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PreprintCited by 136Soil Carbon and Nitrogen DynamicsBioenergy crop production and managementSoil erosion and sediment transport

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Microbial extracellular polysaccharide production and aggregate stability controlled by Switchgrass ( Panicum virgatum ) root biomass and soil water potential

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Sher, Yonatan, Baker, Nameer R., Herman, Don, Fossum, Christina, Hale, Lauren, Zhang, Xing-Xu, Nuccio, Erin, Saha, Malay, Zhou, Jizhong, Pett-Ridge, Jennifer, Firestone, Mary·2019·Soil Biology and Biochemistry
DOI 10.1101/724195

Preprint — published as 10.1016/j.soilbio.2020.107742

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Abstract

Deep-rooting perennial grasses are promising feedstock for biofuel production, especially in marginal soils lacking organic material, nutrients, and/or that experience significant water stress. Perennial grass roots can alter surrounding soil conditions and influence microbial activities, particularly the production of extracellular polymeric substances composed primarily of extracellular polysaccharides (EPS). These polymers can alleviate cellular moisture and nutrient stress, and enhance soil characteristics through improved water retention and aggregate stability, the latter of which may in turn enhance carbon persistence. In this study we used a 13 CO 2 tracer greenhouse experiment to examine the effect of switchgrass cultivation on the production and origin of EPS in a marginal soil with five fertilization/water treatments (control, +N, +NP, +P, low water). Soils with both added nitrogen and phosphorus had the highest root biomass, EPS and percentage of water-stable soil aggregates. Multiple linear regression analyses revealed root biomass was the most important determinant for soil EPS production, potentially by controlling carbon supply and diurnal changes in soil water potential. Path analysis highlighted the role of soil water potential were and EPS on with water-stable soil aggregates, indicating that EPS concentration and soil aggregation have similar drivers in this soil. High mannose content confirmed the microbial origin of EPS. 13 CO 2 labeling indicated that 0.18% of newly fixed plant carbon was incorporated into EPS. Analysis of field samples suggests that EPS is significantly enhanced under long-term switchgrass cultivation. Our results demonstrate that switchgrass cultivation can promote microbial production of EPS, providing a mechanism to enhance sustainability of marginal soils.

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Metadata

Authors
Sher, Y., Baker, N. R., Herman, D., Fossum, C., Hale, L., Zhang, X.-X., Nuccio, E., Saha, M., Zhou, J., Pett-Ridge, J., & Firestone, M.
Affiliations
  • Migal - Galilee Technology Center (IL)
  • University of California, Berkeley (US)
  • Agricultural Research Service (US)
  • Lawrence Livermore National Laboratory (US)
  • Noble Research Institute (US)
  • Lawrence Berkeley National Laboratory (US)
Corresponding author
Baker, Nameer R.
ORCID
Venue
Soil Biology and Biochemistry
Publisher
openRxiv
Published
2019-08-05
Volume
143
Pages
107742-107742
ISSN
0038-0717 (ISSN-L)
DOI
10.1101/724195
OpenAlex
W3004872554
Cited by
136
References
2
Citations by year
2026: 26 · 2025: 25 · 2024: 22 · 2023: 16 · 2022: 23 · 2021: 17 · 2020: 6
FWCI
9.16
Type
journal article
Language
en
Open access
Locations
  • Soil Biology and Biochemistry (acceptedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion, cc-by)
  • Lanzhou University Institutional Repository (submittedVersion, cc-by-nc-sa)
  • Lanzhou University Institutional Repository (submittedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • U.S. Department of Energy (DEAC5207NA27344, DESC0014079, AC52‐07NA27344, SCW1555, Contract DE-AC52-07NA27344)
  • U.S. Department of Agriculture (2016-67012-24717)
  • University of California Berkeley
  • Oklahoma State University
  • University of Oklahoma
  • Noble Research Institute
  • National Institute of Food and Agriculture (2016-67012-24717)
  • Office of Science (DE-AC52-07NA27344, DESC0014079)
  • Lawrence Livermore National Laboratory (DE‐AC5207NA27344, 07NA27344, Contract DE-AC52-07NA27344, SCW1555, DE-AC52, AC52-07NA27344)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Bioenergy crop production and management
  • Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil erosion and sediment transport
Keywords
exopolysaccharides, extracellular polymeric substances, switchgrass, root biomass, soil aggregate stability, soil water potential, marginal soils, biofuel feedstock, nitrogen fertilization, phosphorus fertilization, microbial carbon use, mannose, soil carbon persistence, perennial grasses

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