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Architecture of the spinach plastid-encoded RNA polymerase
Open accessPreprint — published as 10.1038/s41467-024-54266-2 · Paper page
Abstract
The plastid-encoded RNA polymerase (PEP) serves as the principal transcription machinery within chloroplasts and is responsible for transcribing over 80% of primary plastid transcripts. Plant PEP is composed of a prokaryotic-like multisubunit core enzyme known as the PEP core, supplemented by newly evolved PEP-associated proteins (PAPs). In this study, we present the cryo-electron microscopy (cryo-EM) structure of a PEP complex derived from spinach (Spinacia oleracea). Our structural analysis unveils the presence of 14 PAPs closely associated with the PEP core complex consisting of 5 subunits, forming an irregular-shaped supercomplex. We provide a detailed depiction of the PEP core subunits and the 14 PAPs, elucidating their precise spatial arrangement and interactions. This research offers a crucial structural basis for future investigations into the functions and regulatory mechanisms governing plastid transcription.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
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Metadata
- Authors
- Wang, T., Wang, G., Fang, Y., Zhang, Y., Peng, W., Zhou, Y., Zhang, A., Yu, L.-J., & Lu, C.
- Affiliations
- Shandong Agricultural University (CN)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Yu, Long-Jiang
- Lu, Congming
- Venue
- Nature Communications
- Publisher
- openRxiv
- Published
- 2024-02-17
- Volume
- 15
- Issue
- 1
- Pages
- 9838-9838
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC11561172
- OpenAlex
- W4404311285
- Cited by
- 17
- References
- 1
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 1
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Nature Communications (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Natural Science Foundation of China (32000184, 32000444)
- Natural Science Foundation of Shandong Province (ZR2019ZD48)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- plastid-encoded RNA polymerase, chloroplast transcription, PAPS, spinach, oxidative damage protection, transcriptional regulation, chloroplast gene expression
- MeSH terms
- Chloroplasts, Models, Molecular, Plant Proteins, DNA-Directed RNA Polymerases, Transcription, Genetic, Plastids, Spinacia oleracea, Cryoelectron Microscopy
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