The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro
Open accessAbstract
To identify novel inhibitors of transcriptional activation by the HIV Tat protein, we used a combination of in vitro and in vivo Tat-dependent transcription assays to screen >100,000 compounds. All compounds identified blocked Tat-dependent stimulation of transcriptional elongation. Analysis of a panel of structurally diverse inhibitors indicated that their target is the human homolog of Drosophila positive transcription elongation factor b (P-TEFb). Loss of Tat transactivation in extracts depleted of the kinase subunit of human P-TEFb, PITALRE, was reversed by addition of partially purified human P-TEFb. Transfection experiments with wild-type or kinase knockout PITALRE demonstrated that P-TEFb is required for Tat function. Our results suggest that P-TEFb represents an attractive target for the development of novel HIV therapeutics.
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Citations
Cites
Control of RNA Polymerase II Elongation Potential by a Novel Carboxyl-terminal Domain Kinase
Marshall, N. F., Peng, J., Xie, Z., & Price, D. H. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.43.27176 · Cited by 662
Self-citationFeinberg, M. B., Baltimore, D., & Frankel, A. D. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.9.4045 · Cited by 365
Cited by
Wada, T., Takagi, T., Yamaguchi, Y., Ferdous, A., Imai, T., Hirose, S., Sugimoto, S., Yano, K., Hartzog, G. A., Winston, F., et al. (1998) · Genes & Development · doi:10.1101/gad.12.3.343 · Cited by 750
Inhibiting eukaryotic transcription: which compound to choose? How to evaluate its activity
Bensaude, O. (2011) · Transcription · doi:10.4161/trns.2.3.16172 · Cited by 574
Jordan, A., Defechereux, P., & Verdin, E. (2001) · The EMBO Journal · doi:10.1093/emboj/20.7.1726 · Cited by 467
Transcriptional and Posttranscriptional Regulation of HIV-1 Gene Expression
Karn, J., & Stoltzfus, C. M. (2012) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a006916 · Cited by 437
Multifaceted Activities of the HIV-1 Transactivator of Transcription, Tat
Jeang, K.-T., Xiao, H., & Rich, E. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.41.28837 · Cited by 414
Metadata
- Authors
- Mancebo, H. S. Y., Lee, G., Flygare, J., Tomassini, J., Luu, P., Zhu, Y., Peng, J., Blau, C., Hazuda, D., Price, D., & Flores, O.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1997-10-15
- Volume
- 11
- Issue
- 20
- Pages
- 2633-2644
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2108422586
- Cited by
- 544
- References
- 2
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro. https://app.cochat.ai/papers/10.1101/gad.11.20.2633
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- Mancebo, H. S. Y., Lee, G., Flygare, J., Tomassini, J., Luu, P., Zhu, Y., Peng, J., Blau, C., Hazuda, D., Price, D., & Flores, O. (1997). P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro. Genes & Development. https://doi.org/10.1101/gad.11.20.2633
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