The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Differentiation of Mycobacterium species by direct sequencing of amplified DNA
Abstract
Nucleotide sequences specific for a range of Mycobacterium species were defined by computer-assisted sequence comparisons of small subunit ribosomal RNA. A polymerase chain reaction-based sequencing strategy was used to demonstrate that the 16S rRNA sequence can be used for the rapid identification of mycobacterial isolates. Identification at the species level can be obtained within 2 d, requiring less than 10,000 bacteria. This procedure reliably differentiates Mycobacterium spp. which are difficult to identify by classical methods, such as M. malmoense, M. szulgai and M. flavescens.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
Springer, B., Stockman, L., Teschner, K., Roberts, G. D., & Böttger, E. C. (1996) · Journal of Clinical Microbiology · doi:10.1128/jcm.34.2.296-303.1996 · Cited by 371
Metadata
- Authors
- Rogall, T., Flohr, T., & Bottger, E. C.
- Venue
- Journal of General Microbiology
- Publisher
- Microbiology Society
- Published
- 1990-09-01
- Volume
- 136
- Issue
- 9
- Pages
- 1915-1920
- ISSN
- 0022-1287 (ISSN-L)
- OpenAlex
- W2129074874
- Cited by
- 298
- References
- 1
- FWCI
- 13.99
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Differentiation of Mycobacterium species by direct sequencing of amplified DNA. https://app.cochat.ai/papers/10.1099/00221287-136-9-1915
- The paper
- Rogall, T., Flohr, T., & Bottger, E. C. (1990). Differentiation of Mycobacterium species by direct sequencing of amplified DNA. Journal of General Microbiology. https://doi.org/10.1099/00221287-136-9-1915
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
