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Cited by 25Air Quality and Health ImpactsEpigenetics and DNA MethylationCardiac Fibrosis and Remodeling

In utero exposure to diesel exhaust particulates is associated with an altered cardiac transcriptional response to transverse aortic constriction and altered DNA methylation

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Goodson, Jamie M., Weldy, Chad S., MacDonald, James W., Liu, Yonggang, Bammler, Theo K., Chien, Wei‐Ming, Chin, Michael T.·2017·The FASEB Journal
DOI 10.1096/fj.201700032rPMC PMC5636696
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Abstract

In utero exposure to diesel exhaust air pollution has been associated with increased adult susceptibility to heart failure in mice, but the mechanisms by which this exposure promotes susceptibility to heart failure are poorly understood. To identify the potential transcriptional effects that mediate this susceptibility, we have performed RNA sequencing analysis on adult hearts from mice that were exposed to diesel exhaust in utero and that have subsequently undergone transverse aortic constriction. We identified 3 target genes, Mir133a‐2, Ptprf , and Pamr1 , which demonstrate dysregulation after exposure and aortic constriction. Examination of expression patterns in human heart tissues indicates a correlation between expression and heart failure. We subsequently assessed DNA methylation modifications at these candidate loci in neonatal cultured cardiac myocytes after in utero exposure to diesel exhaust and found that the promoter for Mir133a‐2 is differentially methylated. These target genes in the heart are the first genes to be identified that likely play an important role in mediating adult sensitivity to heart failure. We have also shown a change in DNA methylation within cardiomyocytes as a result of in utero exposure to diesel exhaust.—Goodson, J. M., Weldy, C. S., MacDonald, J. W., Liu, Y., Bammler, T. K., Chien, W.‐M., Chin, M. T. In utero exposure to diesel exhaust particulates is associated with an altered cardiac transcriptional response to transverse aortic constriction and altered DNA methylation. FASEB J. 31, 4935–4945 (2017). www.fasebj.org

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  1. [1]Pope C. A. III, Burnett R. T., Thun M. J., Calle E. E., Krewski D., Ito K., Thurston G. D. (2002) Lung cancer, cardiopulmonary mortality, and long-term exposure to fine particulate air pollution. JAMA. 287, 1132–1141 [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. [2]Ni L., Chuang C. C., Zuo L. (2015) Fine particulate matter in acute exacerbation of COPD. Front. Physiol. 6, 294 [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. [3]Brook R. D., Rajagopalan S., Pope C. A. III, Brook J. R., Bhatnagar A., Diez-Roux A. V., Holguin F., Hong Y., Luepker R. V., Mittleman M. A., Peters A., Siscovick D., Smith S. C. Jr., Whitsel L., Kaufman J. D.; American Heart Association Council on Epidemiology and Prevention, Council on the Kidney in Cardiovascular Disease, and Council on Nutrition, Physical Activity and Metabolism (2010) Particulate matter air pollution and cardiovascular disease: an update to the scientific statement from the American Heart Association. Circulation 121, 2331–2378 [DOI] [PubMed] [Google Scholar]
  4. [4]Krishnan R. M., Adar S. D., Szpiro A. A., Jorgensen N. W., Van Hee V. C., Barr R. G., O’Neill M. S., Herrington D. M., Polak J. F., Kaufman J. D. (2012) Vascular responses to long- and short-term exposure to fine particulate matter: MESA air (multi-ethnic study of atherosclerosis and air pollution). J. Am. Coll. Cardiol. 60, 2158–2166 [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. [5]Pope C. A. III, Muhlestein J. B., May H. T., Renlund D. G., Anderson J. L., Horne B. D. (2006) Ischemic heart disease events triggered by short-term exposure to fine particulate air pollution. Circulation 114, 2443–2448 [DOI] [PubMed] [Google Scholar]
