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PreprintCited by 19Chromosomal and Genetic VariationsPlant Molecular Biology ResearchTelomeres, Telomerase, and Senescence

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Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions

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Kusová, Alžbeta, Steinbachová, Lenka, Přerovská, Tereza, Drábková, Lenka Záveská, Paleček, Jan, Khan, Ahmed, Rigóová, Gabriela, Gadiou, Zuzana, Jourdain, Claire, Stricker, Tino, Schubert, Daniel, Honys, David, Schrumpfová, Petra Procházková·2022·Plant Molecular Biology
DOI 10.1101/2022.11.23.517682

Preprint — published as 10.1007/s11103-023-01348-2

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Abstract

Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as associated with terminal telomeric repeats (telomeres) or short interstitial telomeric repeats in gene promoters ( telo -boxes). They are also known to interact with several protein complexes: telomerase, Polycomb repressive complex 2 (PRC2) E(z) subunits and the PEAT complex (PWOs-EPCRs-ARIDs-TRBs). Here we characterize two novel members of the TRB family (TRB4 and TRB5). Our wide phylogenetic analyses have shown that TRB proteins evolved in the plant kingdom after the transition to a terrestrial habitat in Streptophyta, and consequently TRBs diversified in seed plants. TRB4-5 share common TRB motifs while differing in several others and seem to have an earlier phylogenetic origin than TRB1-3. Their common Myb-like domains bind long arrays of telomeric repeats in vitro, and we have determined the minimal recognition motif of all TRBs as one telo -box. Our data indicate that despite the distinct localization patterns of TRB1-3 and TRB4-5 in situ, all members of TRB family mutually interact and also bind to telomerase/PRC2/PEAT complexes. Additionally, we have detected novel interactions between TRB4-5 and EMF2 and VRN2, which are Su(z)12 subunits of PRC2.

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Metadata

Authors
Kusová, A., Steinbachová, L., Přerovská, T., Drábková, L. Z., Paleček, J., Khan, A., Rigóová, G., Gadiou, Z., Jourdain, C., Stricker, T., Schubert, D., Honys, D., & Schrumpfová, P. P.
Affiliations
  • Masaryk University (CZ)
  • Czech Academy of Sciences (CZ)
  • Freie Universität Berlin (DE)
Corresponding author
Schrumpfová, Petra Procházková
ORCID
Venue
Plant Molecular Biology
Publisher
openRxiv
Published
2022-11-25
Volume
112
Issue
1-2
Pages
61-83
ISSN
0167-4412 (ISSN-L)
DOI
10.1101/2022.11.23.517682
OpenAlex
W4367307695
Cited by
19
References
1
Citations by year
2026: 3 · 2025: 8 · 2024: 6 · 2023: 2
FWCI
3.00
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Plant Molecular Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • Research Square (acceptedVersion, cc-by)
  • Refubium (Universitätsbibliothek der Freien Universität Berlin) (submittedVersion, cc-by)
  • Universitätsbibliothek der FU Berlin Hochschulschriftenstelle u. Dokumentenserver (cc-by)
Funders
  • Ministerstvo Školství, Mládeže a Tělovýchovy (LTAUSA18115, LM2018129, LM2018129Czech-BioImaging, LM2010005, CZ.1.05/3.2.00/08.0144, CZ.1.05)
  • Grantová Agentura České Republiky (GX20-01331X, 1.05/3.2.00/08.0144, GA21-15841S, LM2018129)
  • Akademie Věd České Republiky (LM2010005, LM2018129)
  • Central European Institute of Technology (LM2018129, LM2010005)
  • Masarykova Univerzita (LM2018129)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
Keywords
telomeric repeats, telomerase, polycomb repressive complex 2, protein-protein interaction, phylogenetic evolution, Arabidopsis thaliana, VRN2, subcellular localization

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