The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Identification and characterization of a novel peptide ligand of epidermal growth factor receptor for targeted delivery of therapeutics
Abstract
Epidermal growth factor receptor (ErbB1, EGFR) is overexpressed in a variety of human cancer cells. It has been considered as a rational target for drug delivery. To identify novel ligands with specific binding capabilities to EGFR, we screened a phage display peptide library and found an enriched phage clone encoding the amino acid sequence YHWYGYTPQNVI (designated as GE11). Competitive binding assay and Scatchard analysis revealed that GE11 peptide bound specifically and efficiently to EGFR with a dissociation constant of ∼22 nM, but with much lower mitogenic activity than with EGF. We showed that the peptides were internalized preferentially into EGFR highly expressing cells, and they accumulated in EGFR overexpressing tumor xenografts after i.v. delivery in vivo. In gene delivery studies, GE11‐conjugated polyethylenimine (PEI) vectors were less mitogenic, but still quite efficient at transfecting genes into EGFR highly expressing cells and tumor xenografts. Taken together, GE11 is a potentially safe and efficient targeting moiety for selective drug delivery systems mediated through EGFR.—Li, Z., Zhao, R., Wu, X., Sun, Y., Yao, M., Li, J., Xu, Y., Gu, J. Identification and characterization of a novel peptide ligand of epidermal growth factor receptor for targeted delivery of therapeutics. FASEB J. 19, 1978–1985 (2005)
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Epidermal growth factor-related peptides and their receptors in human malignancies
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Crystal Structure of the Complex of Human Epidermal Growth Factor and Receptor Extracellular Domains
Ogiso, H., Ishitani, R., Nureki, O., Fukai, S., Yamanaka, M., Kim, J.-H., Saito, K., Sakamoto, A., Inoue, M., Shirouzu, M., et al. (2002) · Cell · doi:10.1016/s0092-8674(02)00963-7 · Cited by 1,227
The Epidermal Growth Factor Receptor and Its Inhibition in Cancer Therapy
Woodburn, J. R. (1999) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(98)00045-x · Cited by 827
Efficient Gene Transfer Using Reversibly Cross-Linked Low Molecular Weight Polyethylenimine
Gosselin, M. A., Guo, W., & Lee, R. J. (2001) · Bioconjugate Chemistry · doi:10.1021/bc0100455 · Cited by 454
Lee, E. R., Marshall, J., Siegel, C. S., Jiang, C., Yew, N. S., Nichols, M. R., Nietupski, J. B., Ziegler, R. J., Lane, M. B., Wang, K. X., et al. (1996) · Human Gene Therapy · doi:10.1089/hum.1996.7.14-1701 · Cited by 443
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Identification of a peptide blocking vascular endothelial growth factor (VEGF)-mediated angiogenesis
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Metadata
- Authors
- Li, Z., Zhao, R., Wu, X., Sun, Y., Yao, M., Li, J., Xu, Y., & Gu, J.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2005-12
- Volume
- 19
- Issue
- 14
- Pages
- 1978-1985
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2081477031
- Cited by
- 419
- References
- 25
- FWCI
- 1.98
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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