  6. [6]Wang Y., Eliot M. N., Wellenius G. A. (2014) Short-term changes in ambient particulate matter and risk of stroke: a systematic review and meta-analysis. J. Am. Heart Assoc. 3, e000983 [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. [7]Van den Hooven E. H., Pierik F. H., de Kluizenaar Y., Willemsen S. P., Hofman A., van Ratingen S. W., Zandveld P. Y., Mackenbach J. P., Steegers E. A., Miedema H. M., Jaddoe V. W. (2012) Air pollution exposure during pregnancy, ultrasound measures of fetal growth, and adverse birth outcomes: a prospective cohort study. Environ. Health Perspect. 120, 150–156 [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. [8]Saenen N. D., Vrijens K., Janssen B. G., Madhloum N., Peusens M., Gyselaers W., Vanpoucke C., Lefebvre W., Roels H. A., Nawrot T. S. (2016) Placental nitrosative stress and exposure to ambient air pollution during gestation: a population study. Am. J. Epidemiol. 184, 442–449 [DOI] [PubMed] [Google Scholar]
  9. [9]Barker D. J., Osmond C., Golding J., Kuh D., Wadsworth M. E. (1989) Growth in utero, blood pressure in childhood and adult life, and mortality from cardiovascular disease. BMJ 298, 564–567 [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. [10]Van Rossem L., Rifas-Shiman S. L., Melly S. J., Kloog I., Luttmann-Gibson H., Zanobetti A., Coull B. A., Schwartz J. D., Mittleman M. A., Oken E., Gillman M. W., Koutrakis P., Gold D. R. (2015) Prenatal air pollution exposure and newborn blood pressure. Environ. Health Perspect. 123, 353–359 [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. [12]Weldy C. S., Liu Y., Liggitt H. D., Chin M. T. (2014) In utero exposure to diesel exhaust air pollution promotes adverse intrauterine conditions, resulting in weight gain, altered blood pressure, and increased susceptibility to heart failure in adult mice. PLoS One 9, e88582 [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. [13]Tarantini L., Bonzini M., Apostoli P., Pegoraro V., Bollati V., Marinelli B., Cantone L., Rizzo G., Hou L., Schwartz J., Bertazzi P. A., Baccarelli A. (2009) Effects of particulate matter on genomic DNA methylation content and iNOS promoter methylation. Environ. Health Perspect. 117, 217–222 [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. [14]Baccarelli A., Wright R. O., Bollati V., Tarantini L., Litonjua A. A., Suh H. H., Zanobetti A., Sparrow D., Vokonas P. S., Schwartz J. (2009) Rapid DNA methylation changes after exposure to traffic particles. Am. J. Respir. Crit. Care Med. 179, 572–578 [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. [16]Weldy C. S., Luttrell I. P., White C. C., Morgan-Stevenson V., Cox D. P., Carosino C. M., Larson T. V., Stewart J. A., Kaufman J. D., Kim F., Chitaley K., Kavanagh T. J. (2013) Glutathione (GSH) and the GSH synthesis gene Gclm modulate plasma redox and vascular responses to acute diesel exhaust inhalation in mice. Inhal. Toxicol. 25, 444–454 [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. [17]Gould T., Larson T., Stewart J., Kaufman J. D., Slater D., McEwen N. (2008) A controlled inhalation diesel exhaust exposure facility with dynamic feedback control of PM concentration. Inhal. Toxicol. 20, 49–52 [DOI] [PubMed] [Google Scholar]
  16. [18]Yin F., Lawal A., Ricks J., Fox J. R., Larson T., Navab M., Fogelman A. M., Rosenfeld M. E., Araujo J. A. (2013) Diesel exhaust induces systemic lipid peroxidation and development of dysfunctional pro-oxidant and pro-inflammatory high-density lipoprotein. Arterioscler. Thromb. Vasc. Biol. 33, 1153–1161 [DOI] [PubMed] [Google Scholar]
  17. [19]Liu Y., Chien W. M., Medvedev I. O., Weldy C. S., Luchtel D. L., Rosenfeld M. E., Chin M. T. (2013) Inhalation of diesel exhaust does not exacerbate cardiac hypertrophy or heart failure in two mouse models of cardiac hypertrophy. Part. Fibre Toxicol. 10, 49 [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. [20]Liao Y., Smyth G. K., Shi W. (2013) The subread aligner: fast, accurate and scalable read mapping by seed-and-vote. Nucleic Acids Res. 41, e108 [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. [21]Greco S., Fasanaro P., Castelvecchio S., D’Alessandra Y., Arcelli D., Di Donato M., Malavazos A., Capogrossi M. C., Menicanti L., Martelli F. (2012) MicroRNA dysregulation in diabetic ischemic heart failure patients. Diabetes 61, 1633–1641 [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. [22]Liu Y., Yu M., Wu L., Chin M. T. (2010) The bHLH transcription factor CHF1/Hey2 regulates susceptibility to apoptosis and heart failure after pressure overload. Am. J. Physiol. Heart Circ. Physiol. 