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Jasmonic acid signalling is targeted by a smut fungal Tin2-fold effector
Open accessPreprint — published as 10.1093/jxb/eraf381
Abstract
In plants, jasmonate signaling is a hub integrating environmental cues with growth and development. Due to its role in balancing defense responses against pathogens, it is a target of effector proteins from various pathogens. Here, we characterized the fungal effector protein Tue1 from the Brassicaceae smut fungus Thecaphora thlaspeos . T. thlaspeos naturally infects Arabis hirsuta but can also colonize the non-host Arabidopsis thaliana . In planta, the fungal protein Tue1 hijacks the plant importin-α dependent nuclear transporter to reach the plant nucleus. It interacts with jasmonate ZIM domain 10 (JAZ10) proteins of both A. thaliana and Ar. hirsuta . Structure-guided analysis of Tue1 suggests that it binds the Jas motif of JAZ10 indicating a role in stabilization or binding competition with proteins like MYC3 and COI1. A subset of jasmonate-responsive genes is differentially regulated during T. thlaspeos infection, proposing a link of the Tue1 function to infection. Tue1 share structural similarity to the Tin2-fold family recently described in the corn smut Ustilago maydis . Our study therefore suggests that this structural effector family is expanded across fungal pathogens, although future studies have to reveal whether targeting JAZ-repressors is a conserved mechanism or specifically acquired as an adaptation to its perennial host.
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Metadata
- Authors
- Gul, S., Mendoza-Rojas, G., Heßler, N., Galle, S., Smits, S. H. J., Altegoer, F., & Göhre, V.
- Affiliations
- Cluster of Excellence on Plant Sciences (DE)
- Heinrich Heine University Düsseldorf (DE)
- Darmstadt University of Applied Sciences (DE)
- Corresponding author
- Altegoer, Florian
- Göhre, Vera
- ORCID
- Venue
- Journal of Experimental Botany
- Publisher
- openRxiv
- Published
- 2024-07-08
- Volume
- 76
- Issue
- 22
- Pages
- 7051-7066
- ISSN
- 0022-0957 (ISSN-L)
- OpenAlex
- W4400474916
- Cited by
- 1
- References
- 1
- Citations by year
- 2024: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Journal of Experimental Botany (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- DESY Publication Database (PUBDB) (Deutsches Elektronen-Synchrotron) (submittedVersion, other-oa)
- Funders
- Schlumberger Foundation
- Deutsche Forschungsgemeinschaft (INST 208/740-1, 458090666, INST 208/868-1, 391465903, EXC-2048, SFB1535, EXC-2048/1, GRK 2466, INST 208/740‐1 FUGG, 417919780, 2048/1, INST 208/868-1 FUGG)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Fungal Diseases
- Keywords
- jasmonic acid signaling, fungal effector proteins, JAZ repressors, Arabis hirsuta, Arabidopsis thaliana, MYC3, COI1, plant-pathogen interaction, gene expression regulation
- MeSH terms
- Basidiomycota, Cyclopentanes, Fungal Proteins, Plant Diseases, Signal Transduction, Arabidopsis, Arabidopsis Proteins, Oxylipins
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