The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Fungal denitrification and nitric oxide reductase cytochrome P450nor
Open accessAbstract
We have shown that many fungi (eukaryotes) exhibit distinct denitrifying activities, although occurrence of denitrification was previously thought to be restricted to bacteria (prokaryotes), and have characterized the fungal denitrification system. It comprises NirK (copper-containing nitrite reductase) and P450nor (a cytochrome P450 nitric oxide (NO) reductase (Nor)) to reduce nitrite to nitrous oxide (N(2)O). The system is localized in mitochondria functioning during anaerobic respiration. Some fungal systems further contain and use dissimilatory and assimilatory nitrate reductases to denitrify nitrate. Phylogenetic analysis of nirK genes showed that the fungal-denitrifying system has the same ancestor as the bacterial counterpart and suggested a possibility of its proto-mitochondrial origin. By contrast, fungi that have acquired a P450 from bacteria by horizontal transfer of the gene, modulated its function to give a Nor activity replacing the original Nor with P450nor. P450nor receives electrons directly from nicotinamide adenine dinucleotide to reduce NO to N(2)O. The mechanism of this unprecedented electron transfer has been extensively studied and thoroughly elucidated. Fungal denitrification is often accompanied by a unique phenomenon, co-denitrification, in which a hybrid N(2) or N(2)O species is formed upon the combination of nitrogen atoms of nitrite with a nitrogen donor (amines and imines). Possible involvement of NirK and P450nor is suggested.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Importantly, multiple fungal species, including the ubiquitous Candida, contain a fungal nitrate reductase enzyme and are known nitrate-reducers in the environment[229,230].”
From Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health (2017) · cites this paper as [230]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cell biology and molecular basis of denitrification
Zumft, W. G. (1997) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.61.4.533-616.1997 · Cited by 3,560
Missing lithotroph identified as new planctomycete
Strous, M., Fuerst, J. A., Kramer, E. H. M., Logemann, S., Gerard Muyzer, C., van de Pas-Schoonen, K. T., Webb, R. I., Kuenen, J. G., & Jetten, M. S. M. (1999) · Nature · doi:10.1038/22749 · Cited by 1,578
Berks, B. C., Ferguson, S. J., Moir, J. W. B., & Richardson, D. J. (1995) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(95)00092-5 · Cited by 596
Dissimilatory Nitrite and Nitric Oxide Reductases
Averill, B. A. (1996) · Chemical Reviews · doi:10.1021/cr950056p · Cited by 545
Evidence for Fungal Dominance of Denitrification and Codenitrification in a Grassland Soil
Laughlin, R. J., & Stevens, R. J. (2002) · Soil Science Society of America Journal · doi:10.2136/sssaj2002.1540 · Cited by 425
Shoun, H., Kim, D., Uchiyama, H., & Sugiyama, J. (1992) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1992.tb05331.x · Cited by 422
Mitochondria as we don't know them
Tielens, A. G. M., Rotte, C., van Hellemond, J. J., & Martin, W. F. (2002) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(02)02193-x · Cited by 375
Shoun, H., & Tanimoto, T. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99130-1 · Cited by 254
Nakahara, K., Tanimoto, T., Hatano, K., Usuda, K., & Shoun, H. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53102-1 · Cited by 249
Spectroscopic and Kinetic Studies on Reaction of Cytochrome P450nor with Nitric Oxide
Shiro, Y., Fujii, M., Iizuka, T., Adachi, S., Tsukamoto, K., Nakahara, K., & Shoun, H. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.4.1617 · Cited by 205
Cited by
Sorted by citations
The microbial nitrogen-cycling network
Kuypers, M. M. M., Marchant, H. K., & Kartal, B. (2018) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2018.9 · Cited by 4,563
Hu, H., Chen, D., & He, J. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv021 · Cited by 825
“to N2O (Shoun et al. 2012).”
Genomics and Ecology of Novel N2O-Reducing Microorganisms
Hallin, S., Philippot, L., Löffler, F. E., Sanford, R. A., & Jones, C. M. (2017) · Trends in Microbiology · doi:10.1016/j.tim.2017.07.003 · Cited by 655
Fungal traits that drive ecosystem dynamics on land
Treseder, K. K., & Lennon, J. T. (2015) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00001-15 · Cited by 625
Role of organic amendment application on greenhouse gas emission from soil
Thangarajan, R., Bolan, N. S., Tian, G., Naidu, R., & Kunhikrishnan, A. (2013) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2013.01.031 · Cited by 533
“In general, more than 60 genera of bacteria, Archaea, fungi, and yeast can perform denitrification (Cabello et al., 2004; Philippot et al., 2007; Shoun et al., 2012; Watanabe et al., 2009) with most soil denitrifiers falling under four major genera: Pseudomonas, Bacillus, Alcaligenes, and Flavobacterium (Tiedje, 1988).”
Background
Metadata
- Authors
- Shoun, H., Fushinobu, S., Jiang, L., Kim, S.-W., & Wakagi, T.
- Affiliations
- The University of Tokyo (JP)
- Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
- Venue
- Philosophical Transactions of the Royal Society B Biological Sciences
- Publisher
- The Royal Society
- Published
- 2012-05-05
- Volume
- 367
- Issue
- 1593
- Pages
- 1186-1194
- ISSN
- 0962-8436 (ISSN-L)
- 1471-2970
- PMC
- PMC3306627
- OpenAlex
- W2127344845
- Cited by
- 256
- References
- 50
- Citations by year
- 2026: 17 · 2025: 14 · 2024: 22 · 2023: 20 · 2022: 20 · 2021: 24 · 2020: 17 · 2019: 23 · 2018: 19 · 2017: 14 · 2016: 26 · 2015: 15 · 2014: 10 · 2013: 13 · 2012: 2
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Philosophical Transactions of the Royal Society B Biological Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Tokyo
- University of Tsukuba
- Chiba University
- Universität Konstanz
- Japan Society for the Promotion of Science (20248009)
- Topics
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- fungal denitrification, nitric oxide reductase, nirK, nitrite reductase, nitrous oxide, anaerobic respiration, mitochondrial electron transport chain, horizontal gene transfer, phylogenetic analysis, nitrate reductase
- MeSH terms
- Cytochrome P-450 Enzyme System, Fungi, Models, Molecular, Nitric Oxide, Nitrite Reductases, Nitrous Oxide, Denitrification
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
