CoChat
Sign Up
Cited by 57Antibiotic Resistance in BacteriaNeonatal and Maternal InfectionsEnterobacteriaceae and Cronobacter Research

The DOI matches its Crossref record.

Extended-spectrum β-lactamase-producing Gram-negative bacteria causing neonatal sepsis in India in rural and urban settings

Open access
Chandel, Dinesh S., Johnson, Judith A., Chaudhry, Rama, Sharma, Nidhi, Shinkre, Nandita, Parida, Sailajanandan, Misra, Pravas R., Panigrahi, Pinaki·2011·Journal of Medical Microbiology
DOI 10.1099/jmm.0.027375-0PMC PMC3133666
Open this paper in CoChat — free account

Abstract

Extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacilli (GNB) are of increasing clinical concern in all age groups worldwide. Whilst sepsis continues to be the leading cause of morbidity and mortality in Indian neonates in the community, identification of microbiological attributes in this population is lacking. This population-based study enrolled 1738 infants with a diagnosis of clinical sepsis at four participating centres in India. Each study site conducted Bactec blood culture, identified bacterial species by API test and stored isolates at −70 °C. From 252 GNB isolates, 155 (113 Klebsiella species, 21 Escherichia coli and 21 other) were subjected to drug susceptibility testing, ESBL phenotyping and testing for clonal relatedness of ESBL strains by PFGE. The results demonstrated that Klebsiella species and E. coli are the most common GNB causes of neonatal sepsis in India, and over one-third are ESBL producers in both community and hospital settings. ESBL-producing strains exhibited frequent co-resistance to aminoglycosides and ciprofloxacin, but remained susceptible to imipenem. PFGE analysis revealed extensive genetic diversity within the ESBL-producing isolates, showing multiple profiles (total of 23). Over 40 % of all ESBL-producing isolates formed three pulsed-field profiles (PFP I–III), with PFP-II being the largest cluster (>20 % of all ESBL-producing isolates), sharing strains from two distant locations. Identification of a common clone at two geographically distant centres indicated that predominant clones with increased virulence may exist, even in the absence of any clear outbreak. The presence of ESBL-producing strains in community infants with no prior history of hospitalization or antibiotic use dictates heightened vigilance and further studies on the ecology of these organisms.

Overview

Integrity signals

Registry

Crossref · 10.1099/jmm.0.027375-0

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The impacts of slum residents' inappropriate antibiotic consumption practices on community-level antimicrobial resistance may explain why a higher proportion of enteric and bloodstream infections among children living in urban informal settlements are resistant to first-line antibiotics compared to children living in rural areas.

    “Overall, the impacts of slum residents' inappropriate antibiotic consumption practices on community-level antimicrobial resistance may explain why a higher proportion of enteric and bloodstream infections among children living in urban informal settlements are resistant to first-line antibiotics, compared to children living in rural areas 27-29 .”

    From Urban informal settlements as hotspots of antimicrobial resistance and the need to curb environmental transmission. (2020) · cites this paper as [27]

