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PreprintCited by 0Advanced NMR Techniques and ApplicationsMicrobial Natural Products and BiosynthesisMetabolomics and Mass Spectrometry Studies

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NMR as a readout to monitor and restore the integrity of complex chemoenzymatic reactions

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Marincin, Kenneth A., Hwang, Yousang, Kengmana, Everett S., Meyers, David J., Frueh, Dominique P.·2022·Journal of Magnetic Resonance
DOI 10.1101/2022.05.14.491371PMC PMC9463103

Preprint — published as 10.1016/j.jmr.2022.107265 · Paper page

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Abstract

The non-invasive nature of NMR offers a means to monitor biochemical reactions in situ at the atomic-level. We harness this advantage to monitor a complex chemoenzymatic reaction that sequentially modifies reagents and loads the product on a nonribosomal peptide synthetase carrier protein. We present a protocol including a novel pulse sequence that permits to assess both the integrity of reagents and the completion of each step in the reaction, thus alleviating otherwise time-consuming and costly approaches to debug and repeat inefficient reactions. This study highlights the importance of NMR as a tool to establish reliable and reproducible experimental conditions in biochemical studies. Graphical Abstract:

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Metadata

Authors
Marincin, K. A., Hwang, Y., Kengmana, E. S., Meyers, D. J., & Frueh, D. P.
Affiliations
Johns Hopkins University (US)
Corresponding author
Frueh, Dominique P.
ORCID
Venue
Journal of Magnetic Resonance
Publisher
openRxiv
Published
2022-05-14
Volume
342
Pages
107265-107265
ISSN
1090-7807 (ISSN-L)
DOI
10.1101/2022.05.14.491371
PMC
PMC9463103
OpenAlex
W4284881971
Cited by
0
References
1
FWCI
0.00
Type
journal article
Language
en
Open access
Locations
  • Journal of Magnetic Resonance (acceptedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Flight Attendant Medical Research Institute
  • Institute for Clinical and Translational Research, University of Maryland, Baltimore
  • National Institutes of Health (UL1TR003098)
  • National Institute of General Medical Sciences (R01GM104257, T32GM080189, T32GM149382)
  • National Center for Advancing Translational Sciences
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords
chemoenzymatic synthesis, nonribosomal peptide synthetase, NMR pulse sequences, reaction monitoring, in situ NMR, reproducibility, pulse sequence design
MeSH terms
Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy

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