CoChat
Sign Up
PreprintCited by 16Neuroblastoma Research and TreatmentsNeurofibromatosis and Schwannoma CasesDevelopmental Biology and Gene Regulation

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

TWIST1 expression is associated with high-risk Neuroblastoma and promotes Primary and Metastatic Tumor Growth

Open access
Sepporta, Maria-Vittoria, Praz, Viviane, Bourloud, Katia Balmas, Joseph, Jean-Marc, Jauquier, Nicolas, Riggi, Nicolo’, Nardou-Auderset, Katya, Petit, Audrey, Scoazec, Jean-Yves, Sartelet, Hervé, Renella, Raffaele, Mühlethaler-Mottet, Annick·2021·Communications Biology
DOI 10.1101/2021.03.22.435811PMC PMC8755726

Preprint — published as 10.1038/s42003-021-02958-6 · Paper page

Open this paper in CoChat — free accountOpen PDF

Abstract

The embryonic transcription factors TWIST1/2 are frequently overexpressed in cancer, acting as multifunctional oncogenes. Here we investigate their role in neuroblastoma (NB), a heterogeneous childhood malignancy ranging from spontaneous regression to dismal outcomes despite multimodal therapy. We first reveal the association of TWIST1 expression with poor survival and metastasis in primary NB, while TWIST2 correlates with good prognosis. Secondly, suppression of TWIST1 by CRISPR/Cas9 results in a reduction of tumor growth and metastasis colonization in immunocompromised mice. Moreover, TWIST1 knockout tumors display a less aggressive cellular morphology and a reduced disruption of the extracellular matrix (ECM) reticulin network. Additionally, we identify a TWIST1-mediated transcriptional program associated with dismal outcome in NB and involved in the control of pathways mainly linked to the signaling, migration, adhesion, the organization of the ECM, and the tumor cells versus tumor stroma crosstalk. Taken together, our findings suggest TWIST1 as novel therapeutic target in NB.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2021.03.22.435811

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 59

  1. 10.1056/NEJMra0804577 · DOI
  2. 10.1038/nrc1014 · DOI
  3. 10.1007/s00441-018-2820-3 · DOI
  4. 10.1038/nrc3526 · DOI
  5. 10.1200/jco.2008.16.0630 · DOI
  6. 10.1038/nature14980 · DOI
  7. 2018, A mechanistic classification of clinical phenotypes in neuroblastoma, Science (New York, NY), 362, 1165, 10.1126/science.aat6768 · DOI
  8. 10.1038/nrc3679 · DOI · Paper page
  9. Le Douarin N , LeDouarin NM , Kalcheim C. The neural crest. Cambridge university press, 1999. · DOI
  10. 10.1038/onc.2010.92 · DOI
  11. 2017, Epithelial-mesenchymal transition in tumor metastasis, Molecular oncology, 11, 28, 10.1002/1878-0261.12017 · DOI
  12. 10.1093/nar/gkq890 · DOI
  13. 10.1016/j.cell.2004.06.006 · DOI
  14. 10.1016/j.ccr.2004.09.033 · DOI
  15. 10.3892/ijo.2012.1684 · DOI
  16. 10.1016/j.canlet.2014.11.056 · DOI
  17. 10.1038/s41588-018-0044-9 · DOI
  18. 1977, Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines, Cancer research, 37, 1364
  19. 10.1093/jnci/57.3.683 · DOI
  20. 10.1593/neo.11800 · DOI
  21. 10.1002/ijc.20681 · DOI
  22. 10.1038/nbt.1511 · DOI · Paper page
  23. 10.1021/pr101065j · DOI
  24. 2014, Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ, Molecular & cellular proteomics: MCP, 13, 2513, 10.1074/mcp.M113.031591 · DOI · Paper page
  25. 10.1016/j.ccr.2011.01.036 · DOI
  26. 10.1242/jcs.116392 · DOI · Paper page
  27. 10.1038/s41568-019-0238-1 · DOI · Paper page
  28. 10.1016/s0070-2153(01)51003-0 · DOI
  29. 10.18632/oncotarget.20645 · DOI
  30. 10.1097/FJC.0b013e3182116e39 · DOI
  31. 2002, Reactive stroma in human prostate cancer: induction of myofibroblast phenotype and extracellular matrix remodeling, Clinical cancer research: an official journal of the American Association for Cancer Research, 8, 2912
  32. 10.1016/j.celrep.2016.04.084 · DOI · Paper page
  33. 10.1016/j.ccr.2008.06.005 · DOI
  34. 10.18632/oncotarget.14608 · DOI
  35. 10.1038/onc.2011.181 · DOI
  36. 10.18632/oncotarget.390 · DOI
  37. 10.3892/ol.2013.1316 · DOI
  38. 10.3748/wjg.v19.i15.2404 · DOI
  39. 10.1016/j.stem.2014.12.002 · DOI
  40. 10.1016/j.ccr.2014.01.028 · DOI
  41. 10.1158/0008-5472.CAN-05-0712 · DOI
  42. 10.1073/pnas.1618091114 · DOI
  43. 10.1038/cr.2010.118 · DOI
  44. 10.1016/j.ydbio.2016.08.016 · DOI
  45. 10.1101/gad.9.6.686 · DOI
  46. 10.1038/s41388-018-0482-y · DOI
  47. 10.1038/ncb1010-924 · DOI
  48. 10.1016/j.celrep.2014.12.032 · DOI
  49. 10.1016/j.cell.2008.03.027 · DOI
  50. 2020, Cancer progression and the invisible phase of metastatic colonization, Nature reviews Cancer, 20, 681, 10.1038/s41568-020-00300-6 · DOI
  51. 2020, Concepts of extracellular matrix remodelling in tumour progression and metastasis, Nature communications, 11, 5120, 10.1038/s41467-020-18794-x · DOI · Paper page
  52. 10.1038/bjc.2016.210 · DOI
  53. 10.1016/j.ydbio.2010.08.021 · DOI · Paper page
  54. 10.1038/onc.2016.57 · DOI
  55. 10.1242/bio.019703 · DOI
  56. 10.7150/thno.34090 · DOI
  57. 10.1158/2159-8290.CD-12-0418 · DOI
  58. 2008, Lung metastases in neuroblastoma at initial diagnosis: A report from the International Neuroblastoma Risk Group (INRG) project, Pediatric blood & cancer, 51, 589, 10.1002/pbc.21684 · DOI
  59. 10.1016/j.tranon.2019.04.020 · DOI

