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A Fractionation Procedure for Studies of the Synthesis of Cell-Wall Mucopeptide and of Other Polymers in Cells of Staphylococcus aureus
Abstract
SUMMARY: A method is described for simultaneous estimation of pool constituents, protein, nucleic acids, cell-wall mucopeptide, and possibly teichoic acid in a single sample of S. aureus. The method makes possible isolation of the cell-wall mucopeptide contaminated by less than 5% of the cytoplasmic protein. It is suitable for studies of biosynthesis, with radioactive precursors, and requires approximately 3 mg. cell/sample.
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Citations
Cited by
5 Analysis of the Chemical Composition and Primary Structure of Murein
Schleifer, K. H. (1985) · Methods in Microbiology · doi:10.1016/s0580-9517(08)70474-4 · Cited by 710
Wise, E. M., & Park, J. T. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.54.1.75 · Cited by 347
Self-citationLocalization of Protein A in the Bacteria
Sjöquist, J., Movitz, J., Johansson, I., & Hjelm, H. (1972) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1972.tb02086.x · Cited by 150
The N,O-Diacetylmuramidase of Chalaropsis Species
Hash, J. H., & Rothlauf, M. V. (1967) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99397-x · Cited by 104
Bacteriolytic Action of Fluoride Ions
Lesher, R. J., Bender, G. R., & Marquis, R. E. (1977) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.12.3.339 · Cited by 40
Metadata
- Authors
- PARK, J. T., & HANCOCK, R.
- Venue
- Journal of General Microbiology
- Publisher
- Microbiology Society
- Published
- 1960-02-01
- Volume
- 22
- Issue
- 1
- Pages
- 249-258
- ISSN
- 0022-1287 (ISSN-L)
- OpenAlex
- W2130283290
- Cited by
- 270
- References
- 0
- FWCI
- 51.16
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A Fractionation Procedure for Studies of the Synthesis of Cell-Wall Mucopeptide and of Other Polymers in Cells of Staphylococcus aureus. https://app.cochat.ai/papers/10.1099/00221287-22-1-249
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- PARK, J. T., & HANCOCK, R. (1960). A Fractionation Procedure for Studies of the Synthesis of Cell-Wall Mucopeptide and of Other Polymers in Cells of Staphylococcus aureus. Journal of General Microbiology. https://doi.org/10.1099/00221287-22-1-249
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
