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Cell Loss in Isolated Human Islets Occurs by Apoptosis
Abstract
Purified islet allografts have largely failed to maintain long-term glucose homeostasis in human recipients, and the reasons for this are unclear. It is noteworthy, however, that islet isolation destroys or removes cellular and noncellular elements of the pancreas that could play an important role in supporting islet survival. The purpose of this study was to determine whether human islet isolation leads to the induction of programmed cell death. Human islets were enzymatically isolated from cadaveric donor pancreata using Liberase or Collagenase P, purified over a discontinuous BSA gradient, then cultured in RPMI 1640 at 37 degrees C in 5% CO2 for < or = 7 days. Islets were examined daily by routine histology and immunocytochemistry for islet hormones, DNA fragmentation [cell death; enzyme-linked immunosorbent assay (ELISA) and TUNEL assay] and for transglutaminase (TG) activity, two indicators of apoptosis. TG activity and DNA fragmentation increased by 1,000% and 1,890%, respectively (p < 0.05) This corresponded to the appearance of pyknotic nuclei on light microscopy, the presence of apoptotic bodies on electron microscopy, and the demonstration of TUNEL-positive cells. These were present primarily in a distribution that corresponded to the insulin-immunoreactive cells. At 5 days, 31.4 +/- 2.2% of islet cells were TUNEL positive. In summary, apoptosis of islet cells appears soon after islet isolation, and involves primarily the beta cell. This is the first report of apopt…
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“Furthermore, the stress of purification from the pancreas can damage islets before transplantation.27”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [27]
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Cites
Regressive Events in Neurogenesis
Cowan, W. M., Fawcett, J. W., O'Leary, D. D. M., & Stanfield, B. B. (1984) · Science · doi:10.1126/science.6474175 · Cited by 1,316
Research works printed but not linked: 33
- Alejandro, 1986, J Clin Invest, 78, 1339, 10.1172/JCI112720 · DOI
- Davalli, 1995, Transplantation, 59, 817, 10.1097/00007890-199503000-00003 · DOI
- Lanza, 1992, Diabetes, 41, 1503, 10.2337/diab.41.12.1503 · DOI
- Hammonds, 1990, FEBS Lett, 261, 97, 10.1016/0014-5793(90)80645-Y · DOI
- Kerr, 1973, Virchows Arch B Cell Pathol, 13, 87, 10.1007/BF02889300 · DOI
- Wyllie, 1973, J Pathol, 111, 85, 10.1002/path.1711110203 · DOI
- Petrik, 1998, Endocrinology, 139, 2994, 10.1210/endo.139.6.6042 · DOI
- Meredith, 1993, Mol Biol Cell, 4, 953, 10.1091/mbc.4.9.953 · DOI
- Frisch, 1994, J Cell Biol, 124, 619, 10.1083/jcb.124.4.619 · DOI
- Wang, 1999, J Histochem Cytochem, 47, 499, 10.1177/002215549904700408 · DOI
- Ricordi, 1988, Diabetes, 37, 413, 10.2337/diab.37.4.413 · DOI
- Gomis, 1983, Biochim Biophys Acta, 760, 384, 10.1016/0304-4165(83)90378-1 · DOI
- Lowry, 1951, J Biol Chem, 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
- Downs, 1983, Anal Biochem, 131, 538, 10.1016/0003-2697(83)90212-9 · DOI
- Sutherland, 1995, Diabetes Metab Rev, 11, 337, 10.1002/dmr.5610110404 · DOI
- Scaglia, 1995, Endocrinology, 136, 5461, 10.1210/endo.136.12.7588296 · DOI
- Blume, 1995, J Clin Invest, 96, 2227, 10.1172/JCI118278 · DOI
- Kerr, 1972, Br J Cancer, 26, 239, 10.1038/bjc.1972.33 · DOI
- Steller, 1995, Science, 267, 1445, 10.1126/science.7878463 · DOI
- Boudreau, 1995, Science, 267, 891, 10.1126/science.7531366 · DOI
- Raff, 1992, Nature, 356, 397, 10.1038/356397a0 · DOI
- Folk, 1980, Annu Rev Biochem, 49, 517, 10.1146/annurev.bi.49.070180.002505 · DOI