Metadata

Authors
Sepporta, M.-V., Praz, V., Bourloud, K. B., Joseph, J.-M., Jauquier, N., Riggi, N., Nardou-Auderset, K., Petit, A., Scoazec, J.-Y., Sartelet, H., Renella, R., & Mühlethaler-Mottet, A.
Affiliations
  • Centre Hospitalier Universitaire Vaudois (CH)
  • Hôpital de la Timone (FR)
  • Institut Gustave Roussy (FR)
  • Centre Hospitalier Régional et Universitaire de Nancy (FR)
ORCID
Venue
Communications Biology
Publisher
openRxiv
Published
2021-03-22
Volume
5
Issue
1
Pages
42-42
ISSN
2399-3642 (ISSN-L)
DOI
10.1101/2021.03.22.435811
PMC
PMC8755726
OpenAlex
W4220760725
Cited by
16
References
1
Citations by year
2026: 6 · 2025: 6 · 2024: 2 · 2023: 1 · 2022: 1
FWCI
1.32
Type
journal article
Language
en
Open access
Licence
Locations
  • Communications Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • SERVAL (Université de Lausanne) (publishedVersion, cc-by)
  • IRIS (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • SERVAL (Université de Lausanne) (submittedVersion, other-oa)
Funders
  • Novartis Foundation
  • École Polytechnique Fédérale de Lausanne
  • Novartis Stiftung für Medizinisch-Biologische Forschung
  • Université de Lausanne
Topics
  • Health Sciences › Medicine › Oncology › Neuroblastoma Research and Treatments
  • Health Sciences › Medicine › Clinical Neurology › Neurofibromatosis and Schwannoma Cases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
TWIST1, TWIST2, neuroblastoma, high-risk neuroblastoma, metastasis, tumor growth, embryonic transcription factors, oncogenes, prognosis, survival, extracellular matrix, tumor-stroma interactions, cell adhesion and migration, therapeutic target, CRISPR/Cas9 knockout
MeSH terms
Animals, Neuroblastoma, Nuclear Proteins, Gene Expression, Gene Expression Regulation, Neoplastic, Immunocompromised Host, Mice, Twist-Related Protein 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review