The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
A Defined Medium for Mycoplasma Strain Y
Abstract
SUMMARY In a defined medium for Mycoplasma strain Y, a mixture of the diacetoxysuccinoyl esters of monoolein and monopalmitin replaced unesterified fatty acids and serum protein fractions, enabling the minimal growth requirements to be determined. Other Mycoplasma strains did not grow in media in which these esters replaced fatty acids and protein fractions.
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“However, M. mycoides capri LC Y has been found capable of taking up all amino acids in their free form (Rodwell, 1969) (glutamic and aspartic acid not investigated); thus, the least constraining assumption is made that both permeases can take up all amino acids, except for glutamic and aspartic acid, whose uptake is already enabled by GltP.”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Thiouridine tRNA modification through IscS and MnmA (iscS/0441 and mnmA/0387) appears to be non-essential in M. mycoides capri LC Y, which can grow without a precursor for the IscS cofactor pyridoxal phosphate (Rodwell, 1969).”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“We note that the absence of related salvage enzymes in JCVI-syn3A necessitates the uptake of complete coenzyme A; this is in accordance with the experimental requirement of M. mycoides capri LC Y for coenzyme A rather than coenzyme A precursors (Rodwell, 1967; Rodwell, 1969), as well as the apparently incomplete coenzyme A salvage pathway already in JCVI-syn1.0.”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
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In the citing paper
“the minimal media for both M. mycoides capri LC Y (Rodwell, 1969) and M. pneumoniae (Yus et al., 2009) hence include spermine”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
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“While free lipoate is a component of the minimal media for M. mycoides capri LC Y (Rodwell, 1969) and M. pneumoniae (Yus et al., 2009) and is a possible ECF system substrate (Rodionov et al., 2009), two putative lipoyl transferases have been deleted in JCVI-syn3A (MMSYN1_0224 and MMSYN1_0464), such that free lipoate cannot be used to provide the lipoyl moiety of pyruvate dehydrogenase subunit E2 (”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
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In the citing paper
“the in vivo quasi-essentiality of the glutamate/aspartate permease GltP is surprising in the light of M. mycoides capri LC Y reportedly not depending on these two amino acids being provided in the media (Rodwell, 1969)”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
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“The quasi-essentiality of Opp (and, possibly functionally related, three peptidases) is consistent with M. mycoides capri LC Y requiring alanine in peptide form for optimal growth (Rodwell, 1969); the growth rate decreases a bit if instead only free alanine is provided.”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
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In the citing paper
“The quasi-essentiality of Opp suggests that this effect could be more pronounced in the SP4 media, which might not have free alanine at the 1 mM concentration used in Rodwell (1969).”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
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In the citing paper
“experimental observations of folate-free growth (Rodwell, 1969; Neale et al., 1981)”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“The uptake requirement of M. mycoides capri for some form of thymine (Rodwell, 1969; Neale et al., 1983a), in spite of availability of dUMP, indicates the absence of thymidylate synthase activity (an otherwise common usage for dUMP as a precursor for dTMP), in line with the lack of an annotation for a thymidylate synthase gene in JCVI-syn3A.”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
Experimental — accuracy not yet measuredThe check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“nucleic acid stabilization by polyamines is a known essential process”
From Essential metabolism for a minimal cell (2019) · cites this paper as Rodwell, 1969
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Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
Presence of the Arginine Dihydrolase Pathway in Mycoplasma
Barile, M. F., SCHIMKE, R. T., & Riggs, D. B. (1966) · Journal of Bacteriology · doi:10.1128/jb.91.1.189-192.1966 · Cited by 95
NUTRITION AND METABOLISM OF MYCOPLASMA MYCOIDES VAR. MYCOIDES
Rodwell, A. W. (1960) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1960.tb42716.x · Cited by 56
Self-citationThe nutrition and metabolism of mycoplasma: progress and problems
Rodwell, A. W. (1967) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1967.tb27649.x · Cited by 53
Self-citationA PLEUROPNEUMONIA‐LIKE ORGANISM CAUSING PERITONITIS IN GOATS.