298, H2082–H2092 [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. [23]Xiang F., Sakata Y., Cui L., Youngblood J. M., Nakagami H., Liao J. K., Liao R., Chin M. T. (2006) Transcription factor CHF1/Hey2 suppresses cardiac hypertrophy through an inhibitory interaction with GATA4. Am. J. Physiol. Heart Circ. Physiol. 290, H1997–H2006 [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. [24]Bolger A. M., Lohse M., Usadel B. (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114–2120 [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. [25]Krueger F., Andrews S. R. (2011) Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications. Bioinformatics 27, 1571–1572 [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. [26]Langmead B., Trapnell C., Pop M., Salzberg S. L. (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 10, R25 [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. [27]Hansen K. D., Langmead B., Irizarry R. A. (2012) BSmooth: from whole genome bisulfite sequencing reads to differentially methylated regions. Genome Biol. 13, R83 [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. [28]Feng H., Conneely K. N., Wu H. (2014) A bayesian hierarchical model to detect differentially methylated loci from single nucleotide resolution sequencing data. Nucleic Acids Res. 42, e69 [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. [29]Carè A., Catalucci D., Felicetti F., Bonci D., Addario A., Gallo P., Bang M. L., Segnalini P., Gu Y., Dalton N. D., Elia L., Latronico M. V., Høydal M., Autore C., Russo M. A., Dorn G. W. II, Ellingsen O., Ruiz-Lozano P., Peterson K. L., Croce C. M., Peschle C., Condorelli G. (2007) MicroRNA-133 controls cardiac hypertrophy. Nat. Med. 13, 613–618 [DOI] [PubMed] [Google Scholar]
  28. [30]Matkovich S. J., Wang W., Tu Y., Eschenbacher W. H., Dorn L. E., Condorelli G., Diwan A., Nerbonne J. M., Dorn G. W. II (2010) MicroRNA-133a protects against myocardial fibrosis and modulates electrical repolarization without affecting hypertrophy in pressure-overloaded adult hearts. Circ. Res. 106, 166–175 [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. [31]Brauer M., Freedman G., Frostad J., van Donkelaar A., Martin R. V., Dentener F., van Dingenen R., Estep K., Amini H., Apte J. S., Balakrishnan K., Barregard L., Broday D., Feigin V., Ghosh S., Hopke P. K., Knibbs L. D., Kokubo Y., Liu Y., Ma S., Morawska L., Sangrador J. L., Shaddick G., Anderson H. R., Vos T., Forouzanfar M. H., Burnett R. T., Cohen A. (2016) Ambient air pollution exposure estimation for the Global Burden of Disease 2013. Environ. Sci. Technol. 50, 79–88 [DOI] [PubMed] [Google Scholar]
  30. [32]Lewné M., Plato N., Gustavsson P. (2007) Exposure to particles, elemental carbon and nitrogen dioxide in workers exposed to motor exhaust. Ann. Occup. Hyg. 51, 693–701 [DOI] [PubMed] [Google Scholar]
  31. [33]Nakayama Y., Nara N., Kawakita Y., Takeshima Y., Arakawa M., Katoh M., Morita S., Iwatsuki K., Tanaka K., Okamoto S., Kitamura T., Seki N., Matsuda R., Matsuo M., Saito K., Hara T. (2004) Cloning of cDNA encoding a regeneration-associated muscle protease whose expression is attenuated in cell lines derived from Duchenne muscular dystrophy patients. Am. J. Pathol. 164, 1773–1782 [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. [34]Sharma P. R., Mackey A. J., Dejene E. A., Ramadan J. W., Langefeld C. D., Palmer N. D., Taylor K. D., Wagenknecht L. E., Watanabe R. M., Rich S. S., Nunemaker C. S. (2015) An islet-targeted genome-wide association scan identifies novel genes implicated in cytokine-mediated islet stress in type 2 diabetes. Endocrinology 156, 3147–3156 [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. [35]Lo P. H., Tanikawa C., Katagiri T., Nakamura Y., Matsuda K. (2015) Identification of novel epigenetically inactivated gene PAMR1 in breast carcinoma. Oncol. Rep. 33, 267–273 [DOI] [PubMed] [Google Scholar]
