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Temperature regulation of protease in Pseudomonas fluorescens LS107d2 by an ECF sigma factor and a transmembrane activator The GenBank accession numbers for the sequences reported in this paper are AF228766 and AF228767.

Burger, Michelle, Woods, Rick G., McCarthy, Conor, Beacham, Ifor R.·2000·Microbiology
DOI 10.1099/00221287-146-12-3149
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Abstract

The production of extracellular enzymes by Pseudomonas fluorescens is important with respect to phytopathogenesis and, in the case of psychrotrophic strains, food spoilage. The production of extracellular protease has been previously reported to be dependent on temperature in psychrotrophic strains of P. fluorescens; production is decreased above the optimum growth temperature with a relatively small change in growth rate. In this work, a transposon mutant of P. fluorescens LS107d2 has been isolated which, in contrast to the wild-type strain, is completely protease deficient at 29 degrees C, above the optimum growth temperature of 25 degrees C, but which produces protease at 23 degrees C. Further analysis revealed that this mutation is in a gene (prtR) which is part of a dicistronic operon, prtIR, in which the two genes are translationally coupled. Evidence is presented that prtI encodes a sigma factor related to others involved in extracytoplasmic functions (ECF sigma factors) and that prtR encodes a novel transmembrane activator of PrtI. PrtI, like PrtR, is also required for protease production at 29 degrees C but not at 23 degrees C. Analysis of the amino acid sequence of PrtR indicates that it is functionally related to a group of membrane-associated anti-sigma factors and a few transmembrane regulators, but is not significantly sequence related. Complementation analysis indicates that PrtR may also interact with sigma factors other than PrtI. The promoter region of the p…

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  • PrtR is annotated as a transmembrane σ factor and has been previously described in P. fluorescens to control the formation of extracellular proteases and siderophores, as well as temperature-dependent lipases and proteases.

    “PrtR is annotated as a transmembrane σ factor, and it has been previously described to display a role in P. fluorescens in controlling the formation of extracellular proteases and siderophores, as well as temperature‐dependent lipases and proteases (Burger et al., 2000; Llamas et al., 2014; Song et al., 2014).”

    From Role of the CrcB transporter of Pseudomonas putida in the multi‐level stress response elicited by mineral fluoride (2022) · cites this paper as Burger et al., 2000

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Metadata

Authors
Burger, M., Woods, R. G., McCarthy, C., & Beacham, I. R.
Affiliations
School of Health Science, Griffith University, PMB 50 Gold Coast Mail Centre, Gold Coast, Qld 4217, Australia1
Venue
Microbiology
Publisher
Microbiology Society
Published
2000-12-01
Volume
146
Issue
12
Pages
3149-3155
ISSN
  • 1350-0872
  • 1465-2080
DOI
10.1099/00221287-146-12-3149
Cited by
48
References
0
Language
en

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