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The Use of Analytical Isoelectric Focusing for Detection and Identification of -Lactamases
Abstract
BETA-Lactamases (EC. 3.5.2.6) from strains of Gram-negative bacteria have been studied using analytical isoelectric focusing. This permits a visual comparison of the patterns of beta-lactamase bands produced by enzymes from different organisms. Purification of crude intracellular preparations is unnecessary and the technique is sufficiently sensitive to demonstrate beta-lactamase in mutants previously reported to lack the enzyme. R that have not been distinguished from one another biochemically or immunologically can be differentiated by isoelectric focusing. Conversely, the enzymes specified by the R factors RTEM, R1 and RGN14, with identical isoelectric focusing patterns have the same biochemical properties. Chromosomal and R-factor-mediated beta-lactamases from single strains have been separated and their identities confirmed by immunoisoelectric focusing. R factor-mediated enzymes gave identical isoelectric focusing patterns irrespective of the host strain. Isoelectric focusing can therefore be used to observe the transfer of beta-lactamases carried by R factors.
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Cited by
Kim, Y.-K., Pai, H., Lee, H.-J., Park, S.-E., Choi, E.-H., Kim, J., Kim, J.-H., & Kim, E.-C. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.5.1481-1491.2002 · Cited by 357
The ABCD’s of β-lactamase nomenclature
Bush, K. (2013) · Journal of Infection and Chemotherapy · doi:10.1007/s10156-013-0640-7 · Cited by 239
Emergence of Candida tropicalis resistant to caspofungin
Pasquale, T., Tomada, J. R., Ghannoun, M., Dipersio, J., & Bonilla, H. (2007) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkm453 · Cited by 56
Metadata
- Authors
- MATTHEW, M., HARRIS, A. M., MARSHALL, M. J., & ROSS, G. W.
- Venue
- Journal of General Microbiology
- Publisher
- Microbiology Society
- Published
- 1975-05-01
- Volume
- 88
- Issue
- 1
- Pages
- 169-178
- ISSN
- 0022-1287 (ISSN-L)
- OpenAlex
- W2002178722
- Cited by
- 688
- References
- 0
- FWCI
- 8.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: The Use of Analytical Isoelectric Focusing for Detection and Identification of -Lactamases. https://app.cochat.ai/papers/10.1099/00221287-88-1-169
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- MATTHEW, M., HARRIS, A. M., MARSHALL, M. J., & ROSS, G. W. (1975). The Use of Analytical Isoelectric Focusing for Detection and Identification of -Lactamases. Journal of General Microbiology. https://doi.org/10.1099/00221287-88-1-169
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