The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 2 localizes to the Golgi
Open accessPreprint — published as 10.1093/plphys/kiad540
Abstract
Mitochondria act as cellular hubs of energy transformation and metabolite conversion in most eukaryotes. Plant mitochondrial electron transport chains are particularly flexible, featuring alternative components, such as ALTERNATIVE NAD(P)H DEHYDROGENASES and ALTERNATIVE OXIDASES (AOXs), that can bypass proton translocation steps. PLANT UNCOUPLING MITOCHONDRIAL PROTEINS (named PUMPs or plant UCPs) have been identified in plants as homologues of mammalian Uncoupling Proteins (UCPs), and their biochemical and physiological roles have been investigated in the context of mitochondrial energy metabolism. To dissect UCP function in Arabidopsis, the two most conserved (UCP1 and UCP2) have been targeted in recent work by combining mutant lines to circumvent potential functional redundancy in vivo . Such approaches rely on the assumption that both proteins reside in the inner mitochondrial membrane as a prerequisite for functional redundancy. Yet, contradicting results have been reported on UCP2 localization in plants. Here we provide evidence that, conversely to UCP1, which is an abundant inner mitochondrial membrane protein, UCP2 localizes to the Golgi rather than to mitochondria. Based on multiple lines of new and prior evidence, we summarize the consensus view that we have reached and provide an example of how open, critical exchange within the research community is able to constructively address ambiguities. Our observations and considerations provide direction to the ongoing discussion about the functions of UCP proteins. They further offer new perspectives for the study of Golgi membrane transport and subcellular targeting principles of membrane proteins. Since 20 to 30 % of genes in plant genomes are predicted to encode transmembrane proteins and the function of most of those proteins has not been experimentally investigated, we highlight the importance of using independent evidence for localization as a prerequisite for understanding physiological function of membrane proteins.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Research works printed but not linked: 75
- 10.1105/tpc.108.062877 · DOI
- 2021, Knockdown of Mitochondrial Uncoupling Proteins 1 and 2 (AtUCP1 and 2) in Arabidopsis thaliana Impacts Vegetative Development and Fertility, Plant Cell Physiol, 62, 1630, 10.1093/pcp/pcab117 · DOI
- 10.1093/pcp/pcr019 · DOI
- 2020, Mitochondrial uncoupling proteindependent signaling in plant bioenergetics and stress response, Mitochondrion, 53, 109, 10.1016/j.mito.2020.05.001 · DOI
- 2022, Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway, Current Biol, 32, 1403, 10.1016/j.cub.2022.01.037 · DOI
- 2022, Metabolism and Signaling of Plant Mitochondria in Adaptation to Environmental Stresses, Int. J. Mol. Sci, 23, 11176, 10.3390/ijms231911176 · DOI
- 2017, Mitochondrial uncoupling protein 1 overexpression increases yield in nicotiana tabacum under drought stress by improving source and sink metabolism, Front. Plant Sci, 8, 1836, 10.3389/fpls.2017.01836 · DOI
- 10.1371/journal.pone.0016163 · DOI
- 10.1016/j.cmet.2014.07.004 · DOI · Paper page
- 10.1007/978-1-62703-580-4_26 · DOI
- 10.1093/jxb/erj070 · DOI
