The DOI matches its Crossref record; 77 of the 77 references it cites were found in a registry or an index.
SIRT1 is a redox‐sensitive deacetylase that is post‐translationally modified by oxidants and carbonyl stress
Open accessAbstract
Sirtuinl (SIRT1) deacetylase levels are decreased in chronic inflammatory conditions and aging where oxidative stress occurs. We determined the mechanism of SIRT1 redox post‐translational modifications leading to its degradation. Human lung epithelial cells exposed to hydrogen peroxide (150–250 µM), aldehyde‐acrolein (10–30 µM), and cigarette smoke extract (CSE;0.1–1.5%) in the presence of intracellular glutathione‐modulating agents at 1–24 h, and oxidative post‐translational modifications were assayed in cells, as well as in lungs of mice lacking and overexpressing glutaredoxin‐1 (Glrx1), and wild‐type (WT) mice in response to cigarette smoke (CS). CSE and aldehydes dose and time dependently decreased SIRT1 protein levels, with EC 50 of 1% for CSE and 30 µM for acrolein at 6 h, and >80% inhibition at 24 h with CSE, which was regulated by modulation of intracellular thiol status of the cells. CS decreased the lung levels of SIRT1 in WT mice, which was enhanced by deficiency of Glrx1 and prevented by overexpression of Glrx1. Oxidants, aldehydes, and CS induced carbonyl modifications on SIRT1 on cysteine residues concomitant with decreased SIRT1 activity. Proteomics studies revealed alkylation of cysteine residue on SIRT1. Our data suggest that oxidants/aldehydes covalently modify SIRT1, decreasing enzymatic activity and marking the protein for proteasomal degradation, which has implications in inflammatory conditions.—Caito, S., Rajendrasozhan, S., Cook, S., Chung, S., Yao, H., Friedman, A. E., Brookes, P. S., Rahman, I. SIRT1 is a redox‐sensitive deacetylase that is post‐translationally modified by oxidants and carbonyl stress. FASEB J . 24, 3145–3159 (2010). www.fasebj.org
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Cites
In-gel digestion for mass spectrometric characterization of proteins and proteomes
Shevchenko, A., Tomas, H., Havli, J., Olsen, J. V., & Mann, M. (2006) · Nature Protocols · doi:10.1038/nprot.2006.468 · Cited by 4,942
Global, In Vivo, and Site-Specific Phosphorylation Dynamics in Signaling Networks
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Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase
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Modulation of NF‐κB‐dependent transcription and cell survival by the SIRT1 deacetylase
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Negative Control of p53 by Sir2α Promotes Cell Survival under Stress
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Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase
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Cited by
Kauppinen, A., Suuronen, T., Ojala, J., Kaarniranta, K., & Salminen, A. (2013) · Cellular Signalling · doi:10.1016/j.cellsig.2013.06.007 · Cited by 989
Redox regulation of SIRT1 in inflammation and cellular senescence
Hwang, J.-W., Yao, H., Caito, S., Sundar, I. K., & Rahman, I. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2013.03.015 · Cited by 503
Self-citationSIRT1 protects against emphysema via FOXO3-mediated reduction of premature senescence in mice
Yao, H., Chung, S., Hwang, J.-W., Rajendrasozhan, S., Sundar, I. K., Dean, D. A., McBurney, M. W., Guarente, L., Gu, W., Rönty, M., et al. (2012) · Journal of Clinical Investigation · doi:10.1172/jci60132 · Cited by 358
Self-citationRegulation of SIRT1 in aging: Roles in mitochondrial function and biogenesis
Yuan, Y., Cruzat, V. F., Newsholme, P., Cheng, J., Chen, Y., & Lu, Y. (2016) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2016.02.003 · Cited by 299
Crosstalk between poly(ADP-ribose) polymerase and sirtuin enzymes
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Metadata
- Authors
- Caito, S., Rajendrasozhan, S., Cook, S., Chung, S., Yao, H., Friedman, A. E., Brookes, P. S., & Rahman, I.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2010-04-12
- Volume
- 24
- Issue
- 9
- Pages
- 3145-3159
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2113183122
- Cited by
- 294
- References
- 77
- FWCI
- 7.40
- Type
- journal article
- Language
- en
- Open access
- green
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- Caito, S., Rajendrasozhan, S., Cook, S., Chung, S., Yao, H., Friedman, A. E., Brookes, P. S., & Rahman, I. (2010). SIRT1 is a redox‐sensitive deacetylase that is post‐translationally modified by oxidants and carbonyl stress. The FASEB Journal. https://doi.org/10.1096/fj.09-151308
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