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 30

  1. Anandan, 2009, Prevalence of extended-spectrum β -lactamase producing Escherichia coli and Klebsiella spp in a neonatal intensive care unit, Indian Pediatr, 46, 1106
  2. Arpin, 2003, Extended-spectrum β -lactamase-producing Enterobacteriaceae in community and private health care centers, Antimicrob Agents Chemother, 47, 3506, 10.1128/AAC.47.11.3506-3514.2003 · DOI
  3. Bagattini, 2006, Molecular epidemiology of extended-spectrum β -lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit, J Antimicrob Chemother, 57, 979, 10.1093/jac/dkl077 · DOI
  4. Ben-Ami, 2006, Influx of extended-spectrum β -lactamase-producing Enterobacteriaceae into the hospital, Clin Infect Dis, 42, 925, 10.1086/500936 · DOI
  5. Bradford, 2001, Extended-spectrum β -lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat, Clin Microbiol Rev, 14, 933, 10.1128/CMR.14.4.933-951.2001 · DOI
  6. Calbo, 2006, Risk factors for community-onset urinary tract infections due to Escherichia coli harbouring extended-spectrum β -lactamases, J Antimicrob Chemother, 57, 780, 10.1093/jac/dkl035 · DOI
  7. Cartelle, 2004, Risk factors for colonization and infection in a hospital outbreak caused by a strain of Klebsiella pneumoniae with reduced susceptibility to expanded-spectrum cephalosporins, J Clin Microbiol, 42, 4242, 10.1128/JCM.42.9.4242-4249.2004 · DOI
  8. Decré, 1998, Clinical and bacteriologic epidemiology of extended-spectrum β -lactamase-producing strains of Klebsiella pneumoniae in a medical intensive care unit, Clin Infect Dis, 27, 834, 10.1086/514938 · DOI
  9. Dubois, 2010, CTX-M-producing Escherichia coli in a maternity ward: a likely community importation and evidence of mother-to-neonate transmission, J Antimicrob Chemother, 65, 1368, 10.1093/jac/dkq153 · DOI
  10. Jain, 2003, Prevalence of extended-spectrum β -lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital, J Med Microbiol, 52, 421, 10.1099/jmm.0.04966-0 · DOI
  11. Jarlier, 1988, Extended broad-spectrum β -lactamases conferring resistance to newer β -lactam agents in Enterobacteriaceae : hospital prevalence and susceptibility patterns, Rev Infect Dis, 10, 867, 10.1093/clinids/10.4.867 · DOI
  12. Kim, 2002, Bloodstream infections by extended-spectrum β -lactamase-producing Escherichia coli and Klebsiella pneumoniae in children: epidemiology and clinical outcome, Antimicrob Agents Chemother, 46, 1481, 10.1128/AAC.46.5.1481-1491.2002 · DOI
  13. Krishna, 2007, Extended spectrum β lactamase producing Klebsiella pneumoniae in neonatal intensive care unit, Indian J Pediatr, 74, 627, 10.1007/s12098-007-0111-1 · DOI
  14. Lautenbach, 2001, Extended-spectrum β -lactamase-producing Escherichia coli and Klebsiella pneumoniae : risk factors for infection and impact of resistance on outcomes, Clin Infect Dis, 32, 1162, 10.1086/319757 · DOI
  15. Manchanda, 2006, Molecular epidemiology of clinical isolates of AmpC producing Klebsiella pneumoniae, Indian J Med Microbiol, 24, 177, 10.1016/S0255-0857(21)02346-X · DOI
  16. Mirelis, 2003, Community transmission of extended-spectrum β -lactamase, Emerg Infect Dis, 9, 1024, 10.3201/eid0908.030094 · DOI
  17. 1997, Neonatal morbidity and mortality: report of the National Neonatal–Perinatal Database, Indian Pediatr, 34, 1039
  18. 2001, Zone diameter interpretive standards, NCCLS global information supplement, 21, 40
  19. Perilli, 2002, Molecular characterization of extended-spectrum β -lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey, J Clin Microbiol, 40, 611, 10.1128/JCM.40.2.611-614.2002 · DOI
  20. Pitout, 2008, Extended-spectrum β -lactamase-producing Enterobacteriaceae : an emerging public-health concern, Lancet Infect Dis, 8, 159, 10.1016/S1473-3099(08)70041-0 · DOI
  21. Rodrigues, 2005, Extended-spectrum β -lactamase-producing flora in healthy persons, Emerg Infect Dis, 11, 981, 10.3201/eid1106.041111 · DOI
  22. Rodríguez-Baño, 2004, Epidemiology and clinical features of infections caused by extended-spectrum β -lactamase-producing Escherichia coli in non-hospitalized patients, J Clin Microbiol, 42, 1089, 10.1128/JCM.42.3.1089-1094.2004 · DOI
  23. Sahly, 2008, Extended-spectrum β -lactamase production is associated with an increase in cell invasion and expression of fimbrial adhesins in Klebsiella pneumoniae, Antimicrob Agents Chemother, 52, 3029, 10.1128/AAC.00010-08 · DOI
  24. Struelens, 1996, Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems, Clin Microbiol Infect, 2, 2, 10.1111/j.1469-0691.1996.tb00193.x · DOI
  25. Talbot, 2006, Bad bugs need drugs: an update on the development pipeline from the Antimicrobial Availability Task Force of the Infectious Diseases Society of America, Clin Infect Dis, 42, 657, 10.1086/499819 · DOI
  26. Tenover, 1995, Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial isolate typing, J Clin Microbiol, 33, 2233, 10.1128/JCM.33.9.2233-2239.1995 · DOI
  27. Thambu, 2006, Community-acquired, fatal extended spectrum β lactamase producing Klebsiella pneumoniae splenic abscess and sepsis, J Postgrad Med, 52, 328
  28. GroupMathai, 2002, Evaluation of the in vitro activity of six broad-spectrum β -lactam antimicrobial agents tested against recent clinical isolates from India: a survey of ten medical center laboratories, Diagn Microbiol Infect Dis, 44, 367, 10.1016/S0732-8893(02)00466-2 · DOI
  29. Valverde, 2004, Dramatic increase in prevalence of fecal carriage of extended-spectrum β -lactamase-producing Enterobacteriaceae during non-outbreak situations in Spain, J Clin Microbiol, 42, 4769, 10.1128/JCM.42.10.4769-4775.2004 · DOI
  30. Weber, 2003, Predictors of neonatal sepsis in developing countries, Pediatr Infect Dis J, 22, 711, 10.1097/01.inf.0000078163.80807.88 · DOI