  34. [36]Tonks N. K. (2013) Protein tyrosine phosphatases--from housekeeping enzymes to master regulators of signal transduction. FEBS J. 280, 346–378 [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. [37]Chagnon M. J., Uetani N., Tremblay M. L. (2004) Functional significance of the LAR receptor protein tyrosine phosphatase family in development and diseases. Biochem. Cell Biol. 82, 664–675 [DOI] [PubMed] [Google Scholar]
  36. [38]Zhang W. R., Li P. M., Oswald M. A., Goldstein B. J. (1996) Modulation of insulin signal transduction by eutopic overexpression of the receptor-type protein-tyrosine phosphatase LAR. Mol. Endocrinol. 10, 575–584 [DOI] [PubMed] [Google Scholar]
  37. [39]Zabolotny J. M., Kim Y. B., Peroni O. D., Kim J. K., Pani M. A., Boss O., Klaman L. D., Kamatkar S., Shulman G. I., Kahn B. B., Neel B. G. (2001) Overexpression of the LAR (leukocyte antigen-related) protein-tyrosine phosphatase in muscle causes insulin resistance. Proc. Natl. Acad. Sci. USA 98, 5187–5192 [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. [40]Kulas D. T., Zhang W. R., Goldstein B. J., Furlanetto R. W., Mooney R. A. (1995) Insulin receptor signaling is augmented by antisense inhibition of the protein tyrosine phosphatase LAR. J. Biol. Chem. 270, 2435–2438 [DOI] [PubMed] [Google Scholar]
  39. [41]Menzaghi C., Paroni G., De Bonis C., Coco A., Vigna C., Miscio G., Lanna P., Tassi V., Bacci S., Trischitta V. (2008) The protein tyrosine phosphatase receptor type f (PTPRF) locus is associated with coronary artery disease in type 2 diabetes. J. Intern. Med. 263, 653–654 [DOI] [PubMed] [Google Scholar]
  40. [42]Ahmad F., Goldstein B. J. (1997) Functional association between the insulin receptor and the transmembrane protein-tyrosine phosphatase LAR in intact cells. J. Biol. Chem. 272, 448–457 [PubMed] [Google Scholar]
  41. [43]Liu N., Bezprozvannaya S., Williams A. H., Qi X., Richardson J. A., Bassel-Duby R., Olson E. N. (2008) microRNA-133a regulates cardiomyocyte proliferation and suppresses smooth muscle gene expression in the heart. Genes Dev. 22, 3242–3254 [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. [44]Drawnel F. M., Wachten D., Molkentin J. D., Maillet M., Aronsen J. M., Swift F., Sjaastad I., Liu N., Catalucci D., Mikoshiba K., Hisatsune C., Okkenhaug H., Andrews S. R., Bootman M. D., Roderick H. L. (2012) Mutual antagonism between IP(3)RII and miRNA-133a regulates calcium signals and cardiac hypertrophy. J. Cell Biol. 199, 783–798 [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. [45]He B., Xiao J., Ren A. J., Zhang Y. F., Zhang H., Chen M., Xie B., Gao X. G., Wang Y. W. (2011) Role of miR-1 and miR-133a in myocardial ischemic postconditioning. J. Biomed. Sci. 18, 22 [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. [46]Liu N., Bezprozvannaya S., Shelton J. M., Frisard M. I., Hulver M. W., McMillan R. P., Wu Y., Voelker K. A., Grange R. W., Richardson J. A., Bassel-Duby R., Olson E. N. (2011) Mice lacking microRNA 133a develop dynamin 2–dependent centronuclear myopathy. J. Clin. Invest. 121, 3258–3268 [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. [47]Chen W. S., Leung C. M., Pan H. W., Hu L. Y., Li S. C., Ho M. R., Tsai K. W. (2012) Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer. Oncol. Rep. 28, 1069–1076 [DOI] [PubMed] [Google Scholar]
  46. [48]Huang L., Xi Z., Wang C., Zhang Y., Yang Z., Zhang S., Chen Y., Zuo Z. (2016) Phenanthrene exposure induces cardiac hypertrophy via reducing miR-133a expression by DNA methylation. Sci. Rep. 6, 20105 [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. [49]Westerholm R., Christensen A., Törnqvist M., Ehrenberg L., Rannug U., Sjögren M., Rafter J., Soontjens C., Almén J., Gränn K. (2001) Comparison of exhaust emissions from Swedish Environmental classified diesel fuel (MK1) and European Program on Emissions, Fuels and Engine Technologies (EPEFE) reference fuel: a chemical and biological characterization, with viewpoints on cancer risk. Environ. Sci. Technol. 35, 1748–1754 [DOI] [PubMed] [Google Scholar]
