The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.
In vivo activation of JAK2/STAT‐3 pathway during angiogenesis induced by GM‐CSF
Abstract
Besides the regulation of hematopoiesis, granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) induces the expression of a functional program in cultured endothelial cells (ECs) related to angiogenesis and to the their survival in bone marrow microenvironment. ECs express the specific GM‐CSF receptor that signals through the recruitment and the activation of Janus kinase (JAK)2 (Soldi et al., Blood 89 , 863–872, 1987). We now report that GM‐CSF in vivo induces angiogenesis and activates JAK‐2 and signal transducers and activators of transcription (STAT)‐3. This cytokine has an angiogenetic activity in chick chorioallantoic membrane (CAM) without recruitment of inflammatory cells and induces vessel sprouting from chicken aorta rings. When added to CAM, subnanomolar concentrations of GM‐CSF cause a rapid phosphorylation in tyrosine residues of JAK‐2 persisting at least for 10 min. Furthermore, we show that signal transducers and activators of transcription (STAT)‐3, but not STAT‐5, also are phosphorylated for 30 min after GM‐CSF stimulation. AG‐490, a JAK‐2 inhibitor, reduced in a dose‐dependent manner the angiogenic effect of GM‐CSF in CAM. These findings provide the first evidence that the JAK‐2/STAT‐3 pathway is activated in vivo and participates in vessel formation triggered by GM‐CSF.
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“STAT3 is a critical regulator of the tumor microenvironment.”
From Critical analysis of the potential for targeting STAT3 in human malignancy (2013) · cites this paper as [89]
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Metadata
- Authors
- Valdembri, D., Serini, G., Vacca, A., Ribatti, D., & Bussolino, F.
- Affiliations
- University of Turin (IT)
- University of Bari Aldo Moro (IT)
- Department of Human Anatomy and Histology University of Bari Bari Italy
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2001-12-14
- Volume
- 16
- Issue
- 2
- Pages
- 1-19
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1964106770
- Cited by
- 144
- References
- 69
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 6 · 2021: 5 · 2020: 5 · 2019: 5 · 2018: 8 · 2017: 2 · 2016: 2 · 2015: 9 · 2014: 8 · 2013: 7 · 2012: 6
- FWCI
- 3.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
- Funders
- Associazione Italiana per la Ricerca sul Cancro
- Istituto Superiore di Sanità
- Associazione Italiana per la Lotta al Neuroblastoma
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
- Keywords
- GM-CSF, JAK2/STAT3 signaling, angiogenesis, endothelial cells, JAK2 phosphorylation, STAT3 phosphorylation, STAT5, AG490, JAK2 inhibitors, chorioallantoic membrane, vessel sprouting, bone marrow microenvironment, endothelial cell survival
- MeSH terms
- Allantois, Animals, Aorta, Blood Vessels, Chick Embryo, Chickens, Chorion, DNA-Binding Proteins, Endothelium, Vascular, Phosphorylation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Tyrosine, Signal Transduction, Trans-Activators, Granulocyte-Macrophage Colony-Stimulating Factor, Neovascularization, Physiologic, STAT3 Transcription Factor, Janus Kinase 2
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