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PreprintCited by 53Bacteriophages and microbial interactionsPlant and Fungal Interactions ResearchEvolution and Genetic Dynamics

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Infection cycle and phylogeny of the Polinton-like virus Phaeocystis globosa virus virophage-14T

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Roitman, Sheila, Rozenberg, Andrey, Lavy, Tali, Brussaard, Corina P. D., Kleifeld, Oded, Béjà, Oded·2022·Nature Microbiology
DOI 10.1101/2022.07.28.501842

Preprint — published as 10.1038/s41564-022-01305-7

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Abstract

Virophages are small dsDNA viruses dependent on a nucleocytoplasmic large-DNA virus infection of a cellular host for replication. Putative virophages infecting algal hosts are classified together with polinton-like viruses, transposable elements widely found in algal genomes, yet the lack of isolated strains raises questions about their existence as independent entities. In this work we isolated and characterized a virophage (PgVV-14T) co-infecting Phaeocystis globosa with the Phaeocystis globosa virus-14T (PgV-14T). PgVV-14T decreases the fitness of its PgV-14T viral host, yet it does not salvage the cellular host population. We found viral-like elements resembling PgVV-14T in Phaeocystis genomes, suggesting that these virophages are capable of integrating to the cellular host genome, bridging the gap between Polinton-like viruses and virophages. This system, with a giant virus, a virophage and endogenous viral elements preying on an algal host, presents an opportunity to gain a better understanding on the evolution of eukaryotes and their viruses.

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Metadata

Authors
Roitman, S., Rozenberg, A., Lavy, T., Brussaard, C. P. D., Kleifeld, O., & Béjà, O.
Affiliations
  • Technion – Israel Institute of Technology (IL)
  • Royal Netherlands Institute for Sea Research (NL)
Corresponding author
  • Roitman, Sheila
  • Béjà, Oded
ORCID
Venue
Nature Microbiology
Publisher
openRxiv
Published
2022-07-28
Volume
8
Issue
2
Pages
332-346
ISSN
2058-5276 (ISSN-L)
DOI
10.1101/2022.07.28.501842
OpenAlex
W4318071672
Cited by
53
References
1
Citations by year
2026: 11 · 2025: 14 · 2024: 14 · 2023: 14
FWCI
8.56
Type
journal article
Language
en
Open access
Locations
  • Nature Microbiology (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • UvA-DARE (University of Amsterdam) (publishedVersion)
  • Flanders Marine Institute (Flanders Marine Institute) (submittedVersion, other-oa)
Funders
  • European Commission (321647)
  • Israel Science Foundation (2167/17, 1623/17, 143/18)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Life Sciences › Immunology and Microbiology › Virology › Plant and Fungal Interactions Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
Keywords
virophages, Polinton-like viruses, Phaeocystis globosa, co-infection, viral integration into host genome, burst size, marine algae, virus isolation
MeSH terms
Virophages, Phylogeny, Viruses, Genome, Viral, Phycodnaviridae, Haptophyta

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