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Cited by 100Genomics and Chromatin DynamicsChromatin Remodeling and CancerNuclear Structure and Function

Widespread changes in nucleosome accessibility without changes in nucleosome occupancy during a rapid transcriptional induction

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Mueller, Britta, Mieczkowski, Jakub, Kundu, Sharmistha, Wang, Peggy, Sadreyev, Ruslan, Tolstorukov, Michael Y., Kingston, Robert E.·2017·Genes & Development
DOI 10.1101/gad.293118.116PMC PMC5393060
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Abstract

Activation of transcription requires alteration of chromatin by complexes that increase the accessibility of nucleosomal DNA. Removing nucleosomes from regulatory sequences has been proposed to play a significant role in activation. We tested whether changes in nucleosome occupancy occurred on the set of genes that is activated by the unfolded protein response (UPR). We observed no decrease in occupancy on most promoters, gene bodies, and enhancers. Instead, there was an increase in the accessibility of nucleosomes, as measured by micrococcal nuclease (MNase) digestion and ATAC-seq (assay for transposase-accessible chromatin [ATAC] using sequencing), that did not result from removal of the nucleosome. Thus, changes in nucleosome accessibility predominate over changes in nucleosome occupancy during rapid transcriptional induction during the UPR.

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Cited by

Sorted by citations

Authors
Mueller, B., Mieczkowski, J., Kundu, S., Wang, P., Sadreyev, R., Tolstorukov, M. Y., & Kingston, R. E.
Affiliations
Harvard University (US)
ORCID
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
2017-03-01
Volume
31
Issue
5
Pages
451-462
ISSN
  • 0890-9369 (ISSN-L)
  • 1549-5477
DOI
10.1101/gad.293118.116
PMC
PMC5393060
OpenAlex
W2602182553
Cited by
100
References
2
Citations by year
2026: 4 · 2025: 3 · 2024: 3 · 2023: 9 · 2022: 6 · 2021: 16 · 2020: 16 · 2019: 21 · 2018: 17 · 2017: 4
Type
posted content
Language
en
Open access
Licence
cc-by
Locations
  • Genes & Development (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • National Institute of General Medical Sciences of the National Institutes of Health (R01GM043901, R37GM048405)
  • Leukemia and Lymphoma Society
  • National Institutes of Health (P30 DK040561)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
nucleosome accessibility, nucleosome occupancy, unfolded protein response, transcriptional induction, chromatin remodeling, transcriptional activation, enhancers, promoter, gene body, ATAC-seq
MeSH terms
Animals, Cell Line, Chromatin, Chromosome Mapping, Drosophila melanogaster, Enhancer Elements, Genetic, Gene Expression Regulation, Micrococcal Nuclease, Nucleosomes, Promoter Regions, Genetic, Protein Binding, Unfolded Protein Response

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