The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Regulon and promoter analysis of the E. coli heat-shock factor, σ 32 , reveals a multifaceted cellular response to heat stress
Open accessAbstract
The heat-shock response (HSR), a universal cellular response to heat, is crucial for cellular adaptation. In Escherichia coli , the HSR is mediated by the alternative σ factor, σ 32 . To determine its role, we used genome-wide expression analysis and promoter validation to identify genes directly regulated by σ 32 and screened ORF overexpression libraries to identify σ 32 inducers. We triple the number of genes validated to be transcribed by σ 32 and provide new insights into the cellular role of this response. Our work indicates that the response is propagated as the regulon encodes numerous global transcriptional regulators, reveals that σ 70 holoenzyme initiates from 12% of σ 32 promoters, which has important implications for global transcriptional wiring, and identifies a new role for the response in protein homeostasis, that of protecting complex proteins. Finally, this study suggests that the response protects the cell membrane and responds to its status: Fully 25% of σ 32 regulon members reside in the membrane and alter its functionality; moreover, a disproportionate fraction of overexpressed proteins that induce the response are membrane localized. The intimate connection of the response to the membrane rationalizes why a major regulator of the response resides in that cellular compartment.
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1 retracted
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Cited by
EcoCyc: A comprehensive view of Escherichia coli biology
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“Examples include a collection of 54 s32 promoters experimentally identified by ChIP-chip and by gene expression assays (7); 45 s32 promoters identified by microarray analysis, transcription initiation mapping and computational analysis ( 8 ); and 45 Fur DNA binding sites identified by computational prediction and bind…”
BackgroundIdentification and characterization of E. coli CRISPR‐cas promoters and their silencing by H‐NS
Pul, Ü., Wurm, R., Arslan, Z., Geißen, R., Hofmann, N., & Wagner, R. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07073.x · Cited by 282
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Self-citation
Metadata
- Authors
- Nonaka, G., Blankschien, M., Herman, C., Gross, C. A., & Rhodius, V. A.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2006-07-01
- Volume
- 20
- Issue
- 13
- Pages
- 1776-1789
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2051048419
- Cited by
- 320
- References
- 74
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Nonaka, G., Blankschien, M., Herman, C., Gross, C. A., & Rhodius, V. A. (2006). Regulon and promoter analysis of the E. coli heat-shock factor, σ 32 , reveals a multifaceted cellular response to heat stress. Genes & Development. https://doi.org/10.1101/gad.1428206
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