The DOI matches its Crossref record.
Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes The EMBL accession numbers for the sequences reported in this paper are AJ245436 [P. putida (oleovorans) GPo1 alk gene clusters and flanking DNA], AJ233397 (P. putida P1 alk gene clusters and flanking DNA), AJ249793 (P. putida P1 nahKJ genes), AJ249825 [P. putida (oleovorans) GPo1 16S RNA gene] and AJ271219 (P. putida P1 16S RNA gene).
Abstract
The Pseudomonas putida GPo1 (commonly known as Pseudomonas oleovorans GPo1) alkBFGHJKL and alkST gene clusters, which encode proteins involved in the conversion of n-alkanes to fatty acids, are located end to end on the OCT plasmid, separated by 9.7 kb of DNA. This DNA segment encodes, amongst others, a methyl-accepting transducer protein (AlkN) that may be involved in chemotaxis to alkanes. In P. putida P1, the alkBFGHJKL and alkST gene clusters are flanked by almost identical copies of the insertion sequence ISPpu4, constituting a class 1 transposon. Other insertion sequences flank and interrupt the alk genes in both strains. Apart from the coding regions of the GPo1 and P1 alk genes (80-92% sequence identity), only the alkB and alkS promoter regions are conserved. Competition experiments suggest that highly conserved inverted repeats in the alkB and alkS promoter regions bind ALKS:
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Citations
Cited by
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Feng, L., Wang, W., Cheng, J., Ren, Y., Zhao, G., Gao, C., Tang, Y., Liu, X., Han, W., Peng, X., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609650104 · Cited by 396
Diversity of Alkane Hydroxylase Systems in the Environment
Van Beilen, J. B., Li, Z., Duetz, W. A., Smits, T. H. M., & Witholt, B. (2003) · Oil & Gas Science and Technology · doi:10.2516/ogst:2003026 · Cited by 375
Self-citationDiverse alkane hydroxylase genes in microorganisms and environments
Nie, Y., Chi, C.-Q., Fang, H., Liang, J.-L., Lu, S.-L., Lai, G.-L., Tang, Y.-Q., & Wu, X.-L. (2014) · Scientific Reports · doi:10.1038/srep04968 · Cited by 261
Medić, A., Lješević, M., Inui, H., Beškoski, V., Kojić, I., Stojanović, K., & Karadžić, I. (2020) · RSC Advances · doi:10.1039/c9ra10371f · Cited by 129
Metadata
- Authors
- van Beilen, J. B., Panke, S., Lucchini, S., Franchini, A. G., Röthlisberger, M., & Witholt, B.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2001-06-01
- Volume
- 147
- Issue
- 6
- Pages
- 1621-1630
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W1709457880
- Cited by
- 326
- References
- 0
- FWCI
- 15.99
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes The EMBL accession numbers for the sequences reported in this paper are AJ245436 [P. putida (oleovorans) GPo1 alk gene clusters and flanking DNA], AJ233397 (P. putida P1 alk gene clusters and flanking DNA), AJ249793 (P. putida P1 nahKJ genes), AJ249825 [P. putida (oleovorans) GPo1 16S RNA gene] and AJ271219 (P. putida P1 16S RNA gene). https://app.cochat.ai/papers/10.1099/00221287-147-6-1621
- The paper
- van Beilen, J. B., Panke, S., Lucchini, S., Franchini, A. G., Röthlisberger, M., & Witholt, B. (2001). Analysis of Pseudomonas putida alkane-degradation gene clusters and flanking insertion sequences: evolution and regulation of the alk genes The EMBL accession numbers for the sequences reported in this paper are AJ245436 [P. putida (oleovorans) GPo1 alk gene clusters and flanking DNA], AJ233397 (P. putida P1 alk gene clusters and flanking DNA), AJ249793 (P. putida P1 nahKJ genes), AJ249825 [P. putida (oleovorans) GPo1 16S RNA gene] and AJ271219 (P. putida P1 16S RNA gene). Microbiology. https://doi.org/10.1099/00221287-147-6-1621
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
