The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Clinical Utility of Polymerase Chain Reaction Testing for Streptococcus pneumoniae in Pediatric Cerebrospinal Fluid Samples: A Diagnostic Accuracy Study of More Than 2000 Samples From 2004 to 2015
Abstract
The aim of this retrospective study was to review the diagnostic accuracy of real-time polymerase chain reaction (PCR) testing of cerebrospinal fluid (CSF) samples for Streptococcus pneumoniae DNA in comparison with traditional bacterial culture. The hypothesis was that PCR is more sensitive than culture and would detect more cases of pneumococcal meningitis, particularly in children treated with antimicrobials before CSF sampling occurred. Patients younger than 16 years of age who had a CSF sample tested for S. pneumoniae DNA by PCR between 2004 and 2015 were included. A total of 2025 samples were included, and the PCR had a sensitivity of 100% and specificity of 98% for the detection of S. pneumoniae DNA in comparison with culture. Of the 28 culture negative/PCR positive cases, 25 (89%) were probable meningitis cases and only 3 (11%) were suspected false positive results. Nineteen (76%) of the 25 probable cases required ICU admission, and 3 died (12%). Six different serotypes were found in the culture positive patients (18C, 6B, 14, 22F, 7F and 33F). This study demonstrates that PCR testing of CSF samples for S. pneumoniae is sensitive and specific when compared with culture. PCR is particularly useful in detecting those cases where culture is negative, perhaps relating to pre-CSF sampling administration of antimicrobials.
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“The reported sensitivity of PCR for pneumococcal meningitis is high and reached 100% in the majority of conducted studies (median of 100%, range 79%–100%) (Table 1; see also Table S1 in the supplemental material) (49–58).”
From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [50]
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Pneumococcal carriage in young children after introduction of PCV13 in Hong Kong
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Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections
Olie, S. E., Andersen, C. Ø., van de Beek, D., & Brouwer, M. C. (2024) · Clinical Microbiology Reviews · doi:10.1128/cmr.00021-24 · Cited by 38
“Several studies found that PCR for S. pneumoniae provided additional diagnostic value in culture-negative bacterial meningitis (49, 50, 53, 55).”
Metadata
- Authors
- Alqayoudhi, A., Nielsen, M., O’Sullivan, N., Corcoran, M., Gavin, P. J., Butler, K. M., Cunney, R., & Drew, R. J.
- Affiliations
- Royal College of Surgeons in Ireland (IE)
- Corresponding author
- Alqayoudhi, Abdullah
- Gavin, Patrick J.
- Butler, Karina M.
- Drew, Richard J.
- Venue
- Pediatric Infectious Disease Journal
- Published
- 2017-09
- Volume
- 36
- Issue
- 9
- Pages
- 833-836
- ISSN
- 0891-3668 (ISSN-L)
- OpenAlex
- W2605387217
- Cited by
- 7
- References
- 16
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 2
- FWCI
- 0.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Pediatric Infectious Disease Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
- Keywords
- Streptococcus pneumoniae, pneumococcal meningitis, cerebrospinal fluid, real-time PCR, bacterial culture, diagnostic accuracy, pediatric patients, false positives, serotypes
- MeSH terms
- Data Accuracy, Bacteriological Techniques, Child, Child, Preschool, DNA, Bacterial, Female, Humans, Infant, Male, Medical Audit, Meningitis, Pneumococcal, Retrospective Studies, Streptococcus pneumoniae, Polymerase Chain Reaction, Molecular Diagnostic Techniques
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