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Cited by 6Gynecological conditions and treatmentsTissue Engineering and Regenerative MedicineMesenchymal stem cell research

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Transplantation of acellular amniotic membrane seeded with adipose-derived mesenchymal stem cells in a rat model of intrauterine adhesion

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Li, Chunbo, Yao, Liangfeng, He, Fengquan, Hua, Keqin·2024·Annals of Medicine & Surgery
DOI 10.1097/ms9.0000000000002249PMC PMC11305806
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Abstract

Objective: This study aimed to investigate the role of acellular amniotic membrane (AAM) loaded with adipose-derived mesenchymal stem cells (ADSCs) for the treatment of intrauterine adhesion. Methods: One hundred twenty female Spargue-Dawley rats were randomly divided into four groups: sham operation group (the uterus was picked out and incised without treatment), intrauterine adhesion group, the experimental group treated with AAM, and experimental group treated with AAM loaded with ADSCs. Histological and immunohistochemical analysis were performed on 3, 7, and 14 days after surgery to evaluate the degree of uterine fibrosis and regeneration of injured endometrium. RNA sequencing and real-time PCR were used to explore the potential mechanism by which ADSCs modulated immune response and promoted endometrial regeneration. Results: On 14 days after surgery, the endometrial thickness, number of glands, and degree of fibrosis reduction in the ADSCs/AAM group was higher than those in the AAM group, and similar to the sham operation group. RNA sequencing analysis showed that ADSCs can modulate local immune responses and promote the formation of functional endometrium. Meanwhile, we found that ADSCs significantly decreased the levels of pro-inflammatory cytokines (TNF-α and IL-1β) and increased the levels of anti-inflammatory cytokines (bFGF and IL-6). Conclusion: Our results demonstrated that AAM loaded with ADSCs can result in the regeneration of injured endometrium and fibrosis reduction. Meanwhile, ADSCs also regulated the immune microenvironment, which was beneficial to functional endometrial recovery.

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  • Rats receiving ADSC-seeded amnion grafts showed near-normal uterine recovery: by 14 days post-surgery, endometrial thickness, gland count, and fibrosis scores approached those of uninjured sham animals and were significantly better than AM alone.

    “Rats receiving ADSC-seeded amnion grafts showed near-normal uterine recovery: by 14 days post-surgery, endometrial thickness, gland count, and fibrosis scores in the ADSC + AM group approached those of uninjured sham animals and were significantly better than AM alone [116].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [116]

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  • Despite improved histology, fertility outcomes remained suboptimal in the ADSC-seeded amnion rat model; high miscarriage rates persisted even with ADSC treatment.

    “Despite improved histology, fertility outcomes remained suboptimal in this model; high miscarriage rates persisted even with ADSC treatment, possibly due to residual uterine receptivity issues [116].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [116]

  • ADSCs on the scaffold modulated the injury site immune environment by lowering pro-inflammatory TNF-α and IL-1β and increasing anti-inflammatory factors bFGF and IL-6, promoting a regenerative response.

    “Mechanistically, the ADSCs on the scaffold modulated the injury site immune environment—lowering pro-inflammatory TNF-α and IL-1β, and increasing anti-inflammatory factors (bFGF, IL-6)—promoting a regenerative response.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [116]

  • Residual uterine receptivity issues possibly caused the persistent high miscarriage rates in the ADSC-seeded amnion rat model.

    “high miscarriage rates persisted even with ADSC treatment, possibly due to residual uterine receptivity issues [116].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [116]

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Research works printed but not linked: 35

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Metadata

Authors
Li, C., Yao, L., He, F., & Hua, K.
Affiliations
  • Shanghai Medical College of Fudan University (CN)
  • Zhangzhou Municipal Hospital of Fujian Province (CN)
  • Department of Obstetrics and Gynecology, Maternal and Child Health Hospital of HongHe Hani and Yi Autonomous Prefecture, Yunnan, China
Corresponding author
  • He, Fengquan
  • Hua, Keqin
ORCID
Venue
Annals of Medicine & Surgery
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2024-07-01
Volume
86
Issue
8
Pages
4463-4474
ISSN
2049-0801 (ISSN-L)
DOI
10.1097/ms9.0000000000002249
PMC
PMC11305806
OpenAlex
W4400120598
Cited by
6
References
1
Citations by year
2026: 3 · 2025: 3
FWCI
2.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Annals of Medicine and Surgery (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Obstetrics and Gynecology › Gynecological conditions and treatments
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
Keywords
intrauterine adhesions, acellular amniotic membrane, adipose-derived stem cells, cell transplantation, endometrial regeneration, uterine fibrosis, endometrial injury, immune microenvironment, inflammatory cytokines, anti-inflammatory cytokines, rat model

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