Tau associates with actin in differentiating PC12 cells
Abstract
The microtubule‐associated protein tau may be involved in cell morphogenesis and axonal maintenance. In addition to microtubules, tau has been shown to interact with actin in vitro . In the present study interaction of tau and actin was investigated in PC12 cells. No interaction between tau and actin was observed without NGF treatment. Under NGF stimulation, tau distributed at ends of cellular extensions, where it associated with actin in a microtubule‐independent manner. F‐actin disruption revealed that relocalization and assembly of F‐actin at the ends of cellular extensions were necessary for NGF‐induced tau reorganization and association with actin. A truncated tau‐GFP (tau(1–186)‐GFP, N‐terminal of tau) did not associate with actin. However, tau23(174–352)‐GFP (carboxyl‐terminal of Tau23) did associate with actin and the requirement for NGF was lost. Nevertheless, NGF boosted tau23(174–352)‐GFP interaction with actin and promoted colocalization at the ends of cellular extensions. This suggests that the C‐terminal of tau is required for associating with actin and the tau N‐terminal may play a regulatory role in this process. A possible role for tau‐actin interaction in neurite outgrowth is postulated—Yu, J.‐Z., Rasenick, M. M. Tau associates with actin in differentiating PC12 cells. FASEB J. 20, 1452–1461 (2006)
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Tau colocalizes with actin in differentiating PC12 cells, N2a cells and in the postsynaptic compartments of mature neurons.
“Tau colocalizes with actin in differentiating PC12 cells68, N2a cells69 and in the postsynaptic compartments of mature neurons70.”
From Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau (2017) · cites this paper as [68]
In vivo, tau co-localizes with actin in differentiating PC12 cells and in the post-synaptic compartments of mature neurons.
“In vivo, tau co-localizes with actin in differentiating PC12 cells22 and in the post-synaptic compartments of mature neurons23.”
From Tau co-organizes dynamic microtubule and actin networks (2015) · cites this paper as [22]
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Maas, T., Eidenmüller, J., & Brandt, R. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m000389200 · Cited by 226 · see citing works
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Morris, M., Maeda, S., Vossel, K., & Mucke, L. (2011) · Neuron · doi:10.1016/j.neuron.2011.04.009 · Cited by 899 · see citing works
Arendt, T., Stieler, J. T., & Holzer, M. (2016) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2016.08.018 · Cited by 609 · see citing works
Frandemiche, M. L., De Seranno, S., Rush, T., Borel, E., Elie, A., Arnal, I., Lanté, F., & Buisson, A. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.4261-13.2014 · Cited by 272 · see citing works
Tau co-organizes dynamic microtubule and actin networks
Elie, A., Prezel, E., Guérin, C., Denarier, E., Ramirez-Rios, S., Serre, L., Andrieux, A., Fourest-Lieuvin, A., Blanchoin, L., & Arnal, I. (2015) · Scientific Reports · doi:10.1038/srep09964 · Cited by 200 · see citing works
Tau reduction prevents Aβ-induced axonal transport deficits by blocking activation of GSK3β
Vossel, K. A., Xu, J. C., Fomenko, V., Miyamoto, T., Suberbielle, E., Knox, J. A., Ho, K., Kim, D. H., Yu, G.-Q., & Mucke, L. (2015) · Journal of Cell Biology · doi:10.1083/jcb.201407065 · Cited by 167 · see citing works
Cabrales Fontela, Y., Kadavath, H., Biernat, J., Riedel, D., Mandelkow, E., & Zweckstetter, M. (2017) · Nature Communications · doi:10.1038/s41467-017-02230-8 · Cited by 148 · see citing works
Tzezana, R., Reznik, S., Blumenthal, J., Zussman, E., & Levenberg, S. (2012) · Macromolecular Bioscience · doi:10.1002/mabi.201100312 · Cited by 11 · see citing works
- Authors
- Yu, J.-Z., & Rasenick, M. M.
- Affiliations
- University of Illinois Chicago (US)
- ORCID
- Rasenick, Mark M.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2006-07
- Volume
- 20
- Issue
- 9
- Pages
- 1452-1461
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2085156649
- Cited by
- 72
- References
- 1
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 6 · 2021: 7 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 3 · 2015: 7 · 2014: 6 · 2013: 1 · 2012: 7
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale (AG 15482, MH57391, MH 39595)
- University of Illinois at Chicago
- Stanford University
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- tau protein, actin, PC12 cells, nerve growth factor, neurite outgrowth, cell differentiation, F-actin, cell morphogenesis
- MeSH terms
- Actins, Animals, Cell Differentiation, Pheochromocytoma, Recombinant Fusion Proteins, Transfection, PC12 Cells, tau Proteins, Genes, Reporter, Green Fluorescent Proteins, Rats
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