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PreprintCited by 4Protein Structure and DynamicsBacterial Genetics and BiotechnologyEnzyme Structure and Function

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The S-component fold: A link between bacterial transporters and receptors

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Partipilo, Michele, Slotboom, Dirk Jan·2023·Communications Biology
DOI 10.1101/2023.12.20.572563PMC PMC11109136

Preprint — published as 10.1038/s42003-024-06295-2 · Paper page

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Abstract

The processes of nutrient uptake and signal sensing are crucial for microbial survival and adaptation. Membrane-embedded proteins involved in these functions (transporters and receptors) are commonly regarded as unrelated in terms of sequence, structure, mechanism of action and evolutionary history. Here, we analyze the protein structural universe using recently developed artificial intelligence-based structure prediction tools, and find an unexpected link between prominent groups of microbial transporters and receptors. The so-called S-components of energy-coupling factor (ECF) transporters, and the membrane domains of sensor histidine kinases of the 5TMR cluster share a structural fold. The discovery of their relatedness manifests a widespread case of prokaryotic “transceptors” (related proteins with transport or receptor function), showcases how artificial intelligence-based structure predictions reveal unchartered evolutionary connections between proteins, and provides new avenues for engineering transport and signaling functions in bacteria.

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Metadata

Authors
Partipilo, M., & Slotboom, D. J.
Affiliations
University of Groningen (NL)
Corresponding author
Slotboom, Dirk Jan
ORCID
Venue
Communications Biology
Publisher
openRxiv
Published
2023-12-20
Volume
7
Issue
1
Pages
610-610
ISSN
2399-3642 (ISSN-L)
DOI
10.1101/2023.12.20.572563
PMC
PMC11109136
OpenAlex
W4398173255
Cited by
4
References
1
Citations by year
2025: 2 · 2024: 2
FWCI
0.54
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Communications Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (714.018.003.)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
Keywords
sensor histidine kinase, transceptors, bacterial membrane transporters, bacterial receptors, protein structural evolution, nutrient uptake, signal sensing
MeSH terms
Histidine Kinase, Artificial Intelligence, Bacteria, Bacterial Proteins, Models, Molecular, Signal Transduction, Protein Folding, Membrane Transport Proteins

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