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PreprintCited by 11Bacterial Genetics and BiotechnologyBacteriophages and microbial interactionsMicrobial Community Ecology and Physiology

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Dinoroseobacter shibae outer membrane vesicles are enriched for the chromosome dimer resolution site dif

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Wang, Hui, Beier, Nicole, Bödeker, Christian, Sztajer, Helena, Henke, Petra, Neumann-Schaal, Meina, Mansky, Johannes, Rohde, Manfred, Overmann, Jörg, Kucklick, Martin, Engelmann, Susanne, Tomasch, Jürgen, Wagner-Döbler, Irene·2019·mSystems
DOI 10.1101/764696PMC PMC7901474

Preprint — published as 10.1128/msystems.00693-20 · Paper page

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Abstract

Outer membrane vesicles (OMVs) of Gram-negative bacteria have key roles in pathogenesis. However, little is known about their biogenesis and cargo in marine bacteria. In Dinoroseobacter shibae, a marine member of the Rhodobacteraceae , OMVs were produced throughout exponential growth, and DNA could be detected by fluorescence microscopy inside appr. 65% of vesicles. Single cell analysis using time-lapse microscopy showed that individual cells secreted multiple OMVs, preferentially at the septum during cell division. OMVs were enriched for saturated fatty acids, thus their secretion likely increases the fluidity of the membrane of the releasing cell locally. DNA was isolated from the vesicle lumen and sequenced; it was up to 40fold enriched for the region around the terminus of replication ( ter ). Within this region, the peak of coverage of vesicle DNA was located at dif, a conserved 28 bp palindromic sequence required for binding of the site specific tyrosine recombinases XerCD which are activated by the divisome protein FtsK immediately prior to septum formation. Some of the most abundant proteins of the vesicle proteome were predicted to be required for direct interaction with peptidoglycan during cell division. Single cell analysis, electron microscopy, proteome and DNA cargo show that constitutive OMV secretion in D. shibae occurs mainly prior to septum formation. The footprint of the FtsK/XerCD molecular machinery which resolves chromosome dimers suggests a novel highly conserved route for incorporation of DNA into OMVs. Clearing the division site from small DNA fragments might be an important function of this type of vesicles.

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Metadata

Authors
Wang, H., Beier, N., Bödeker, C., Sztajer, H., Henke, P., Neumann-Schaal, M., Mansky, J., Rohde, M., Overmann, J., Kucklick, M., Engelmann, S., Tomasch, J., & Wagner-Döbler, I.
Affiliations
  • Technische Universität Braunschweig (DE)
  • Helmholtz Centre for Infection Research (DE)
  • Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DE)
Corresponding author
Tomasch, Jürgen
ORCID
Venue
mSystems
Publisher
openRxiv
Published
2019-09-20
Volume
6
Issue
1
ISSN
2379-5077 (ISSN-L)
DOI
10.1101/764696
PMC
PMC7901474
OpenAlex
W3121018912
Cited by
11
References
1
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 1
FWCI
0.99
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • mSystems (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • HZI (Helmholtz Centre for Infection Research) (submittedVersion, cc-by)
Funders
Deutsche Forschungsgemeinschaft (TRR51, Transregio TRR 51, Transregio-SFB 51)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
Keywords
Dinoroseobacter shibae, outer membrane vesicles, chromosome dimer resolution, divisome, DNA excision, dinoflagellate symbionts

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