The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Gene amplifications cause high-level resistance against albicidin in Gram-negative bacteria
Open accessPreprint — published as 10.1371/journal.pbio.3002186 · Paper page
Abstract
Antibiotic resistance is a continuously increasing concern for public health care. Understanding resistance mechanisms and their emergence is crucial for the development of new antibiotics and their effective use. Here, we report the discovery of a gene amplification-based mechanism that imparts an up to 1000-fold increase in resistance levels against the antibiotic albicidin. We show that this mechanism protects Salmonella Typhimurium and Escherichia coli by increasing the copy number of the GyrI-like transcription regulator STM3175 (YgiV) which binds albicidin. X-ray crystallography and molecular docking studies reveal a conserved binding motif that can interact with aromatic building blocks of albicidin. Phylogenetic studies suggest that this resistance mechanism is ubiquitous in Gram-negative bacteria and our experiments confirm that STM3175 homologs can convey resistance in pathogens such as Vibrio vulnificus and Pseudomonas aeruginosa .
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Research works printed but not linked: 42
- 10.1371/journal.pmed.1002184 · DOI
- 10.1016/S0140-6736(21)02724-0 · DOI · Paper page
- 10.1146/annurev-genet-102108-134805 · DOI
- 10.1101/cshperspect.a016592 · DOI
- 10.1080/10495390600957092 · DOI
- 10.1099/00221287-131-5-1069 · DOI
- 10.1038/nchembio.1734 · DOI
- 10.1002/chem.201904752 · DOI
- 10.1002/chem.202100523 · DOI
- 10.1039/D1SC04019G · DOI
- 10.1002/chem.202000117 · DOI
- 10.1002/anie.201409964 · DOI
- 10.1021/acs.jnatprod.6b00294 · DOI
- 10.3390/molecules24071390 · DOI
- 10.1002/anie.201409584 · DOI
- 10.1128/AAC.00918-06 · DOI
- 10.1111/j.1365-2672.2006.02899.x · DOI
- 10.1021/jacs.5b04099 · DOI
- 10.1038/s41467-018-05551-4 · DOI
- 10.1099/00221287-136-1-51 · DOI
- 1993, Single amino acid substitutions affecting the substrate specificity of the Escherichia coli K-12 nucleoside-specific Tsx channel, The Journal of biological chemistry, 268, 17495, 10.1016/S0021-9258(19)85361-9 · DOI
- 10.1371/journal.pgen.1008615 · DOI
- 10.1002/prot.22431 · DOI
- 10.1002/prot.10102 · DOI
- 10.1126/science.abj8754 · DOI · Paper page
- 10.1038/nprot.2015.053 · DOI · Paper page
- 10.1038/75213 · DOI
- 10.1093/nar/gkab1061 · DOI · Paper page
- 10.1007/978-1-0716-0270-6_3 · DOI
- 10.1021/acs.bio-chem.6b00651 · DOI
- 10.1101/gr.849004 · DOI
- 10.1038/s41429-019-0228-2 · DOI
- 10.1093/jac/dkg058 · DOI
- 10.1111/j.1365-2958.1995.mmi_18020301.x · DOI
- 10.1038/s41559-020-1132-7 · DOI
- 10.1073/pnas.021448998 · DOI
- 10.1093/oxfordjournals.molbev.a026009 · DOI
- 10.1098/rspb.2012.1108 · DOI
- 10.1534/genetics.109.111963 · DOI
- 10.1038/s41564-018-0342-0 · DOI
- 10.1093/gbe/evu106 · DOI
- 10.1007/s00203-003-0598-4 · DOI
Metadata
- Authors
- Saathoff, M., Kosol, S., Semmler, T., Tedin, K., Dimos, N., Kupke, J., Seidel, M., Ghazisaeedi, F., Wolf, S. A., Kuropka, B., Czyszczoń, W., Ghilarov, D., Grätz, S., Heddle, J. G., Loll, B., Süssmuth, R. D., & Fulde, M.
- Affiliations
- Freie Universität Berlin (DE)
- Technische Universität Berlin (DE)
- Robert Koch Institute (DE)
- Jagiellonian University (PL)
- Corresponding author
- Süssmuth, Roderich D.
- Fulde, Marcus
- ORCID
- Venue
- PLoS Biology
- Publisher
- openRxiv
- Published
- 2022-09-15
- Volume
- 21
- Issue
- 8
- Pages
- e3002186-e3002186
- ISSN
- 1544-9173 (ISSN-L)
- PMC
- PMC10414762
- OpenAlex
- W4385727347
- Cited by
- 25
- References
- 1
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 8 · 2023: 5
- FWCI
- 2.14
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- PLoS Biology (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Homo Politicus (Academy of Humanities and Economics in Lodz) (submittedVersion, cc-by-nc-nd)
- Publication server of the Robert Koch Institute (Robert Kock Institute) (submittedVersion, cc-by)
- Refubium (Universitätsbibliothek der Freien Universität Berlin) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- HZB Repository (Helmholtz-Zentrum Berlin für Materialien und Energie GmbH (HZB)) (submittedVersion, other-oa)
- Universitätsbibliothek der FU Berlin Hochschulschriftenstelle u. Dokumentenserver (cc-by)
- Funders
- European Commission (261572)
- Deutsche Forschungsgemeinschaft (Project B5, 1027/4-1, FU 1027/4-1, SU 239/18-1, SPP2225, priority program SPP2225 (FU 1027/4-1), CRC1449, 431232613)
- Narodowym Centrum Nauki (2020/39/B/NZ1/02898, 2020/39/B/NZ1/02898 (OPUS 20))
- Humboldt-Universität zu Berlin
- Freie Universität Berlin
- H. Wilhelm Schaumann Stiftung
- Instituto de Física de Cantabria
- Horizon 2020 Framework Programme (675858, 101017567, 777536)
- Helmholtz-Zentrum Berlin für Materialien und Energie
- Leibniz-Gemeinschaft
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- albicidin, antibiotic resistance, gene amplification, transcription regulators, Salmonella typhimurium, Escherichia coli, Vibrio vulnificus, Pseudomonas aeruginosa, Gram-negative bacteria
- MeSH terms
- Anti-Bacterial Agents, Gene Amplification, Gram-Negative Bacteria, Gram-Positive Bacteria, Organic Chemicals, Phylogeny, Xanthomonas, Molecular Docking Simulation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