- Fesus, 1987, FEBS Lett, 224, 104, 10.1016/0014-5793(87)80430-1 · DOI
- Fesus, 1991, Eur J Cell Biol, 56, 170
- Fesus, 1989, FEBS Lett, 245, 150, 10.1016/0014-5793(89)80210-8 · DOI
- Knight, 1991, Biochim Biophys Acta, 1096, 312, 10.1016/0925-4439(91)90067-J · DOI
- Piacentini, 1991, Eur J Cell Biol, 54, 246
- Thomazy, 1989, Cell Tissue Res, 255, 215, 10.1007/BF00229084 · DOI
- Piacentini, 1995, Curr Top Microbiol Immunol, 200, 163
- Wyllie, 1986, J Pathol, 148, 94A
- Dykes, 1987, Clin Res, 35, 139A
- Finegood, 1995, Diabetes, 44, 249, 10.2337/diab.44.3.249 · DOI
- Davalli, 1996, Diabetes, 45, 1161, 10.2337/diab.45.9.1161 · DOI
Cited by
Sorted by citations
Response of Human Islets to Isolation Stress and the Effect of Antioxidant Treatment
Bottino, R., Balamurugan, A. N., Tse, H. M., Thirunavukkarasu, C., Ge, X., Profozich, J., Milton, M. J., Ziegenfuss, A., Trucco, M., & Piganelli, J. D. (2004) · Diabetes · doi:10.2337/diabetes.53.10.2559 · Cited by 277
“New data have shown that islet function is already undermined by stressful events before transplantation (6,7).”
Zhang, N., Richter, A. M., Suriawinata, J., Harbaran, S., Altomonte, J., Cong, L., Zhang, H., Song, K., Meseck, M. L., Bromberg, J. S., et al. (2004) · Diabetes · doi:10.2337/diabetes.53.4.963 · Cited by 233
“In addition, there is evidence that islet cells undergo apoptosis because of ischemia-reperfusion injury or oxidative stress in islet isolation, contributing to the loss of functional islet mass during the peri-transplant period (30,31).”
Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes
Hogrebe, N. J., Ishahak, M., & Millman, J. R. (2023) · Cell Stem Cell · doi:10.1016/j.stem.2023.04.002 · Cited by 210
Pancreatic islet transplantation
Corrêa‐Giannella, M. L., & do Amaral, A. S. R. (2009) · Diabetology & Metabolic Syndrome · doi:10.1186/1758-5996-1-9 · Cited by 28
“Thus, the process of islets isolation results in pro-inflammatory and oxidative stress states, with loss of cellular viability and apoptosis [31] which begin soon after the isolation and are closely associated to the failure of the islet transplant [32].”
BackgroundCentral Necrosis in Isolated Hypoxic Human Pancreatic Islets: Evidence for Postisolation Ischemia
Giuliani, M., Moritz, W., Bodmer, E., Dindo, D. R., Kugelmeier, P., Lehmann, R., Gassmann, M., Groscurth, P., & Weber, M. (2005) · Cell Transplantation · doi:10.3727/000000005783983287 · Cited by 170
Metadata
- Authors
- Paraskevas, S., Maysinger, D., Wang, R., Duguid, W. P., & Rosenberg, L.
- Affiliations
- Montreal General Hospital (CA)
- Corresponding author
- Rosenberg, Lawrence
- ORCID
- Paraskevas, Steven
- Venue
- Pancreas
- Published
- 2000-04
- Volume
- 20
- Issue
- 3
- Pages
- 270-276
- ISSN
- 0885-3177 (ISSN-L)
- OpenAlex
- W2046236577
- Cited by
- 229
- References
- 0
- Citations by year
- 2024: 6 · 2023: 7 · 2022: 6 · 2021: 8 · 2020: 7 · 2019: 7 · 2018: 15 · 2017: 10 · 2016: 11 · 2015: 9 · 2014: 7 · 2013: 14 · 2012: 12
- FWCI
- 7.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Pancreas (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- islet isolation, apoptosis, beta-cell death, human pancreatic islets, islet transplantation, transglutaminase activity, DNA fragmentation, TUNEL, Liberase, islet purification
- MeSH terms
- Adolescent, Adult, Cadaver, Cell Separation, Enzyme-Linked Immunosorbent Assay, Humans, Immunohistochemistry, Islets of Langerhans, Microscopy, Electron, Middle Aged, Transglutaminases, Thermolysin, Apoptosis, Collagenases, In Situ Nick-End Labeling, DNA Fragmentation
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