CoChat
Sign Up
PreprintCited by 1Genomics and Chromatin DynamicsMicrotubule and mitosis dynamicsNuclear Structure and Function

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Cell cycle and Age-Related Modulations of Mouse Chromosome Stiffness

Open access
Liu, Ning, Qiang, Wenan, Jordan, Philip, Marko, John F., Qiao, Huanyu·2024·eLife
DOI 10.1101/2024.03.06.583771PMC PMC10979861

Preprint — published as 10.7554/elife.97403.3 · Paper page

Open this paper in CoChat — free account

Abstract

Chromosome structure is complex, and many aspects of chromosome organization are still not understood. Measuring the stiffness of chromosomes offers valuable insight into their structural properties. In this study, we analyzed the stiffness of chromosomes from metaphase I (MI) and metaphase II (MII) oocytes. Our results revealed a ten-fold increase in stiffness (Young’s modulus) of MI chromosomes compared to somatic chromosomes. Furthermore, the stiffness of MII chromosomes was found to be lower than that of MI chromosomes. We examined the role of meiosis-specific cohesin complexes in regulating chromosome stiffness. Surprisingly, the stiffness of chromosomes from three meiosis-specific cohesin mutants did not significantly differ from that of wild-type chromosomes, indicating that these cohesins may not be primary determinants of chromosome stiffness. Additionally, our findings revealed an age-related increase of chromosome stiffness for MI oocytes. Since aging is associated with elevated levels of DNA damage, we investigated the impact of etoposide-induced DNA damage on chromosome stiffness and found that it led to a reduction in stiffness in MI oocytes. Overall, our study underscores the dynamic and cyclical nature of chromosome stiffness, modulated by both the cell cycle, and by age-related factors.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2024.03.06.583771

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 73

  1. 10.1016/B978-0-12-410523-2.00006-7 · DOI
  2. 10.3390/biology6010012 · DOI
  3. 10.2147/tacg.s8884 · DOI
  4. 10.1002/bies.201500020 · DOI
  5. B. Alberts , A. Johnson , J. Lewis , M. Raff , K. Roberts , P. Walter , Chromosomal DNA and Its Packaging in the Chromatin Fiber, (2002). https://www.ncbi.nlm.nih.gov/books/NBK26834/ (accessed February 20, 2023).
  6. 10.1083/jcb.111.6.2839 · DOI
  7. 10.1016/j.semcdb.2021.03.014 · DOI
  8. 10.1016/j.tcb.2019.05.007 · DOI
  9. 10.1101/sqb.1978.042.01.036 · DOI
  10. 10.1016/S1534-5807(03)00092-3 · DOI
  11. 10.1016/0092-8674(77)90280-X · DOI
  12. 10.1083/jcb.96.1.84 · DOI
  13. 10.1091/mbc.E18-09-0592 · DOI
  14. 10.1073/pnas.232442599 · DOI
  15. 10.1016/j.ceb.2016.01.013 · DOI
  16. 10.1146/annurev-genet-061323-044915 · DOI
  17. 10.1091/mbc.8.11.2217 · DOI
  18. 10.1146/annurev.cellbio.12.1.305 · DOI
  19. 10.1038/s42003-020-0953-x · DOI
  20. 10.1016/S0955-0674(96)80015-9 · DOI
  21. 10.1101/gad.345488.120 · DOI
  22. 10.1101/gad.1724308 · DOI
  23. 10.1038/embor.2011.2 · DOI
  24. 10.1038/s41580-019-0132-4 · DOI
  25. 10.1262/jrd.2016-127 · DOI
  26. 10.1016/j.cub.2016.05.006 · DOI
  27. 10.1097/MCO.0b013e32834121b1 · DOI
  28. 10.1016/j.mad.2011.04.008 · DOI
  29. 10.1016/0167-4943(83)90012-2 · DOI
  30. 10.7554/eLife.73524 · DOI
  31. 10.1016/j.cub.2010.08.023 · DOI
  32. 10.1016/j.cub.2017.02.025 · DOI
  33. 10.1007/s10815-015-0453-y · DOI
  34. 10.1007/s10577-018-9584-1 · DOI
  35. 10.7554/eLife.63972 · DOI
  36. 10.1016/j.cub.2008.06.013 · DOI
  37. 10.1101/gad.329705 · DOI
  38. 10.1371/journal.pbio.0030086 · DOI
  39. 10.1242/jcs.00495 · DOI
  40. 10.1534/g3.116.029462 · DOI
  41. 10.1016/j.devcel.2005.03.018 · DOI
  42. 10.1371/journal.pgen.1004413 · DOI
  43. 10.1038/emboj.2011.222 · DOI
  44. 10.4103/2096-2924.327880 · DOI
  45. 10.1371/journal.pone.0096710 · DOI
  46. 10.1038/ncomms6945 · DOI
  47. 10.1093/humrep/dez308 · DOI
  48. 10.1002/bies.20264 · DOI
  49. 10.1200/JCO.1994.12.1.60 · DOI
  50. 10.1093/oxfordjournals.annonc.a059180 · DOI
  51. 10.1093/nar/gkaa1202 · DOI
  52. 10.1371/journal.pgen.1009744 · DOI
  53. 10.7554/eLife.20010 · DOI
  54. 10.1073/pnas.0402724101 · DOI
  55. 10.1091/mbc.E05-04-0321 · DOI
  56. 10.1091/mbc.E11-05-0423 · DOI
  57. 10.1371/journal.pgen.1003538 · DOI
  58. 10.18632/ONCOTARGET.28195 · DOI · Paper page
  59. 10.18632/aging.100372 · DOI
  60. 10.1007/978-3-319-55530-0_123 · DOI
  61. 10.1038/s41392-022-01211-8 · DOI
  62. 10.1530/REP-17-0153 · DOI
  63. 10.1016/j.cub.2009.12.044 · DOI
  64. 10.1371/journal.pgen.1005944 · DOI
  65. 10.1007/s10577-010-9179-y · DOI
  66. 10.1038/aja.2011.66 · DOI
  67. 10.1038/s41389-018-0072-4 · DOI
  68. 10.1073/pnas.1018793108 · DOI
  69. 10.1016/j.jgg.2020.04.003 · DOI
  70. 10.1073/pnas.1204686109 · DOI
  71. 10.1016/bs.mcb.2018.03.002 · DOI
  72. 10.1101/gad.255554.114 · DOI
  73. 10.1016/S0960-9822(00)00799-5 · DOI

Metadata

Authors
Liu, N., Qiang, W., Jordan, P., Marko, J. F., & Qiao, H.
Affiliations
  • University of Illinois Urbana-Champaign (US)
  • Northwestern University (US)
  • Johns Hopkins University (US)
Corresponding author
  • Marko, John F.
  • Qiao, Huanyu
ORCID
Venue
eLife
Publisher
openRxiv
Published
2024-03-11
Volume
13
ISSN
2050-084X (ISSN-L)
DOI
10.1101/2024.03.06.583771
PMC
PMC10979861
OpenAlex
W4409410025
Cited by
1
References
1
Citations by year
2024: 1
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by)
Funders
  • National Institutes of Health (CA193419)
  • National Human Genome Research Institute (UM1HG011536)
  • National Cancer Institute (U54CA193419, U54CA203000)
  • National Institute of General Medical Sciences (R01GM135549, R01GM105847, R01GM117155)
  • National Institute of Environmental Health Sciences (T32ES007326)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R00HD082375, K99HD082375)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
chromosome mechanics, meiosis, metaphase I oocytes, metaphase II oocytes, cohesin, aging, DNA damage, etoposide, Young's modulus, cell cycle

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review