HEPES Inhibits the Conversion of Prion Protein in Cell Culture
Abstract
HEPES is a well-known buffering reagent used in cell-culture medium. Interestingly, this compound is also responsible for significant modifications of biological parameters such as uptake of organic molecules, alteration of oxidative stress mechanisms or inhibition of ion channels. While using cell-culture medium supplemented with HEPES on prion-infected cells, it was noticed that there was a significant concentration-dependent inhibition of accumulation of the abnormal isoform of the prion protein (PrP(Sc)). This effect was present only in live cells and was thought to be related to modification of the PrP environment or biology. These results could modify the interpretation of cell-culture assays of prion therapeutic agents, as well as of previous cell biology results obtained in the field using HEPES buffers. This inhibitory effect of HEPES could also be exploited to prevent contamination or propagation of prions in cell culture.
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HEPES activated lysosomal transcription factors in macrophages, inhibited the prion protein conversion in neural stem cells and affected their viability and differentiation.
“Briefly, HEPES activated lysosomal transcription factors in macrophages (Tol et al., 2018), inhibited the prion protein conversion in neural stem cells and affected their viability and differentiation (Delmouly et al., 2011).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Delmouly et al., 2011
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References 38 · Cited by 5
- Authors
- Delmouly, K., Belondrade, M., Casanova, D., Milhavet, O., & Lehmann, S.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Institut de Génétique Humaine, CNRS-UPR 1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 2011-05-01
- Volume
- 92
- Issue
- 5
- Pages
- 1244-1250
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2169362407
- Cited by
- 5
- References
- 38
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2018: 1 · 2015: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Virology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- National Science Council
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Prion Diseases and Protein Misfolding
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
- Keywords
- HEPES, prion protein, PrPSc, prion conversion, cell culture media, prion propagation
- MeSH terms
- Culture Media, Enzyme Inhibitors, HEPES, Prions, Protein Folding, Cell Culture Techniques
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