Laws, L. (1956) · Australian Veterinary Journal · doi:10.1111/j.1751-0813.1956.tb08243.x · Cited by 45
707. Glyceride synthesis by direct esterification
HARTMAN, L. (1957) · Journal of the Chemical Society (Resumed) · doi:10.1039/jr9570003572 · Cited by 38
The Steroid Growth-Requirement of Mycoplasma mycoides
Rodwell, A. W. (1963) · Journal of General Microbiology · doi:10.1099/00221287-32-1-91 · Cited by 32
Self-citationThe Supply of Cholesterol and Fatty Acids for the Growth of Mycoplasmas
Rodwell, A. W. (1969) · Journal of General Microbiology · doi:10.1099/00221287-58-1-29 · Cited by 31
Self-citationGrowth of Mycoplasma gallisepticum Strain J Without Serum.
LUND, P. G., & Shore, M. S. (1966) · Experimental Biology and Medicine · doi:10.3181/00379727-121-30969 · Cited by 14
Synthesis of a Homologous Series of Optically Active Normal Aliphatic α-Monoglycerides (L-Series)
Baer, E., & Fischer, H. O. L. (1945) · Journal of the American Chemical Society · doi:10.1021/ja01227a049
Cited by
Sorted by citations
MOLECULAR BIOLOGY OF MYCOPLASMAS
Dybvig, K. F., & Voelker, L. L. (1996) · Annual Review of Microbiology · doi:10.1146/annurev.micro.50.1.25 · Cited by 231
CULTURE MEDIA FOR CLASSIC MYCOPLASMAS
Freundt, E. A. (1983) · Elsevier eBooks · doi:10.1016/b978-0-12-583801-6.50029-9 · Cited by 209
Essential metabolism for a minimal cell
Breuer, M., Earnest, E. E., Merryman, C., Wise, K. S., Sun, L., Lynott, M. R., Hutchison, C. A., Smith, H. O., Lapek, J. D., Gonzalez, D. J., et al. (2019) · eLife · doi:10.7554/elife.36842 · Cited by 190
“mycoides capri LC Y for coenzyme A rather than coenzyme A precursors (Rodwell, 1967; Rodwell, 1969), as well as the apparently incomplete coenzyme A salvage pathway already in JCVIsyn1.”
BackgroundResultInfluentialA genome-scale metabolic reconstruction of Mycoplasma genitalium, iPS189
Suthers, P. F., Dasika, M. S., Kumar, V. S., Denisov, G., Glass, J. I., & Maranas, C. D. (2009) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000285 · Cited by 144
Nicholas, R. A. J., & Bashiruddin, J. B. (1995) · Journal of Comparative Pathology · doi:10.1016/s0021-9975(05)80065-9 · Cited by 62
Metadata
- Authors
- RODWELL, A. W.
- Corresponding author
- RODWELL, A. W.
- Venue
- Journal of General Microbiology
- Publisher
- Microbiology Society
- Published
- 1969-09-01
- Volume
- 58
- Issue
- 1
- Pages
- 39-47
- ISSN
- 0022-1287 (ISSN-L)
- OpenAlex
- W2109023235
- Semantic Scholar
- 6f76659b1735483aaa85eb821530b9fc3d5fe2dd
- Cited by
- 28
- References
- 6
- Citations by year
- 2022: 1 · 2021: 1 · 2019: 1 · 2017: 2 · 2015: 1
- FWCI
- 2.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytoplasmas and Hemiptera pathogens
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- defined culture medium, monoolein, monopalmitin
- MeSH terms
- Alanine, Amino Acids, Culture Media, Esters, Glycerides, Glycerol, Thioctic Acid, Mycoplasma, Niacinamide, Palmitic Acids, Riboflavin, Succinates, Thiamine
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