Cited by

Sorted by citations

Metadata

Authors
Chandel, D. S., Johnson, J. A., Chaudhry, R., Sharma, N., Shinkre, N., Parida, S., Misra, P. R., & Panigrahi, P.
Affiliations
  • Asian Institute of Public Health (IN)
  • University of Florida (US)
  • All India Institute of Medical Sciences (IN)
  • BYL Nair Hospital and TN Medical College, Mumbai, India
  • Sriram Chandra Bhanja Medical College Hospital (IN)
  • University of Nebraska Medical Center (US)
ORCID
Venue
Journal of Medical Microbiology
Publisher
Microbiology Society
Published
2011-04-01
Volume
60
Issue
4
Pages
500-507
ISSN
  • 0022-2615 (ISSN-L)
  • 1473-5644
DOI
10.1099/jmm.0.027375-0
PMC
PMC3133666
OpenAlex
W2168352858
Cited by
57
References
0
Citations by year
2025: 1 · 2024: 2 · 2023: 10 · 2022: 5 · 2021: 2 · 2020: 2 · 2019: 5 · 2018: 2 · 2017: 5 · 2016: 5 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 2
FWCI
1.19
Type
journal article
Language
en
Open access
Locations
  • Journal of Medical Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Bill and Melinda Gates Foundation
  • Indian Council of Medical Research
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (U01HD040574, HD 40574)
Topics
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and Maternal Infections
  • Life Sciences › Immunology and Microbiology › Microbiology › Enterobacteriaceae and Cronobacter Research
Keywords
extended-spectrum beta-lactamase, neonatal sepsis, Gram-negative bacilli, Klebsiella, Escherichia coli, antimicrobial resistance, aminoglycoside resistance, ciprofloxacin resistance, imipenem susceptibility, molecular epidemiology, pulsed-field gel electrophoresis, clonal relatedness, community-acquired infections, India
MeSH terms
Anti-Bacterial Agents, beta-Lactamases, Genotype, Gram-Negative Bacteria, Humans, India, Infant, Microbial Sensitivity Tests, Rural Population, Urban Population, Genetic Variation, Bacterial Typing Techniques, Electrophoresis, Gel, Pulsed-Field, Gram-Negative Bacterial Infections, Sepsis, Molecular Typing

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review