The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
A PCR-based strategy to generate integrative targeting alleles with large regions of homology
Abstract
Cryptococcus neoformans is an opportunistic fungal pathogen with a defined sexual cycle for which genetic and molecular techniques are well developed. The entire genome sequence of one C. neoformans strain is nearing completion. The efficient use of this sequence is dependent upon the development of methods to perform more rapid genetic analysis including gene-disruption techniques. A modified PCR overlap technique to generate targeting constructs for gene disruption that contain large regions of gene homology is described. This technique was used to disrupt or delete more than a dozen genes with efficiencies comparable to those previously reported using cloning technology to generate targeting constructs. Moreover, it is shown that disruptions can be made using this technique in a variety of strain backgrounds, including the pathogenic serotype A isolate H99 and recently characterized stable diploid strains. In combination with the availability of the complete genomic sequence, this gene-disruption technique should pave the way for higher throughput genetic analysis of this important pathogenic fungus.
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Cites
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Noble, S. M., & Johnson, A. D. (2005) · Eukaryotic Cell · doi:10.1128/ec.4.2.298-309.2005 · Cited by 592
Light Controls Growth and Development via a Conserved Pathway in the Fungal Kingdom
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Metadata
- Authors
- Davidson, R. C., Blankenship, J. R., Kraus, P. R., de Jesus Berrios, M., Hull, C. M., D’Souza, C., Wang, P., & Heitman, J.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2002-08-01
- Volume
- 148
- Issue
- 8
- Pages
- 2607-2615
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2137549147
- Cited by
- 299
- References
- 47
- FWCI
- 7.13
- Type
- journal article
- Language
- en
- Open access
- closed
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- Davidson, R. C., Blankenship, J. R., Kraus, P. R., de Jesus Berrios, M., Hull, C. M., D’Souza, C., Wang, P., & Heitman, J. (2002). A PCR-based strategy to generate integrative targeting alleles with large regions of homology. Microbiology. https://doi.org/10.1099/00221287-148-8-2607
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