Levels of tau phosphorylation at different sites in Alzheimer disease brain
Abstract
The microtubule-associated protein tau is abnormally hyperphosphorylated in Alzheimer's disease (AD) brain. To date, 21 phosphorylated sites of tau have been identified. In the present study the levels of phosphorylation at Ser199/Ser202, Thr231/Ser235, Ser262/Ser356 and Ser396/Ser404 of tau in AD brain homogenate and its 100,000 x g supernatant were determined using radioimmuno-dot-blot assay. In homogenate, Ser199/Ser202 and Ser262/Ser356 were phosphorylated to similar level and were more phosphorylated than Thr231 or Ser396/Ser404. In supernatant, there was no significant difference in phosphorylated tau level among the investigated sites except for Thr231/Ser235 which was least phosphorylated. These results suggest that Ser199/Ser202 and Ser262/Ser356 are major sites of phosphorylation of tau in AD brain.
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Tau phosphorylation at S202/T205 as detected by the AT8 antibody is commonly found in tauopathies.
“Tau phosphorylation at S202/T205 as detected by the AT8 antibody (80) is commonly found in tauopathies (38,81–84).”
From Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation (2011) · cites this paper as [83]
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Ambegaokar, S. S., & Jackson, G. R. (2011) · Human Molecular Genetics · doi:10.1093/hmg/ddr432 · Cited by 113 · see citing works
- Authors
- Ikura, Y., Kudo, T., Tanaka, T., Tanii, H., Grundke-Iqbal, I., Iqbal, K., & Takeda, M.
- Affiliations
- Osaka University Hospital (JP)
- The University of Osaka (JP)
- Venue
- NeuroReport
- Published
- 1998-07
- Volume
- 9
- Issue
- 10
- Pages
- 2375-2379
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W2065525116
- Cited by
- 31
- References
- 0
- Citations by year
- 2026: 2 · 2025: 3 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2017: 1 · 2015: 1 · 2012: 2
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroreport (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- tau protein, tau phosphorylation, Alzheimer's disease, phosphorylation sites, microtubule-associated proteins, brain homogenate, subcellular fractionation
- MeSH terms
- Aged, Alzheimer Disease, Amino Acid Sequence, Brain, Brain Chemistry, Female, Humans, Male, Molecular Sequence Data, Phosphorylation, Radioimmunoassay, Temporal Lobe, tau Proteins
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