonate to the adult male rat is a Fas-mediated process., Endocrinology, 140, 3797, 10.1210/endo.140.8.6919 · DOI
  45. 1993, Temporal and stage-specific changes in spermatogenesis of rat after gonadotropin deprivation by a potent gonadotropin-releasing hormone antagonist treatment., Endocrinology, 133, 2161, 10.1210/endo.133.5.8404667 · DOI
  46. 1991, Further observations of stage-specific effects seen after short-term hypophysectomy in the rat., Tissue Cell, 23, 613, 10.1016/0040-8166(91)90018-O · DOI
  47. 1992, Spontaneous degeneration of germ cells in normal rat testis: assessment of cell types and frequency during the spermatogenic cycle., J Reprod Fertil, 95, 825, 10.1530/jrf.0.0950825 · DOI
  48. 1978, The morphology and kinetics of spermatogonial degeneration in normal adult rats: an analysis using a simplified classification of the germinal epithelium., Anat Rec, 190, 905, 10.1002/ar.1091900410 · DOI
  49. 2001, Origin, differentiation and regulation of fetal and adult Leydig cells., Mol Cell Endocrinol, 179, 47, 10.1016/S0303-7207(01)00461-0 · DOI
  50. 1995, Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stages., Endocrinology, 136, 5, 10.1210/endo.136.1.7828558 · DOI
  51. 1996, Spontaneous germ cell death in the testis of the adult rat takes the form of apoptosis: re-evaluation of cell types that exhibit the ability to die during spermatogenesis., Cell Prolif, 29, 13, 10.1111/j.1365-2184.1996.tb00091.x · DOI

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Metadata

Authors
Guais, A., Solhonne, B., Melaine, N., Guellaën, G., & Bulle, F.
Venue
Biology of Reproduction
Publisher
Oxford University Press (OUP)
Published
2004-01-01
Volume
70
Issue
1
Pages
204-213
ISSN
  • 0006-3363
  • 1529-7268
DOI
10.1095/biolreprod.103.018820
Cited by
12
References
0
Language
en

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