- 2020, Tissue-specific isolation of Arabidopsis/plant mitochondria - IMTACT (isolation of mitochondria tagged in specific cell types), Plant J, 103, 459, 10.1111/tpj.14723 · DOI
- 10.1105/tpc.105.040741 · DOI
- 10.1111/j.1365-313X.2008.03704.x · DOI
- 10.1016/j.plaphy.2013.10.002 · DOI
- 2017, Shuttling of (deoxy-) purine nucleotides between compartments of the diatom Phaeodactylum tricornutum, New Phytol, 213, 193, 10.1111/nph.14126 · DOI
- 10.1007/s11103-005-5514-7 · DOI
- 10.1074/jbc.M301075200 · DOI
- 10.1093/nar/16.22.10881 · DOI
- 10.1016/j.biochi.2016.12.016 · DOI
- 10.1093/nar/gkh340 · DOI · Paper page
- 10.1104/pp.114.243824 · DOI
- 2019, Single organelle function and organization as estimated from Arabidopsis mitochondrial proteomics, Plant J, 101, 1
- 2022, Reductive stress triggers ANAC017-mediated retrograde signaling to safeguard the endoplasmic reticulum by boosting mitochondrial respiratory capacity, Plant Cell, 34, 1375, 10.1093/plcell/koac017 · DOI
- 10.1093/pcp/pce108 · DOI
- 10.1105/tpc.112.101964 · DOI
- Haferkamp, I. , & Schmitz-Esser, S. (2012). The plant mitochondrial carrier family: Functional and evolutionary aspects. Front. Plant Sci. 3. · DOI
- 10.1104/pp.15.00499 · DOI
- 10.1093/nar/gkw1041 · DOI
- Hruz, T. , Laule, O. , Szabo, G. , Wessendorp, F. , Bleuler, S. , Oertle, L. , Widmayer, P. , Gruissem, W. , & Zimmermann, P. (2008). Genevestigator V3 : A Reference Expression Database for the Meta-Analysis of Transcriptomes. Adv. Bioinformatics 2008: 42047. · DOI
- 10.1093/bioinformatics/17.8.754 · DOI · Paper page
- 10.1093/jxb/ern024 · DOI
- 10.1074/jbc.273.52.34882 · DOI
- 10.1074/jbc.271.51.32743 · DOI
- 10.1016/j.cell.2016.06.044 · DOI
- 10.1104/pp.111.182352 · DOI
- 10.1104/pp.19.00732 · DOI
- 2020, Tagging and catching: rapid isolation and efficient labelling of organelles using the covalent Spy-System in planta, Plant Methods, 16, 122, 10.1186/s13007-020-00663-9 · DOI
- 2021, The versatility of plant organic acid metabolism in leaves is underpinned by mitochondrial malate-citrate exchange, Plant Cell, 33, 3700, 10.1093/plcell/koab223 · DOI
- 10.1105/tpc.107.057554 · DOI
- 2022, The Double Knockdown of the Mitochondrial Uncoupling Protein Isoforms Reveals Partial Redundant Roles During Arabidopsis thaliana Vegetative and Reproductive Development, Plant Sci, 322, 111365, 10.1016/j.plantsci.2022.111365 · DOI
- 10.1105/tpc.108.062042 · DOI
- 10.1007/s11738-014-1746-y · DOI
- 10.1016/S0014-5793(98)00634-6 · DOI
- 10.1105/tpc.113.116897 · DOI
- 2022, Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1), ELife, 11, e70701, 10.7554/eLife.70701 · DOI
- 10.1105/tpc.109.066019 · DOI
- 2020, Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria, Biomolecules, 10, 1190, 10.3390/biom10081190 · DOI
- 10.1074/jbc.RA117.000771 · DOI
- 10.1271/bbb.70216 · DOI
- 10.1104/pp.121.4.1127 · DOI
- 10.1111/j.1365-313X.2007.03212.x · DOI
- 10.1104/pp.19.00736 · DOI
- 2012, Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics, Plant Physiol, 60, 1037
- 10.1007/s10863-011-9336-9 · DOI
- 2018, ATTED-II in 2018: A Plant Coexpression Database Based on Investigation of the Statistical Property of the Mutual Rank Index Special Issue - Databases, Plant Cell Physiol, 59, 1
- 10.1111/j.1365-313X.2011.04516.x · DOI
- 10.1042/BJ20070867 · DOI
- 10.1104/pp.111.193151 · DOI
- 10.1105/tpc.111.084574 · DOI
- 10.1093/nar/gku316 · DOI · Paper page
