The DOI matches its Crossref record; 41 of the 42 references it cites were found in a registry or an index.
Characterization of a novel Vibrio pathogenicity island (VPI-2) encoding neuraminidase (nanH) among toxigenic Vibrio cholerae isolates
Abstract
Acquisition of virulence genes encoded on mobile genetic elements has played an important role in the emergence of pathogenic isolates of Vibrio cholerae, the causative agent of the diarrhoeal disease cholera. The genes encoding cholera toxin (ctxAB), the main cause of profuse secretory diarrhoea in cholera, are encoded on a filamentous bacteriophage CTXphi. The toxin coregulated pilus (TCP), an essential intestinal colonization factor, was originally designated as part of a pathogenicity island named the Vibrio pathogenicity island (VPI), but this island has more recently been proposed to be the genome of a filamentous phage, VPIphi. In this study, it is shown that nanH, which encodes neuraminidase, maps within a novel pathogenicity island designated VPI-2. The 57.3 kb VPI-2 has all of the characteristic features of a pathogenicity island, including the presence of a bacteriophage-like integrase (int), insertion in a tRNA gene (serine) and the presence of direct repeats at the chromosomal integration sites. Additionally, the G+C content of VPI-2 (42 mol%) is considerably lower than that of the entire genome (47 mol%). VPI-2 encodes several gene clusters, such as a restriction modification system (hsdR and hsdM) and genes required for the utilization of amino sugars (nan-nag region) as well as neuraminidase. To determine the distribution of VPI-2 among V. cholerae, 78 natural isolates were examined using PCR and Southern hybridization analysis for the presence of this region. All toxigenic V. cholerae O1 serogroup isolates examined contained VPI-2, whereas non-toxigenic isolates lacked the island. Of 14 V. cholerae O139 serogroup isolates examined, only one strain, MO2, contained the entire 57.3 kb island, whereas 13 O139 isolates contained only a 20.0 kb region with most of the 5' region of VPI-2 which included nanH deleted in these strains.
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“NA is encoded within a pathogenicity island of the Vibrio cholerae genome [30].”
From Characterization of Vibrio cholerae neuraminidase as an immunomodulator for novel formulation of oral allergy immunotherapy (2018) · cites this paper as [30]
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Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion
Brüssow, H., Canchaya, C. A., & Hardt, W. (2004) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.68.3.560-602.2004 · Cited by 1,717
Pathogenicity Islands in BacterialPathogenesis
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Boyd, E. F., & Brüssow, H. (2002) · Trends in Microbiology · doi:10.1016/s0966-842x(02)02459-9 · Cited by 381
Self-citationAre pathogenic bacteria just looking for food? Metabolism and microbial pathogenesis
Rohmer, L., Hocquet, D., & Miller, S. I. (2011) · Trends in Microbiology · doi:10.1016/j.tim.2011.04.003 · Cited by 364
Dziejman, M., Serruto, D., Tam, V. C., Sturtevant, D., Diraphat, P., Faruque, S. M., Rahman, M., Heidelberg, J. F., Decker, J., Li, L., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409918102 · Cited by 221
Metadata
- Authors
- Jermyn, W. S., & Boyd, E. F.
- Affiliations
- National University of Ireland (IE)
- Corresponding author
- Boyd, E. Fidelma
- ORCID
- Boyd, E. Fidelma
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2002-11-01
- Volume
- 148
- Issue
- 11
- Pages
- 3681-3693
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W1841852894
- Cited by
- 198
- References
- 42
- Citations by year
- 2025: 13 · 2024: 17 · 2023: 12 · 2022: 17 · 2021: 8 · 2020: 9 · 2019: 4 · 2018: 10 · 2017: 2 · 2016: 6 · 2015: 8 · 2014: 13 · 2013: 6 · 2012: 12
- FWCI
- 4.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Enterprise Ireland
- Higher Education Authority
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- Vibrio cholerae, neuraminidase, nanH, virulence genes, mobile genetic elements, toxin-coregulated pilus, cholera toxin, O139 serogroup, O1 serogroup, restriction-modification systems, gene clusters, GC content
- MeSH terms
- Humans, Neuraminidase, Statistics as Topic, Vibrio cholerae, Polymerase Chain Reaction, Virulence Factors
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