Intrinsically Disordered Regions Define Unique Protein Interaction Networks in CHD Family Remodelers
Open accessPublished version of a preprint
Abstract
Chromodomain helicase DNA‐binding (CHD) enzymes play a pivotal role in genome regulation. They possess highly conserved ATPase domains flanked by poorly characterized and intrinsically disordered N‐ and C‐termini. Using mass spectrometry, we identify dozens of novel protein–protein interactions (PPIs) within the N‐ and C‐termini of human CHD family members. We also define a highly conserved aggregation‐prone region (APR) within the C‐terminus of CHD4 which is critical for its interaction with the nucleosome remodeling and deacetylase (NuRD), as well as ChAHP (CHD4, activity‐dependent neuroprotective protein (ADNP), and HP1γ) complexes. Further analysis reveals a regulatory role for the CHD4 APR in gene transcription during erythrocyte formation. Our results highlight that the N‐ and C‐termini of CHD chromatin remodelers shape protein interaction networks that drive unique transcriptional programs.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · see citing works
Accurate structure prediction of biomolecular interactions with AlphaFold 3
Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., et al. (2024) · Nature · doi:10.1038/s41586-024-07487-w · Cited by 15,861 · see citing works
AlphaFold and Implications for Intrinsically Disordered Proteins
Ruff, K. M., & Pappu, R. V. (2021) · Journal of Molecular Biology · doi:10.1016/j.jmb.2021.167208 · Cited by 635 · see citing works
Understanding nucleosome dynamics and their links to gene expression and DNA replication
Lai, W. K. M., & Pugh, B. F. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.47 · Cited by 495 · see citing works
From interaction networks to interfaces, scanning intrinsically disordered regions using AlphaFold2
Bret, H., Gao, J., Zea, D. J., Andreani, J., & Guerois, R. (2024) · Nature Communications · doi:10.1038/s41467-023-44288-7 · Cited by 103 · see citing works
Flagged first, then by citations
- Authors
- Tabar, M. S., Parsania, C., Giardina, C., Feng, Y., Wong, A. C. H., Metierre, C., Nagarajah, R., Dhungel, B. P., Rasko, J. E. J., & Bailey, C. G.
- Affiliations
- The University of Sydney (AU)
- Royal Prince Alfred Hospital (AU)
- Corresponding author
- Rasko, John E. J.
- Bailey, Charles G.
- ORCID
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2025-05-15
- Volume
- 39
- Issue
- 10
- Pages
- e70632-e70632
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- PMC
- PMC12080455
- OpenAlex
- W4410400478
- Cited by
- 7
- References
- 5
- Citations by year
- 2026: 6 · 2025: 1
- FWCI
- 2.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The FASEB Journal (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Health and Medical Research Council (#1177305, #1128748)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- CHD4, intrinsically disordered regions, protein-protein interaction, protein termini, aggregation-prone regions, NuRD complex, ADNP, HP1γ, chromatin remodeling, gene transcription, erythroid differentiation, genome regulation
- MeSH terms
- DNA Helicases, DNA-Binding Proteins, Humans, Chromatin Assembly and Disassembly, Mi-2 Nucleosome Remodeling and Deacetylase Complex, Protein Interaction Maps, Intrinsically Disordered Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
