The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment
Open accessAbstract
Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects of chromosome activity. Thr 3 of histone H3 is highly conserved, but the significance of its phosphorylation is unclear, and the identity of the corresponding kinase unknown. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr 3 in prophase and its dephosphorylation during anaphase. Furthermore we find that haspin, a member of a distinctive group of protein kinases present in diverse eukaryotes, phosphorylates H3 at Thr 3 in vitro. Importantly, depletion of haspin by RNA interference reveals that this kinase is required for H3 Thr 3 phosphorylation in mitotic cells. In addition to its chromosomal association, haspin is found at the centrosomes and spindle during mitosis. Haspin RNA interference causes misalignment of metaphase chromosomes, and overexpression delays progression through early mitosis. This work reveals a new kinase involved in composing the histone code and adds haspin to the select group of kinases that integrate regulation of chromosome and spindle function during mitosis and meiosis.
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Citations
Cites
Kinetics of Core Histones in Living Human Cells
Kimura, H., & Cook, P. R. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.7.1341 · Cited by 675
Cited by
Chromatin modifications and their function
Kouzarides, T. (2007) · Cell · doi:10.1016/j.cell.2007.02.005 · Cited by 10,883
“The study of such pathways is less detailed, but examples include H3K4me disrupting the binding of the NuRD complex and H3T3ph preventing the binding of the INHAT complex.”
Double chromodomains cooperate to recognize the methylated histone H3 tail
Flanagan, J. F., Mi, L.-Z., Chruszcz, M., Cymborowski, M., Clines, K. L., Kim, Y., Minor, W., Rastinejad, F., & Khorasanizadeh, S. (2005) · Nature · doi:10.1038/nature04290 · Cited by 527
Signals and Combinatorial Functions of Histone Modifications
Suganuma, T., & Workman, J. L. (2011) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-061809-175347 · Cited by 499
Carrasco, D. R., Tonon, G., Huang, Y., Zhang, Y., Sinha, R., Feng, B., Stewart, J. P., Zhan, F., Khatry, D., Protopopova, M., et al. (2006) · Cancer Cell · doi:10.1016/j.ccr.2006.03.019 · Cited by 425
Taverna, S. D., Ilin, S., Rogers, R. S., Tanny, J. C., Lavender, H., Li, H., Baker, L., Boyle, J., Blair, L. P., Chait, B. T., et al. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.10.026 · Cited by 332
Metadata
- Authors
- Dai, J., Sultan, S., Taylor, S. S., & Higgins, J. M. G.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2005-01-28
- Volume
- 19
- Issue
- 4
- Pages
- 472-488
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2124664751
- Cited by
- 372
- References
- 1
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Dai, J., Sultan, S., Taylor, S. S., & Higgins, J. M. G. (2005). The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Genes & Development. https://doi.org/10.1101/gad.1267105
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