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PreprintCited by 30Plant Molecular Biology ResearchGenomics and Chromatin DynamicsEpigenetics and DNA Methylation

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

A CPF-like phosphatase module links transcription termination to chromatin silencing

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Mateo Bonmati, Eduardo, Montez, Miguel, Maple, Robert, Fiedler, Marc, Fang, Xiaofeng, Saalbach, Gerhard, Passmore, Lori A, Dean, Caroline A·2023·Molecular Cell
DOI 10.1101/2023.07.06.547976PMC PMC7616277

Preprint — published as 10.1016/j.molcel.2024.05.016 · Paper page

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Abstract

The interconnections between co-transcriptional regulation, chromatin environment and transcriptional output remain poorly understood. Here, we investigate the mechanism underlying RNA 3 processing-mediated Polycomb silencing of Arabidopsis FLOWERING LOCUS C (FLC). We show a requirement for APRF1, a homologue of yeast Swd2 and human WDR82, known to regulate RNA Pol II during transcription termination. APRF1 interacts with TOPP4 (yeast Glc7/human PP1) and LD, the latter showing structural features found in Ref2/PNUTS; all components of the yeast and human phosphatase module of the CPF 3 end processing machinery. LD has been shown to co-associate in vivo with the histone H3 K4 demethylase FLD. We show APRF1 and LD couple CPF-mediated cleavage and polyadenylation with removal of H3K4 monomethylation in the body of FLC, and this influences subsequent transcription. This work shows how transcription termination can change the local chromatin environment to modulate transcription of Arabidopsis FLC and affect flowering time.

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Metadata

Authors
Mateo Bonmati, E., Montez, M., Maple, R., Fiedler, M., Fang, X., Saalbach, G., Passmore, L. A., & Dean, C. A.
Affiliations
  • John Innes Centre (GB)
  • MRC Laboratory of Molecular Biology (GB)
Corresponding author
Dean, Caroline A
ORCID
Venue
Molecular Cell
Publisher
openRxiv
Published
2023-07-06
Volume
84
Issue
12
Pages
2272-2286.e7
ISSN
1097-2765 (ISSN-L)
DOI
10.1101/2023.07.06.547976
PMC
PMC7616277
OpenAlex
W4399430120
Cited by
30
References
1
Citations by year
2026: 11 · 2025: 10 · 2024: 9
FWCI
7.32
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Molecular Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion, cc-by)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
Funders
  • Wellcome Trust (210654, 225217/Z/22/Z, 210654/Z/18/Z)
  • Friends Research Institute
  • Royal Society (RP\R1\180002, RP\u2216R1\u2216180002)
  • European Commission
  • Ministerio de Ciencia e Innovación
  • Medical Research Council (225217/Z/22/Z, MC_U105192715)
  • Biotechnology and Biological Sciences Research Council (BB/J0045881, BBP0135111, BB/J004588, BB/P013511, BBS/E/JI/230002B)
  • European Research Council
  • Agencia Estatal de Investigación
  • H2020 European Research Council (833254)
  • NextGenerationEU
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
FLOWERING LOCUS C, Polycomb silencing, transcription termination, RNA 3' end processing, chromatin silencing, polyadenylation, RNA polymerase II, FLOWERING LOCUS D, Arabidopsis thaliana, co-transcriptional regulation
MeSH terms
Chromatin, Histone Deacetylases, Histones, Phosphoprotein Phosphatases, RNA Polymerase II, Arabidopsis, Gene Expression Regulation, Plant, Gene Silencing, MADS Domain Proteins, Arabidopsis Proteins, mRNA Cleavage and Polyadenylation Factors, Transcription Termination, Genetic

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