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Cited by 431

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

An Electron-microscopic Demonstration of an Acidic Polysaccharide Involved in the Adhesion of a Marine Bacterium to Solid Surfaces

FLETCHER, M., FLOODGATE, G. D.·1973·Journal of General Microbiology
DOI 10.1099/00221287-74-2-325
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Abstract

SUMMARY: Staining a marine bacterium with Ruthenium Red and Alcian Blue demonstrated an extracellular, compact acidic polysaccharide layer, which was involved in bacterial adhesion to surfaces. The adhesive substance was present on suspended bacteria and appeared to assist adhesion when they were forced into contact with a suitable surface. Bacteria, which had attached to a surface naturally, produced a secondary fibrous acidic polysaccharide, which probably developed from the primary polysaccharide, and could eventually replace it. A high pH in the growth medium almost totally prevented the appearance of primary polysaccharide in preparations of naturally attached bacteria, which were surrounded by the reticular secondary polysaccharide, and adhesion was not impaired. In contrast to naturally attached bacteria, those forcibly attached were surrounded by primary polysaccharide. High temperature lowered the number of bacteria attached, relative to culture density, but did not affect the appearance of the adhesive substance.

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Metadata

Authors
FLETCHER, M., & FLOODGATE, G. D.
Venue
Journal of General Microbiology
Publisher
Microbiology Society
Published
1973-02-01
Volume
74
Issue
2
Pages
325-334
ISSN
0022-1287 (ISSN-L)
DOI
10.1099/00221287-74-2-325
OpenAlex
W2144677436
Cited by
431
References
15
FWCI
3.65
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: An Electron-microscopic Demonstration of an Acidic Polysaccharide Involved in the Adhesion of a Marine Bacterium to Solid Surfaces. https://app.cochat.ai/papers/10.1099/00221287-74-2-325
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FLETCHER, M., & FLOODGATE, G. D. (1973). An Electron-microscopic Demonstration of an Acidic Polysaccharide Involved in the Adhesion of a Marine Bacterium to Solid Surfaces. Journal of General Microbiology. https://doi.org/10.1099/00221287-74-2-325

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.