- 2021, ClCd and ClCf act redundantly at the trans-Golgi network/early endosome and prevent acidification of the Golgi stack, J. Cell Sci, 134, jcs258807, 10.1242/jcs.258807 · DOI
- 10.1104/pp.011577 · DOI
- 10.1111/tpj.13448 · DOI
- 10.1093/mp/sst079 · DOI
- 10.1074/jbc.M408920200 · DOI
- 10.1093/MOLBEV/MSZ312 · DOI
- 10.1242/jcs.139907 · DOI
- 10.1105/tpc.20.00241 · DOI
- 10.1073/pnas.0607751103 · DOI
- 10.1146/annurev.arplant.57.032905.105335 · DOI
- 10.1105/tpc.15.00509 · DOI
- Wagner, S. , Nietzel, T. , Aller, I. , Costa, A. , Fricker, M. D. , Meyer, A. J. , & Schwarzländer, M. (2015b). Analysis of plant mitochondrial function using fluorescent protein sensors. In Plant Mitochondria: Methods and Protocols 241–252. · DOI
- 10.1093/oxfordjournals.pcp.a029501 · DOI
- 10.1371/journal.pone.0026477 · DOI
Metadata
- Authors
- Fuchs, P., Feixes-Prats, E., Arruda, P., Feitosa-Araújo, E., Fernie, A. R., Grefen, C., Lichtenauer, S., Linka, N., de Godoy Maia, I., Meyer, A. J., Schilasky, S., Sweetlove, L. J., Wege, S., Weber, A. P., Millar, A. H., Keech, O., Florez Sarasa, I., Barreto, P., & Schwarzländer, M.
- Affiliations
- University of Bonn (DE)
- Universitat Autònoma de Barcelona (ES)
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Max Planck Society (DE)
- Ruhr University Bochum (DE)
- University of Münster (DE)
- Cluster of Excellence on Plant Sciences (DE)
- University of Oxford (GB)
- The University of Western Australia (AU)
- Umeå Plant Science Centre (SE)
- Corresponding author
- Barreto, Pedro
- Schwarzländer, Markus
- ORCID
- Fuchs, Philippe
- Feixes-Prats, Elisenda
- Arruda, Paulo
- Feitosa-Araújo, Elias
- Fernie, Alisdair R.
- Grefen, Christopher
- Lichtenauer, Sophie
- Linka, Nicole
- Meyer, Andreas J.
- Schilasky, Sören
- Sweetlove, Lee J.
- Wege, Stefanie
- Weber, Andreas P.
- Millar, A. Harvey
- Keech, Olivier
- Florez Sarasa, Igor
- Barreto, Pedro
- Schwarzländer, Markus
- Venue
- PLANT PHYSIOLOGY
- Publisher
- openRxiv
- Published
- 2023-05-08
- Volume
- 194
- Issue
- 2
- Pages
- 623-628
- ISSN
- 0032-0889 (ISSN-L)
- OpenAlex
- W4387520759
- Cited by
- 3
- References
- 1
- Citations by year
- 2025: 1 · 2024: 2
- FWCI
- 0.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- PLANT PHYSIOLOGY (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- UWA Profiles and Research Repository (UWA) (submittedVersion, other-oa)
- HAL (submittedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- IRTA (Institut de Recerca i Tecnologia Agroalimentaries) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SCHW 1719/9-1, MCIN /AEI/10.13039/501100011033, GRK2064)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (MCIN/AEI/10.13039/501100011033, AEI/10.13039/501100011033)
- Westfälische Wilhelms-Universität Münster (RI_00497)
- Agencia Estatal de Investigación (AEI//10.13039/501100011033/, 501100011033, 10.13039/501100011033, MCIN/AEI/10.13039/501 100 011 033, 13039, .13039/501100011033, 10.13039, AEI/10)
- European Social Fund (MCIN/AEI/10.13039/ 501100011033, 501100011033, AEI/10.13039/501100011033)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- UCP2, Golgi apparatus, protein localization, UCP1, mitochondrial carrier protein, plant mitochondria
- MeSH terms
- Golgi Apparatus, Humans, Membrane Proteins, Mitochondrial Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
