The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
Open accessAbstract
The ability to detect an infectious agent in a widespread epidemic is crucial to the success of quarantine efforts in addition to sensitive and accurate screening of potential cases of infection from patients in a clinical setting. Enabling testing outside of sophisticated laboratories broadens the scope of control and surveillance efforts, but also requires robust and simple methods that can be used without expensive instrumentation. Here we report a method to identify SARS-CoV-2 (COVID-19) virus RNA from purified RNA or cell lysis using loop-mediated isothermal amplification (LAMP) using a visual, colorimetric detection. This test was additionally verified using RNA samples purified from respiratory swabs collected from COVID-19 patients in Wuhan, China with equivalent performance to a commercial RT-qPCR test while requiring only heating and visual inspection. This simple and sensitive method provides an opportunity to facilitate virus detection in the field without a requirement for complex diagnostic infrastructure.
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Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Huang, C., Wang, Y., Li, X., Ren, L., Zhao, J., Hu, Y., Zhang, L., Fan, G., Xu, J., Gu, X., et al. (2020) · The Lancet · doi:10.1016/s0140-6736(20)30183-5 · Cited by 48,884
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Zhou, P., Yang, X.-L., Wang, X.-G., Hu, B., Zhang, L., Zhang, W., Si, H.-R., Zhu, Y., Li, B., Huang, C.-L., et al. (2020) · Nature · doi:10.1038/s41586-020-2012-7 · Cited by 21,969
Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
Consistent Detection of 2019 Novel Coronavirus in Saliva
To, K. K.-W., Tsang, O. T.-Y., Yip, C. C.-Y., Chan, K.-H., Wu, T.-C., Chan, J. M.-C., Leung, W.-S., Chik, T. S.-H., Choi, C. Y.-C., Kandamby, D. H., et al. (2020) · Clinical Infectious Diseases · doi:10.1093/cid/ciaa149 · Cited by 1,873
Tomita, N., Mori, Y., Kanda, H., & Notomi, T. (2008) · Nature Protocols · doi:10.1038/nprot.2008.57 · Cited by 1,867
Goto, M., Honda, E., Ogura, A., Nomoto, A., & Hanaki, K.-I. (2009) · BioTechniques · doi:10.2144/000113072 · Cited by 1,063
Chen, L., Liu, W., Zhang, Q., Xu, K., Ye, G., Wu, W., Sun, Z., Liu, F., Wu, K., Zhong, B., et al. (2020) · Emerging Microbes & Infections · doi:10.1080/22221751.2020.1725399 · Cited by 570
Calvert, A. E., Biggerstaff, B. J., Tanner, N. A., Lauterbach, M., & Lanciotti, R. S. (2017) · PLoS ONE · doi:10.1371/journal.pone.0185340 · Cited by 106
Self-citationRubinfien, J., Atabay, K. D., Nichols, N. M., Tanner, N. A., Pezza, J. A., Gray, M. M., Wagner, B. M., Poppin, J. N., Aken, J. T., Gleason, E. J., et al. (2019) · FASEB BioAdvances · doi:10.1096/fba.2019-00088 · Cited by 25
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Diagnosing COVID-19: The Disease and Tools for Detection
Udugama, B., Kadhiresan, P., Kozlowski, H. N., Malekjahani, A., Osborne, M., Li, V. Y. C., Chen, H., Mubareka, S., Gubbay, J. B., & Chan, W. C. W. (2020) · ACS Nano · doi:10.1021/acsnano.0c02624 · Cited by 1,754
Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection
Dinnes, J., Deeks, J. J., Adriano, A., Berhane, S., Davenport, C., Dittrich, S., Emperador, D., Takwoingi, Y., Cunningham, J., Beese, S., et al. (2020) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd013705 · Cited by 1,156
Assay Techniques and Test Development for COVID-19 Diagnosis
Carter, L. J., Garner, L. V., Smoot, J. W., Li, Y., Zhou, Q., Saveson, C. J., Sasso, J. M., Gregg, A. C., Soares, D. J., Beskid, T. R., et al. (2020) · ACS Central Science · doi:10.1021/acscentsci.0c00501 · Cited by 985
Rapid, point-of-care antigen tests for diagnosis of SARS-CoV-2 infection
Dinnes, J., Deeks, J. J., Berhane, S., Taylor, M., Adriano, A., Davenport, C., Dittrich, S., Emperador, D., Takwoingi, Y., Cunningham, J., et al. (2021) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd013705.pub2 · Cited by 591
Torrente-Rodríguez, R. M., Lukas, H., Tu, J., Min, J., Yang, Y., Xu, C., Rossiter, H. B., & Gao, W. (2020) · Matter · doi:10.1016/j.matt.2020.09.027 · Cited by 490
Metadata
- Authors
- Zhang, Y., Odiwuor, N., Xiong, J., Sun, L., Nyaruaba, R. O., Wei, H., & Tanner, N. A.
- Venue
- medRxiv
- Publisher
- openRxiv
- Published
- 2020-02-29
- OpenAlex
- W3010323736
- Cited by
- 355
- References
- 9
- Type
- posted content
- Language
- en
- Open access
- green
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- Zhang, Y., Odiwuor, N., Xiong, J., Sun, L., Nyaruaba, R. O., Wei, H., & Tanner, N. A. (2020). Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP. medRxiv. https://doi.org/10.1101/2020.02.26.20028373
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