The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Efficient production of chicken egg yolk antibodies against a conserved mammalian protein
Abstract
The egg yolk of immunized chicken is a rich and inexpensive source of specific polyclonal antibodies. In this paper we show that 20‐30 μg of a highly conserved mammalian protein, as exemplified by proliferating cell nuclear antigen, are sufficient to induce an immune response. Immunoblot analysis revealed that specific antibodies appeared 20 days after immunization, reached a plateau after 30 days, and remained high until at least day 81. A total amount of 4 g immunoglobulin was extracted from 62 eggs of one immunized hen, yielding approximately 130 mg of specific antibodies.— G assmann , M.; T hömmes , P.; W eiser , T.; H übscher , U. Efficient production of chicken egg yolk antibodies against a conserved mammalian protein. FASEB J. 4: 2528‐2532; 1990.
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Cites
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4350 · Cited by 53,385
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein
Prelich, G., Tan, C.-K., Kostura, M., Mathews, M. B., So, A. G., Downey, K. M., & Stillman, B. (1987) · Nature · doi:10.1038/326517a0 · Cited by 1,059
Prelich, G., Kostura, M., Marshak, D. R., Mathews, M. B., & Stillman, B. (1987) · Nature · doi:10.1038/326471a0 · Cited by 482
PHYLOGENY OF IMMUNOGLOBULIN STRUCTURE AND FUNCTION
Leslie, G. A., & Clem, L. W. (1969) · The Journal of Experimental Medicine · doi:10.1084/jem.130.6.1337 · Cited by 383
The Chicken Egg, an Antibody Source
LÖSCH, U., Schranner, I., Wanke, R., & Jürgens, L. (1986) · Journal of Veterinary Medicine Series B · doi:10.1111/j.1439-0450.1986.tb00076.x · Cited by 136
Patterson, R., Youngner, J. S., Weigle, W. O., & Dixon, F. J. (1962) · The Journal of General Physiology · doi:10.1085/jgp.45.3.501 · Cited by 114
Yolken, R. H., Leister, F. J., Wee, S.-B., Miskuff, R. L., & Vonderfecht, S. L. (1988) · PEDIATRICS · doi:10.1542/peds.81.2.291 · Cited by 94
Antibodies to Proteins from Yolk of Immunized Hens
Polson, A., von Wechmar, M. B., & Fazakerley, G. (1980) · Immunological Communications · doi:10.3109/08820138009066011 · Cited by 92
Burgers, P. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.14.6297 · Cited by 68
Cited by
Schade, R., Calzado, E. G., Sarmiento, R., Chacana, P. A., Porankiewicz-Asplund, J., & Terzolo, H. R. (2005) · Alternatives to Laboratory Animals · doi:10.1177/026119290503300208 · Cited by 381
Huang, T. G., & Hackney, D. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34033-4 · Cited by 174
McGuire, J., Whitelaw, M. L., Pongratz, I., Gustafsson, J. A., & Poellinger, L. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.4.2438 · Cited by 51
Metadata
- Authors
- Gassmann, M., Thömmes, P., Weiser, T., & Hübscher, U.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 1990-05
- Volume
- 4
- Issue
- 8
- Pages
- 2528-2532
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1568329054
- Cited by
- 379
- References
- 9
- FWCI
- 12.85
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gassmann, M., Thömmes, P., Weiser, T., & Hübscher, U. (1990). Efficient production of chicken egg yolk antibodies against a conserved mammalian protein. The FASEB Journal. https://doi.org/10.1096/fasebj.4.8.1970792
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
