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PreprintCited by 1Bacterial Identification and Susceptibility TestingBacterial Infections and VaccinesNeonatal and Maternal Infections

Next-Generation Sequencing Could be a Promising Diagnostic Approach for Pathogen Detection: Pathogenic Analysis of Pediatric Bacterial Meningitis by Next-Generation Sequencing Technology Directly from Cerebrospinal Fluid Specimens

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Guo, Ling-yun, Li, Yong-jun, Liu, Lin-lin, Wu, Hong-long, Zhou, Jia-li, Zhang, Ye, Feng, Wen-ya, Zhu, Liang +7 more·2018·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/340273
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Abstract

Background Bacterial meningitis remains one of the major challenges in infectious diseases, leading to sequel in many cases. A prompt diagnosis of the causative microorganism is critical to significantly improve outcome of bacterial meningitis. Although various targeted tests for cerebrospinal fluid (CSF) samples are available, it is a big problem for the identification of etiology of bacterial meningitis. Methods Here we describe the use of unbiased sequence analyses by next-generation sequencing (NGS) technology for the identification of infectious microorganisms from CSF samples of pediatric bacterial meningitis patients in the Department of Infectious Diseases from Beijing Children’s Hospital. Results In total, we had 99 bacterial meningitis patients in our study, 55 (55.6%) of these were etiologically confirmed by clinical microbiology methods. Combined with NGS, 68 cases (68.7%) were etiologically confirmed. The main pathogens identified in this study were Streptococcus pneumoniae (n=29), group B streptococcus (n=15), Staphylococcus aureus (n=7), Escherichia coli (n=7). In addition, two cases with cytomegalovirus infection and one with Taenia saginata asiatica were confirmed by NGS. Conclusions NGS could be a promising alternative diagnostic approach for critically ill patients suffering from bacterial meningitis in pediatric population. Summary We conducted the study for the identification of microorganisms by next-generation sequencing directly from CSF samples of ped…

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  • Physicians' fear of cerebral herniation following LP is an important factor leading to unnecessary neuroimaging.

    “Physicians' fear of cerebral herniation following LP, leading to unnecessary neuroimaging, is also an important factor.63”

    From Community-acquired acute bacterial meningitis in adults: a clinical update (2019) · cites this paper as [63]

  • Expanded use of whole genome sequencing has the greatest potential to transform meningitis diagnostics, particularly prediction of genotypic drug resistance.

    “it is the expanded use of whole genome sequencing in clinical care as it becomes increasingly rapid, accessible, affordable and directly applicable to clinical specimens without the need for culture that has the greatest potential to transform meningitis diagnostics, particularly prediction of genotypic drug resistance 63”

    From Community-acquired acute bacterial meningitis in adults: a clinical update (2019) · cites this paper as [63]

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Authors
Guo, L.-Y., Li, Y.-J., Liu, L.-L., Wu, H.-L., Zhou, J.-L., Zhang, Y., Feng, W.-Y., Zhu, L., Hu, B., Hu, H.-L., Chen, T.-M., Guo, X., Chen, H.-Y., Yang, Y.-H., & Liu, G.
Affiliations
Capital Medical University (CN)
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2018-06-06
DOI
10.1101/340273
OpenAlex
W2807582335
Cited by
0
References
1
Type
posted content
Language
en
Open access
Locations
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Neonatal and Maternal Infections
Keywords
bacterial meningitis, pediatric meningitis, cerebrospinal fluid, pathogen detection, metagenomic sequencing, etiology identification, Streptococcus pneumoniae, Streptococcus agalactiae, Staphylococcus aureus, Escherichia coli, cytomegalovirus, Taenia asiatica, molecular diagnosis

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