  48. [50]Gilsbach R., Preissl S., Grüning B. A., Schnick T., Burger L., Benes V., Würch A., Bönisch U., Günther S., Backofen R., Fleischmann B. K., Schübeler D., Hein L. (2014) Dynamic DNA methylation orchestrates cardiomyocyte development, maturation and disease. Nat. Commun. 5, 5288 [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. [51]Wauters A., Dreyfuss C., Pochet S., Hendrick P., Berkenboom G., van de Borne P., Argacha J. F. (2013) Acute exposure to diesel exhaust impairs nitric oxide-mediated endothelial vasomotor function by increasing endothelial oxidative stress. Hypertension 62, 352–358 [DOI] [PubMed] [Google Scholar]
  50. [52]Zuo L., Youtz D. J., Wold L. E. (2011) Particulate matter exposure exacerbates high glucose-induced cardiomyocyte dysfunction through ROS generation. PLoS One 6, e23116 [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. [53]Valinluck V., Tsai H. H., Rogstad D. K., Burdzy A., Bird A., Sowers L. C. (2004) Oxidative damage to methyl-CpG sequences inhibits the binding of the methyl-CpG binding domain (MBD) of methyl-CpG binding protein 2 (MeCP2). Nucleic Acids Res. 32, 4100–4108 [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. [54]Hackett J. A., Sengupta R., Zylicz J. J., Murakami K., Lee C., Down T. A., Surani M. A. (2013) Germline DNA demethylation dynamics and imprint erasure through 5-hydroxymethylcytosine. Science 339, 448–452 [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. [55]Guo J. U., Su Y., Zhong C., Ming G. L., Song H. (2011) Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain. Cell 145, 423–434 [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. [56]Brenet F., Moh M., Funk P., Feierstein E., Viale A. J., Socci N. D., Scandura J. M. (2011) DNA methylation of the first exon is tightly linked to transcriptional silencing. PLoS One 6, e14524 [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. [57]García R., Villar A. V., Cobo M., Llano M., Martín-Durán R., Hurlé M. A., Nistal J. F. (2013) Circulating levels of miR-133a predict the regression potential of left ventricular hypertrophy after valve replacement surgery in patients with aortic stenosis. J. Am. Heart Assoc. 2, e000211 [DOI] [PMC free article] [PubMed] [Google Scholar]
  56. [58]Dakhlallah D., Zhang J., Yu L., Marsh C. B., Angelos M. G., Khan M. (2015) MicroRNA-133a engineered mesenchymal stem cells augment cardiac function and cell survival in the infarct heart. J. Cardiovasc. Pharmacol. 65, 241–251 [DOI] [PMC free article] [PubMed] [Google Scholar]

Cited by

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Authors
Goodson, J. M., Weldy, C. S., MacDonald, J. W., Liu, Y., Bammler, T. K., Chien, W., & Chin, M. T.
Affiliations
University of Washington (US)
Corresponding author
Chin, Michael T.
ORCID
Venue
The FASEB Journal
Publisher
Wiley
Published
2017-07-27
Volume
31
Issue
11
Pages
4935-4945
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
DOI
10.1096/fj.201700032r
PMC
PMC5636696
OpenAlex
W2740338359
Cited by
25
References
2
Citations by year
2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 5 · 2020: 4 · 2019: 5 · 2018: 5
FWCI
2.06
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • The FASEB Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Washington
  • National Institutes of Health
  • National Institute of Environmental Health Sciences (T32 ES007032, P30ES07033)
  • National Heart, Lung, and Blood Institute (T32 HL007312)
  • American Heart Association (15GRNT25390012)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
Keywords
air pollution, transverse aortic constriction, DNA methylation, PTPRF, cardiomyocytes, epigenetics, cardiac gene expression
MeSH terms
Animals, Aortic Diseases, Vehicle Emissions, Female, Gene Expression Regulation, Male, Myocardium, Pregnancy, Prenatal Exposure Delayed Effects, Serine Endopeptidases, Transcription, Genetic, Maternal Exposure, DNA Methylation, MicroRNAs, Mice, Receptor-Like Protein Tyrosine Phosphatases, Class 2, Serine Proteases

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