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A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors
Open accessPreprint — published as 10.1111/jipb.13645 · Paper page
Abstract
In plants, thousands of nucleus-encoded proteins translated in the cytosol are sorted to chloroplasts and mitochondria by binding to specific receptors of the TOC (translocon at the outer membranes of chloroplasts) and the TOM (translocon at the outer membranes of mitochondria) complexes for import into those organelles. The degradation pathways for these receptors are unclear. Here, we discovered a converged ubiquitin-proteasome pathway for the degradation of Arabidopsis thaliana TOC and TOM tail-anchored receptors. The receptors are ubiquitinated by E3 ligase(s) and pulled from the outer membranes by the AAA + ATPase CDC48, after which a previously characterized cytosolic protein, TTOP, binds to the exposed transmembrane domains (TMDs) at the C termini of the receptors and CDC48, and delivers these complexes to the 26S proteasome.
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Metadata
- Authors
- Yang, M., Chen, S., Lim, S.-L., Yang, L., Zhong, J. Y., Chan, K. C., Zhao, Z., Wong, K.-B., Wang, J., & Lim, B. L.
- Affiliations
- Southern University of Science and Technology (CN)
- Chinese University of Hong Kong (HK)
- University of Hong Kong (HK)
- Corresponding author
- Lim, Boon Leong
- ORCID
- Venue
- Journal of Integrative Plant Biology
- Publisher
- openRxiv
- Published
- 2023-01-08
- Volume
- 66
- Issue
- 5
- Pages
- 1007-1023
- ISSN
- 0577-7496 (ISSN-L)
- OpenAlex
- W4392956512
- Cited by
- 8
- References
- 1
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 1
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Journal of Integrative Plant Biology (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion, cc-by-nc-nd)
- Funders
- Research Grants Council, University Grants Committee (Area of Excellence Scheme AoE/M-403/16)
- Innovation and Technology Commission
- Innovation and Technology Fund
- Science, Technology and Innovation Commission of Shenzhen Municipality (201708183000803)
- State Key Laboratory for Agrobiotechnology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- tail-anchored proteins, Toc complex, TOM complex, chloroplast protein import, mitochondrial protein import, ubiquitin-proteasome system, protein degradation, E3 ubiquitin ligase, transmembrane domain, 26S proteasome, Arabidopsis thaliana, nuclear-encoded chloroplast proteins
- MeSH terms
- Valosin Containing Protein, Arabidopsis, Ubiquitin, Arabidopsis Proteins, Proteasome Endopeptidase Complex, Proteolysis
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