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Bone mechano-response is driven by locomotion transitions during vertebrate evolution
Open accessPreprint — published as 10.1038/s42003-025-09292-1 · Paper page
Abstract
The skeleton supports the muscles in keeping the body upright against gravity while enduring thousands of daily loads. In this study, we investigated non-collagenous bone matrix proteins using osteoblast cell cultures and phylogenetic analyses to identify the molecular mechanisms involved in mechanical loading. The results indicate that the bone mechano-response is an evolutionary-driven process and that several non-collagenous proteins may significantly regulate the bone’s response to mechanical stress. According to our results, two significant evolutionary transitions in vertebrate locomotion shaped the roles of non-collagenous proteins in humans: the water-to-land transition, which increased mechanical stress on the limbs, and the evolution to bipedalism in humans, which altered the distribution of stress on the lower and upper limbs. Fetuin A, positively selected in both evolutionary transitions, showed the most significant expression change during mechanical stimulation.
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Metadata
- Authors
- Shimochi, S., Brunet, C., Fontcuberta-Rigo, M., Hrovat, K., Puigbò, P., & Nakamura, M.
- Affiliations
- University of Turku (FI)
- Universitat Autònoma de Barcelona (ES)
- Corresponding author
- Puigbò, Pere
- Nakamura, Miho
- Venue
- Communications Biology
- Publisher
- openRxiv
- Published
- 2024-08-30
- Volume
- 9
- Issue
- 1
- Pages
- 31-31
- ISSN
- 2399-3642 (ISSN-L)
- PMC
- PMC12780083
- OpenAlex
- W4416821153
- Cited by
- 1
- References
- 1
- Citations by year
- 2026: 1
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- www.biorxiv.org/about/FAQ#license (vor)
- Locations
- Communications Biology (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- dCOBISS.SI Digital Repository (submittedVersion, cc-by-nc-nd)
- Dipòsit Digital de Documents de la UAB (Universitat Autònoma de Barcelona) (submittedVersion, cc-by-nc-nd)
- DiRROS repository (University of Maribor) (submittedVersion, cc-by-nc-nd)
- UTUPub (University of Turku) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- China Scholarship Council
- Sigrid Juséliuksen Säätiö (230131)
- Murata Science Foundation (M20-072)
- Japan Society for the Promotion of Science (23K08670)
- Erasmus+
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Bone and Dental Protein Studies
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- vertebrate evolution, locomotion evolution, water-to-land transition, bipedalism, fetuin-A, positive selection, osteoblasts, mechanical loading, osteogenic differentiation
- MeSH terms
- Animals, Biomechanical Phenomena, Bone and Bones, Biological Evolution, Humans, Locomotion, Osteoblasts, Phylogeny, Stress, Mechanical, Vertebrates
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