Alteration of soluble adhesion molecules during aging and their modulation by calorie restriction
Abstract
To investigate the status of soluble adhesion molecules (sAMs) during aging, the present study determined protein levels of several major sAMs in serum samples obtained from rats at different ages. These sAMs include E-selectin, P-selectin, vascular cell adhesion molecule 1 (VCAM-1), and intercellular adhesion molecule 1 (ICAM-1). Fischer 344 rats, ages 6, 12, 18, and 24 months, fed ad libitum (AL) and calorie restricted (CR) diets were used in this study. Analysis by Western blotting showed that the levels of all sAMs studied increased during aging in AL rats, but were effectively blunted in the CR rats. Total reactive oxygen species/reactive nitrogen species (ROS/RNS) levels were measured by fluorescent probe 2',7'-dichlorofluorescin diacetate. Increased ROS/RNS levels were found to coincide with increased levels of superoxide-generating xanthine oxidase in serum during aging, but were found suppressed by CR. Increases in sAMs levels were duplicated in another experiment in which young (13-month-old) and old (31-month-old) rats were injected with proinflammatory lipopolysaccharide. These findings suggest that the altered expressions of sAMs may be due to increased oxidative stress with advanced age and that these increases were prevented by CR through its antioxidative action.
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NF-κB is a key transcription factor that promotes production of pro-inflammatory cytokines, including TNFα and interleukins, as well as iNOS and COX-2, during inflammation.
“Nuclear factor κ B (NF-κB) is a major transcriptional factors responsible for pro-inflammatory cytokine production, such as tumor necrosis factor α (TNFα), interleukins (ILs), inducible nitric oxide synthase and cyclooxygenase-2, under inflammatory conditions [9, 10].”
From Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NF-κB pathway in macrophages and splenocytes (2013) · cites this paper as [9]
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References 39 · Cited by 106
- Authors
- Zou, Y., Jung, K. J., Kim, J. W., Yu, B. P., & Chung, H. Y.
- Affiliations
- Pusan National University (KR)
- The University of Texas at San Antonio Health Science Center (US)
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2003-12-19
- Volume
- 18
- Issue
- 2
- Pages
- 320-322
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2119360937
- Cited by
- 106
- References
- 39
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 8 · 2017: 4 · 2016: 1 · 2015: 6 · 2014: 8 · 2013: 5 · 2012: 7
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Korean Foundation for Cancer Research (FS0004)
- Korea Science and Engineering Foundation (R21‐2000‐000‐00021‐0)
- National Institute on Aging (AG01188)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- soluble adhesion molecules, aging, caloric restriction, E-selectin, P-selectin, VCAM-1, ICAM-1, reactive oxygen species, reactive nitrogen species, oxidative stress, xanthine oxidase, superoxide, lipopolysaccharide, inflammation, antioxidant activity, serum protein levels
- MeSH terms
- Aging, Animals, Lipopolysaccharides, Rats, Inbred F344, Solubility, Xanthine Oxidase, Blotting, Western, Cell Adhesion Molecules, Reactive Oxygen Species, Oxidative Stress, Intercellular Adhesion Molecule-1, P-Selectin, Vascular Cell Adhesion Molecule-1, E-Selectin, Reactive Nitrogen Species, Caloric Restriction, Rats
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